Human TGFBR1 knockout A549 cell line
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TGFBR1 KO cell line available to order. KO validated by. Free of charge wild type control provided.
View Alternative Names
AAT 5, ACVRLK 4, ALK-5, Activin A receptor type II like kinase, Activin A receptor type II like kinase 53kDa, Activin A receptor type II like kinase, 53kD, Activin receptor-like kinase 5, LDS1A, LDS2A, MSSE, SKR 4, Serine/threonine-protein kinase receptor R4, TGF-beta receptor type I, TGF-beta receptor type-1, TGF-beta type I receptor, TGFBR 1, TGFBR1 protein, TGFR1_HUMAN, TbetaR-I, Transforming growth factor beta receptor 1, Transforming growth factor beta receptor I, Transforming growth factor beta receptor I (activin A receptor type II like kinase, 53kD), Transforming growth factor-beta receptor type I, activin A receptor type II-like protein kinase of 53kD
- WB
Lab
Western blot - Human TGFBR1 knockout A549 cell line (AB277894)
False colour image of Western blot : Anti-TGF beta Receptor I antibody [EPR20923-13] staining at 1/1000 dilution shown in green; Mouse anti-GAPDH antibody [6C5] (ab8245) loading control staining at 1/20000 dilution shown in red. In Western blot ab235578 was shown to bind specifically to TGF beta Receptor I. A band was observed at 40/55 kDa in wild-type A549 cell lysates with no signal observed at this size in TGFBR1 knockout cell line ab277894 (knockout cell lysate ab283082). To generate this image wild-type and TGFBR1 knockout A549 cell lysates were analysed. First samples were run on an SDS-PAGE gel then transferred onto a nitrocellulose membrane. Membranes were blocked in 3 % milk in TBS-0.1 % Tween® 20 (TBS-T) before incubation with primary antibodies overnight at 4 °. Blots were washed four times in TBS-T incubated with secondary antibodies for 1 h at room temperature washed again four times then imaged. Secondary antibodies used were Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed (ab216776) at 1/20000 dilution.
All lanes:
Western blot - Anti-TGF beta Receptor I antibody [EPR20923-13] (<a href='/en-us/products/primary-antibodies/tgf-beta-receptor-i-antibody-epr20923-13-ab235578'>ab235578</a>) at 1/1000 dilution
Lane 1:
Wild-type A549 cell lysate at 20 µg
Lane 2:
Western blot - Human TGFBR1 knockout A549 cell lysate (ab283082) at 20 µg
Lane 3:
A431 cell lysate at 20 µg
Lane 4:
MOLT-4 cell lysate at 20 µg
Predicted band size: 55 kDa
Observed band size: 40 kDa,55 kDa
false
Product details
Recommended control: Human wild-type A549 cell line (ab255450). Please note a wild-type cell line is not automatically included with a knockout cell line order, if required please add recommended wild-type cell line at no additional cost using the code WILDTYPE-TMTK1.
We will provide viable cells that proliferate on revival.
This product is subject to limited use licenses from The Broad Institute and ERS Genomics Limited, and is developed with patented technology. For full details of the limited use licenses and relevant patents please refer to our limited use license and patent pages.
What's included?
Properties and storage information
Gene name
Gene editing type
Gene editing method
Shipped at conditions
Appropriate short-term storage conditions
Appropriate long-term storage conditions
Handling procedures
Initial handling guidelines
Upon arrival, the vial should be stored in liquid nitrogen vapor phase and not at -80°C. Storage at -80°C may result in loss of viability.
1. Thaw the vial in 37°C water bath for approximately 1-2 minutes.
2. Transfer the cell suspension (0.8 mL) to a 15 mL/50 mL conical sterile polypropylene centrifuge tube containing 8.4 mL pre-warmed culture medium, wash vial with an additional 0.8 mL culture medium (total volume 10 mL) to collect remaining cells, and centrifuge at 201 x g (rcf) for 5 minutes at room temperature. 10 mL represents minimum recommended dilution. 20 mL represents maximum recommended dilution.
3. Resuspend the cell pellet in 5 mL pre-warmed culture medium and count using a haemocytometer or alternative cell counting method seed all remaining cells into a T25.
4. Incubate the culture at 37°C incubator with 5% CO2. Check the culture one day after revival and continue to check until 80% confluent. Media change can be given if needed.
5. Once confluent passage into an appropriate flask at a density of 2x104 cells/cm2. Seeding density is given as a guide only and should be scaled to align with individual lab schedules. Cultures should be monitored daily.
Subculture guidelines
- All seeding densities should be based on cell counts gained by established methods.
- A guide seeding density of 2x104 cells/cm2 is recommended.
- Cells should be passaged when they have achieved 80-90% confluence.
- Do not allow the cell density to exceed 7x104 cells/cm2.
Culture medium
F-12K + 10% FBS
Cryopreservation medium
Cell Freezing Medium-DMSO Serum free media, contains 8.7% DMSO in MEM supplemented with methyl cellulose.
Quality control
STR analysis
CSF1PO, D13S317, D7S820, D5S818, TH01, D16S539, TPOX
Cell culture
Biosafety level
EU: 1 US: 1
Adherent/suspension
Adherent
Target data
Complementary Products
Primary Antibodies
AB235578
Anti-TGF beta Receptor I antibody [EPR20923-13]
20ul selling size|RabMAb|Recombinant|KO Validated
![Western blot - Anti-TGF beta Receptor I antibody [EPR20923-13] (AB235578)](https://content.abcam.com/products/images/tgf-beta-receptor-i-antibody-epr20923-13-ab235578--western-blot-img171134.jpg)
- 0
- 0
Primary Antibodies
AB40854
20ul selling size|Recombinant|KO Validated|Advanced Validation|RabMAb
![Western blot - Anti-Smad3 antibody [EP568Y] (AB40854)](https://content.abcam.com/products/images/smad3-antibody-ep568y-ab40854--western-blot-img140787.jpg)
- 5
- 10
Product promise
Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.
For licensing inquiries, please contact partnerships@abcam.com