TFDP2 KO cell line available to order. Free of charge wild type control provided. Knockout achieved by using CRISPR/Cas9, Homozygous: 1 bp insertion in exon 10.
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Key facts
- Cell type
- HEK-293T
- Species or organism
- Human
- Tissue
- Kidney
- Form
- Liquid
- Knockout validation
- Sanger Sequencing
- Mutation description
- Knockout achieved by using CRISPR/Cas9, Homozygous: 1 bp insertion in exon 10
Alternative names
E2F dimerization partner 2, TFDP2_HUMAN, Transcription factor Dp-2
Recommended products
TFDP2 KO cell line available to order. Free of charge wild type control provided. Knockout achieved by using CRISPR/Cas9, Homozygous: 1 bp insertion in exon 10.
Key facts
- Cell type
- HEK-293T
- Species or organism
- Human
- Tissue
- Kidney
- Form
- Liquid
- Knockout validation
- Sanger Sequencing
- Mutation description
- Knockout achieved by using CRISPR/Cas9, Homozygous: 1 bp insertion in exon 10
- Concentration
Properties
- Gene name
- TFDP2
- Gene editing type
- Knockout
- Gene editing method
- CRISPR technology
- Knockout validation
- Sanger Sequencing
- Zygosity
- Homozygous
Quality control
- STR analysis
- CSF1PO, D13S317, D7S820, D5S818, TH01, D16S539, TPOX
Cell culture
- Biosafety level
- EU: 2 US: 2
- Adherent/suspension
- Adherent
- Gender
- Female
Handling procedures
- Initial handling guidelines
Upon arrival, the vial should be stored in liquid nitrogen vapor phase and not at -80°C. Storage at -80°C may result in loss of viability.
1. Thaw the vial in 37°C water bath for approximately 1-2 minutes.
2. Transfer the cell suspension (0.8 mL) to a 15 mL/50 mL conical sterile polypropylene centrifuge tube containing 8.4 mL pre-warmed culture medium, wash vial with an additional 0.8 mL culture medium (total volume 10 mL) to collect remaining cells, and centrifuge at 201 x g (rcf) for 5 minutes at room temperature. 10 mL represents minimum recommended dilution. 20 mL represents maximum recommended dilution.
3. Resuspend the cell pellet in 5 mL pre-warmed culture medium and count using a haemocytometer or alternative cell counting method seed all remaining cells into a T25.
4. Incubate the culture at 37°C incubator with 5% CO2. Check the culture one day after revival and continue to check until 80% confluent. Media change can be given if needed.
5. Once confluent passage into an appropriate flask at a density of 2x104 cells/cm2. Seeding density is given as a guide only and should be scaled to align with individual lab schedules. Cultures should be monitored daily.- Subculture guidelines
- All seeding densities should be based on cell counts gained by established methods.
- A guide seeding density of 2x104 cells/cm2 is recommended.
- Cells should be passaged when they have achieved 80-90% confluence.
- Culture medium
- DMEM (High Glucose) + 10% FBS
- Cryopreservation medium
- Cell Freezing Medium-DMSO Serum free media, contains 8.7% DMSO in MEM supplemented with methyl cellulose.
Storage
- Shipped at conditions
- Dry Ice
- Appropriate short-term storage conditions
- -196°C
- Appropriate long-term storage conditions
- -196°C
Notes
We will provide viable cells that proliferate on revival.
This product is subject to limited use licenses from The Broad Institute, ERS Genomics Limited and Sigma-Aldrich Co. LLC, and is developed with patented technology. For full details of the licenses and patents please refer to our limited use license and patent pages.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
DP2 also known as CRTH2 or GPR44 is a receptor from the class of G protein-coupled receptors (GPCRs). It is involved in the signaling pathways mediated by prostaglandins making it an essential mediator in inflammatory processes. The molecular mass of this receptor is approximately 44 kDa. DP2 is broadly expressed in various tissues including the lungs eosinophils Th2 cells and basophils highlighting its role in immune response and respiratory function. Its expression in numerous cell types underlines its versatile roles within the immune system.
- Biological function summary
DP2 functions as a receptor for prostaglandin D2 (PGD2) an important lipid mediator in inflammation. DP2's activities are a part of PGD2-driven processes including the recruitment and activation of Th2 cells eosinophils and basophils which are integral to allergic responses. DP2 does not form part of a larger protein complex but operates in concert with prostanoids such as the D-type prostanoid (DP1) receptors highlighting its specific signaling pathway within the immune context. By mediating chemotaxis and activation of specific immune cells DP2 supports the perpetuation of Th2-type inflammation.
- Pathways
DP2 plays a role in the prostaglandin signaling pathway and immune response network. It operates parallel to receptors like DP1 albeit delivering contrasting cellular outcomes. While DP1 typically signals via a cAMP-dependent pathway DP2 mediates its effects through calcium mobilization and beta-arrestin-dependent pathways. These pathways contribute to distinct yet overlapping roles in processes of allergy and inflammation illustrating how DP2 function is intricately connected with other signaling molecules including chemoattractant receptors involved in leukocyte trafficking.
- Associated diseases and disorders
The dysregulation of DP2 is associated with asthma and allergic rhinitis. Within the context of asthma DP2 expression correlates with severity and exacerbation of the disease. The receptor's role in mediating eosinophil and Th2 cell attraction makes it a target of interest for therapeutic interventions. In allergic rhinitis similar mechanisms involving DP2-mediated immune cell recruitment exacerbate mucosal inflammation during allergen exposure. DP2's interaction with other inflammatory mediators such as the cysteinyl leukotriene receptor family solidifies its position in the cascade of allergic and respiratory disorders.
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1 product image
Sanger Sequencing - Human TFDP2 (DP2) knockout HEK-293T cell line (ab267291)
Homozygous: 1 bp insertion in exon10
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