TM4SF1 KO cell line available to order. Free of charge wild type control provided. Knockout achieved by using CRISPR/Cas9, 0 bp insertion in exon 1 and 1 bp insertion in exon 1.
Images
Key facts
- Cell type
HeLa
- Species or organism
Human
- Tissue
Cervix
- Form
Liquid
- Knockout validation
Sanger Sequencing
- Mutation description
Knockout achieved by using CRISPR/Cas9, 0 bp insertion in exon 1 and 1 bp insertion in exon 1
Alternative names
M3S1, Membrane component chromosome 3 surface marker 1, T4S1_HUMAN, TAAL6, Tm4sf1, Transmembrane 4 L6 family member 1, Tumor-associated antigen L6
Recommended products
TM4SF1 KO cell line available to order. Free of charge wild type control provided. Knockout achieved by using CRISPR/Cas9, 0 bp insertion in exon 1 and 1 bp insertion in exon 1.
Key facts
- Cell type
HeLa
- Species or organism
Human
- Tissue
Cervix
- Form
Liquid
- Knockout validation
Sanger Sequencing
- Mutation description
Knockout achieved by using CRISPR/Cas9, 0 bp insertion in exon 1 and 1 bp insertion in exon 1
- Disease
Adenocarcinoma
- Concentration
Properties
- Gene name
TM4SF1
- Gene editing type
Knockout
- Gene editing method
CRISPR technology
- Knockout validation
Sanger Sequencing
Quality control
- STR analysis
CSF1PO, D13S317, D7S820, D5S818, TH01, D16S539, TPOX
Cell culture
- Biosafety level
EU: 2 US: 2
- Adherent/suspension
Adherent
- Gender
Female
Handling procedures
- Initial handling guidelines
Upon arrival, the vial should be stored in liquid nitrogen vapor phase and not at -80°C. Storage at -80°C may result in loss of viability.
1. Thaw the vial in 37°C water bath for approximately 1-2 minutes.
2. Transfer the cell suspension (0.8 mL) to a 15 mL/50 mL conical sterile polypropylene centrifuge tube containing 8.4 mL pre-warmed culture medium, wash vial with an additional 0.8 mL culture medium (total volume 10 mL) to collect remaining cells, and centrifuge at 201 x g (rcf) for 5 minutes at room temperature. 10 mL represents minimum recommended dilution. 20 mL represents maximum recommended dilution.
3. Resuspend the cell pellet in 5 mL pre-warmed culture medium and count using a haemocytometer or alternative cell counting method seed all remaining cells into a T25.
4. Incubate the culture at 37°C incubator with 5% CO2. Check the culture one day after revival and continue to check until 80% confluent. Media change can be given if needed.
5. Once confluent passage into an appropriate flask at a density of 2x104 cells/cm2. Seeding density is given as a guide only and should be scaled to align with individual lab schedules. Cultures should be monitored daily.- Subculture guidelines
- All seeding densities should be based on cell counts gained by established methods.
- A guide seeding density of 2x104 cells/cm2 is recommended.
- Cells should be passaged when they have achieved 80-90% confluence.
- Culture medium
DMEM (High Glucose) + 10% FBS
- Cryopreservation medium
Cell Freezing Medium-DMSO Serum free media, contains 8.7% DMSO in MEM supplemented with methyl cellulose.
Storage
- Shipped at conditions
Dry Ice
- Appropriate short-term storage conditions
-196°C
- Appropriate long-term storage conditions
-196°C
Notes
Recommended control: Human wild-type HeLa cell line (Human wild-type HeLa cell line ab255928). Please note a wild-type cell line is not automatically included with a knockout cell line order, if required please add recommended wild-type cell line at no additional cost using the code WILDTYPE-TMTK1.
We will provide viable cells that proliferate on revival.
This product is subject to limited use licenses from The Broad Institute, ERS Genomics Limited and Sigma-Aldrich Co. LLC, and is developed with patented technology. For full details of the licenses and patents please refer to our limited use license and patent pages.
Supplementary info
This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
Transmembrane 4 L6 family member 1 also called TM4SF1 is a protein with an approximate mass of 23 kDa. It is a member of the tetraspanin superfamily and consists of four transmembrane domains. TM4SF1 is mostly expressed in epithelial and endothelial cells with higher levels seen in many cancerous tissues. This protein integrates into cell membranes where it plays a role in cellular communication by interacting with other membrane proteins.
- Biological function summary
TM4SF1 is involved in regulating diverse cellular processes such as proliferation migration and angiogenesis. It is not part of any larger complex but works through its interactions with integrins growth factor receptors and other tetraspanins. The presence of TM4SF1 impacts the organization of lipid rafts facilitating cell signaling pathways essential for cell growth and survival.
- Pathways
TM4SF1 functions in both the PI3K/AKT and MAPK signaling cascades. These pathways are essential for controlling cell proliferation and survival. TM4SF1 influences the activity of proteins such as AKT and ERK which are important in mediating responses to growth factor signals and cellular stress. Its interactions with integrins also modulate signaling and structural dynamics of the cellular environment.
- Associated diseases and disorders
TM4SF1 is linked to several cancers including breast and lung cancer. Its elevated expression levels contribute to tumor growth and metastasis through enhancement of cell migration and angiogenesis. TM4SF1 is also associated with abnormal endothelial cell function influencing angiogenic diseases through its connections with proteins like VEGFR2 and integrin αVβ3. Understanding TM4SF1's role in these conditions can assist in developing therapeutic strategies.
Product promise
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In the unlikely event of one of our products not working as expected, you are covered by our product promise.
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4 product images
Sanger Sequencing - Human TM4SF1 (Transmembrane 4 L6 family member 1) knockout HeLa cell line (ab265743)
Allele-2: 1 bp insertion in exon 1.
Sanger Sequencing - Human TM4SF1 (Transmembrane 4 L6 family member 1) knockout HeLa cell line (ab265743)
Allele-1: 0 bp insertion in exon 1.
Sanger Sequencing - Human TM4SF1 (Transmembrane 4 L6 family member 1) knockout HeLa cell line (ab265743)
Sanger Sequencing - Human TM4SF1 (Transmembrane 4 L6 family member 1) knockout HeLa cell line (ab265743)
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