Human TFDP2 (DP2) knockout HEK-293T cell line
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TFDP2 KO cell line available to order. KO validated. Free of charge wild type control available. Knockout achieved by using CRISPR/Cas9, Homozygous: 1 bp insertion in exon 10. To order both knockout and wild-type control cells: select '2 x 1000000 Cells/vial'. To order only knockout cells: select '1000000 Cells/vial'.
View Alternative Names
E2F dimerization partner 2, TFDP2_HUMAN, Transcription factor Dp-2
- Sanger seq
Unknown
Sanger Sequencing - Human TFDP2 (DP2) knockout HEK-293T cell line (AB267291)
Homozygous : 1 bp insertion in exon10
Product details
We will provide viable cells that proliferate on revival.
This product is subject to limited use licenses from The Broad Institute, ERS Genomics Limited and Sigma-Aldrich Co. LLC, and is developed with patented technology. For full details of the licenses and patents please refer to our limited use license and patent pages.
What's included?
Properties and storage information
Gene name
Gene editing type
Gene editing method
Knockout validation
Zygosity
Shipped at conditions
Appropriate short-term storage conditions
Appropriate long-term storage conditions
Handling procedures
Initial handling guidelines
Upon arrival, the vial should be stored in liquid nitrogen vapor phase and not at -80°C. Storage at -80°C may result in loss of viability.
1. Thaw the vial in 37°C water bath for approximately 1-2 minutes.
2. Transfer the cell suspension (0.8 mL) to a 15 mL/50 mL conical sterile polypropylene centrifuge tube containing 8.4 mL pre-warmed culture medium, wash vial with an additional 0.8 mL culture medium (total volume 10 mL) to collect remaining cells, and centrifuge at 201 x g (rcf) for 5 minutes at room temperature. 10 mL represents minimum recommended dilution. 20 mL represents maximum recommended dilution.
3. Resuspend the cell pellet in 5 mL pre-warmed culture medium and count using a haemocytometer or alternative cell counting method seed all remaining cells into a T25.
4. Incubate the culture at 37°C incubator with 5% CO2. Check the culture one day after revival and continue to check until 80% confluent. Media change can be given if needed.
5. Once confluent passage into an appropriate flask at a density of 2x104 cells/cm2. Seeding density is given as a guide only and should be scaled to align with individual lab schedules. Cultures should be monitored daily.
Subculture guidelines
- All seeding densities should be based on cell counts gained by established methods.
- A guide seeding density of 2x104 cells/cm2 is recommended.
- Cells should be passaged when they have achieved 80-90% confluence.
Culture medium
DMEM (High Glucose) + 10% FBS
Cryopreservation medium
Cell Freezing Medium-DMSO Serum free media, contains 8.7% DMSO in MEM supplemented with methyl cellulose.
Supplementary information
This supplementary information is collated from multiple sources and compiled automatically.
Biological function summary
DP2 functions as a receptor for prostaglandin D2 (PGD2) an important lipid mediator in inflammation. DP2's activities are a part of PGD2-driven processes including the recruitment and activation of Th2 cells eosinophils and basophils which are integral to allergic responses. DP2 does not form part of a larger protein complex but operates in concert with prostanoids such as the D-type prostanoid (DP1) receptors highlighting its specific signaling pathway within the immune context. By mediating chemotaxis and activation of specific immune cells DP2 supports the perpetuation of Th2-type inflammation.
Pathways
DP2 plays a role in the prostaglandin signaling pathway and immune response network. It operates parallel to receptors like DP1 albeit delivering contrasting cellular outcomes. While DP1 typically signals via a cAMP-dependent pathway DP2 mediates its effects through calcium mobilization and beta-arrestin-dependent pathways. These pathways contribute to distinct yet overlapping roles in processes of allergy and inflammation illustrating how DP2 function is intricately connected with other signaling molecules including chemoattractant receptors involved in leukocyte trafficking.
Quality control
STR analysis
CSF1PO, D13S317, D7S820, D5S818, TH01, D16S539, TPOX
Cell culture
Biosafety level
EU: 2 US: 2
Adherent/suspension
Adherent
Gender
Female
Target data
Complementary Products
Product promise
Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.
For licensing inquiries, please contact partnerships@abcam.com