TIMM17B KO cell line available to order. Free of charge wild type control provided. Knockout achieved by using CRISPR/Cas9, 13 bp deletion in exon 3 and Insertion of the selection cassette in exon 3.
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Key facts
- Cell type
- HEK-293T
- Species or organism
- Human
- Tissue
- Kidney
- Form
- Liquid
- Knockout validation
- Sanger Sequencing
- Mutation description
- Knockout achieved by using CRISPR/Cas9, 13 bp deletion in exon 3 and Insertion of the selection cassette in exon 3
Alternative names
DXS9822, Inner mitochondrial membrane preprotein translocase, JM3, Mitochondrial import inner membrane translocase subunit Tim17-B, TI17B_HUMAN, Tim17b, Translocase of inner mitochondrial membrane 17 homolog B (yeast)
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TIMM17B KO cell line available to order. Free of charge wild type control provided. Knockout achieved by using CRISPR/Cas9, 13 bp deletion in exon 3 and Insertion of the selection cassette in exon 3.
Key facts
- Cell type
- HEK-293T
- Species or organism
- Human
- Tissue
- Kidney
- Form
- Liquid
- Knockout validation
- Sanger Sequencing
- Mutation description
- Knockout achieved by using CRISPR/Cas9, 13 bp deletion in exon 3 and Insertion of the selection cassette in exon 3
- Concentration
Properties
- Gene name
- TIMM17B
- Gene editing type
- Knockout
- Gene editing method
- CRISPR technology
- Knockout validation
- Sanger Sequencing
Quality control
- STR analysis
- CSF1PO, D13S317, D7S820, D5S818, TH01, D16S539, TPOX
Cell culture
- Biosafety level
- EU: 2 US: 2
- Adherent/suspension
- Adherent
- Gender
- Female
Handling procedures
- Initial handling guidelines
Upon arrival, the vial should be stored in liquid nitrogen vapor phase and not at -80°C. Storage at -80°C may result in loss of viability.
1. Thaw the vial in 37°C water bath for approximately 1-2 minutes.
2. Transfer the cell suspension (0.8 mL) to a 15 mL/50 mL conical sterile polypropylene centrifuge tube containing 8.4 mL pre-warmed culture medium, wash vial with an additional 0.8 mL culture medium (total volume 10 mL) to collect remaining cells, and centrifuge at 201 x g (rcf) for 5 minutes at room temperature. 10 mL represents minimum recommended dilution. 20 mL represents maximum recommended dilution.
3. Resuspend the cell pellet in 5 mL pre-warmed culture medium and count using a haemocytometer or alternative cell counting method seed all remaining cells into a T25.
4. Incubate the culture at 37°C incubator with 5% CO2. Check the culture one day after revival and continue to check until 80% confluent. Media change can be given if needed.
5. Once confluent passage into an appropriate flask at a density of 2x104 cells/cm2. Seeding density is given as a guide only and should be scaled to align with individual lab schedules. Cultures should be monitored daily.- Subculture guidelines
- All seeding densities should be based on cell counts gained by established methods.
- A guide seeding density of 2x104 cells/cm2 is recommended.
- Cells should be passaged when they have achieved 80-90% confluence.
- Culture medium
- DMEM (High Glucose) + 10% FBS
- Cryopreservation medium
- Cell Freezing Medium-DMSO Serum free media, contains 8.7% DMSO in MEM supplemented with methyl cellulose.
Storage
- Shipped at conditions
- Dry Ice
- Appropriate short-term storage conditions
- -196°C
- Appropriate long-term storage conditions
- -196°C
Notes
We will provide viable cells that proliferate on revival.
This product is subject to limited use licenses from The Broad Institute, ERS Genomics Limited and Sigma-Aldrich Co. LLC, and is developed with patented technology. For full details of the licenses and patents please refer to our limited use license and patent pages.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
TIMM17B also known as Translocase of Inner Mitochondrial Membrane 17 homolog B is a protein component with a molecular mass of approximately 21 kDa. TIMM17B is importantly involved in the translocation of proteins across the mitochondrial inner membrane. This protein is expressed in various tissues but shows higher expression in testis and heart. TIMM17B plays a critical role in mitochondrial function by acting as a channel-forming protein within the TIMM complex.
- Biological function summary
TIMM17B integrates its function into the import machinery for mitochondrial proteins. It is part of the TIM22 complex an important multi-protein system that mediates the insertion and assembly of certain hydrophobic proteins into the mitochondrial inner membrane. TIMM17B works in coordination with other TIMM proteins such as TIM22 to facilitate the transfer and integration of mitochondrial carrier proteins. This integration supports the effective maintenance of mitochondrial functions which are essential for cellular energy metabolism.
- Pathways
TIMM17B plays a significant role in the mitochondrial protein import pathway. This process involves the transport of proteins synthesized in the cytosol into the mitochondria which is essential for mitochondrial biogenesis and function. TIMM17B is also linked with the oxidative phosphorylation pathway through its involvement in maintaining mitochondrial membrane potential. Proteins like TIMM23 and TIMM50 are closely related to TIMM17B within these pathways providing important support for its function in importing proteins necessary for energy production.
- Associated diseases and disorders
TIMM17B has been connected to mitochondrial disorders and neurodegenerative diseases. Disturbances in its function or expression can affect mitochondrial integrity contributing to conditions such as Leigh syndrome. Moreover TIMM17B shows associations with Alzheimer's disease where defects in mitochondrial dynamics and energy metabolism play a role. These connections also involve the protein PINK1 which is associated with autosomal recessive early-onset Parkinson's disease highlighting the importance of mitochondrial quality control in neurodegenerative disorders.
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2 product images
Sanger Sequencing - Human TIMM17B knockout HEK-293T cell line (ab266566)
Allele-1: Insertion of the selection cassette in exon3
Sanger Sequencing - Human TIMM17B knockout HEK-293T cell line (ab266566)
Allele-2: 13 bp deletion in exon 3.
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