TM9SF4 KO cell line available to order. Free of charge wild type control provided. Knockout achieved by using CRISPR/Cas9, Homozygous: 1 bp insertion in exon 2.
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Key facts
- Cell type
- HeLa
- Species or organism
- Human
- Tissue
- Cervix
- Form
- Liquid
- Knockout validation
- Sanger Sequencing
- Mutation description
- Knockout achieved by using CRISPR/Cas9, Homozygous: 1 bp insertion in exon 2
Alternative names
KIAA0255, TM9S4_HUMAN, Transmembrane 9 superfamily member 4, Transmembrane 9 superfamily protein member 4
Recommended products
TM9SF4 KO cell line available to order. Free of charge wild type control provided. Knockout achieved by using CRISPR/Cas9, Homozygous: 1 bp insertion in exon 2.
Key facts
- Cell type
- HeLa
- Species or organism
- Human
- Tissue
- Cervix
- Form
- Liquid
- Knockout validation
- Sanger Sequencing
- Mutation description
- Knockout achieved by using CRISPR/Cas9, Homozygous: 1 bp insertion in exon 2
- Disease
- Adenocarcinoma
- Concentration
Properties
- Gene name
- TM9SF4
- Gene editing type
- Knockout
- Gene editing method
- CRISPR technology
- Knockout validation
- Sanger Sequencing
- Zygosity
- Homozygous
Quality control
- STR analysis
- CSF1PO, D13S317, D7S820, D5S818, TH01, D16S539, TPOX
Cell culture
- Biosafety level
- EU: 2 US: 2
- Adherent/suspension
- Adherent
- Gender
- Female
Handling procedures
- Initial handling guidelines
Upon arrival, the vial should be stored in liquid nitrogen vapor phase and not at -80°C. Storage at -80°C may result in loss of viability.
1. Thaw the vial in 37°C water bath for approximately 1-2 minutes.
2. Transfer the cell suspension (0.8 mL) to a 15 mL/50 mL conical sterile polypropylene centrifuge tube containing 8.4 mL pre-warmed culture medium, wash vial with an additional 0.8 mL culture medium (total volume 10 mL) to collect remaining cells, and centrifuge at 201 x g (rcf) for 5 minutes at room temperature. 10 mL represents minimum recommended dilution. 20 mL represents maximum recommended dilution.
3. Resuspend the cell pellet in 5 mL pre-warmed culture medium and count using a haemocytometer or alternative cell counting method seed all remaining cells into a T25.
4. Incubate the culture at 37°C incubator with 5% CO2. Check the culture one day after revival and continue to check until 80% confluent. Media change can be given if needed.
5. Once confluent passage into an appropriate flask at a density of 2x104 cells/cm2. Seeding density is given as a guide only and should be scaled to align with individual lab schedules. Cultures should be monitored daily.- Subculture guidelines
- All seeding densities should be based on cell counts gained by established methods.
- A guide seeding density of 2x104 cells/cm2 is recommended.
- Cells should be passaged when they have achieved 80-90% confluence.
- Culture medium
- DMEM (High Glucose) + 10% FBS
- Cryopreservation medium
- Cell Freezing Medium-DMSO Serum free media, contains 8.7% DMSO in MEM supplemented with methyl cellulose.
Storage
- Shipped at conditions
- Dry Ice
- Appropriate short-term storage conditions
- -196°C
- Appropriate long-term storage conditions
- -196°C
Notes
We will provide viable cells that proliferate on revival.
This product is subject to limited use licenses from The Broad Institute, ERS Genomics Limited and Sigma-Aldrich Co. LLC, and is developed with patented technology. For full details of the licenses and patents please refer to our limited use license and patent pages.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
TM9SF4 also known as Transmembrane 9 Superfamily Member 4 is a multi-pass membrane protein with an approximate molecular mass of 70 kDa. It is localized in the Golgi apparatus and endosomes showing a notable expression in immune-related cells such as macrophages and dendritic cells. TM9SF4 is involved in the transport and sorting of proteins within cellular compartments playing an important role in maintaining intracellular pH homeostasis by modulating ion exchange across membranes.
- Biological function summary
As part of a larger protein family TM9SF4 is involved in the regulation of cellular processes like endocytosis and exocytosis. It functions as a regulator of phagocytosis a process critical for immune response and cell clearance. Through its action in cellular compartments it contributes to antigen presentation thereby influencing cellular immune responses. Its involvement in these pathways highlights its significance in both normal cellular functions and immune system regulation.
- Pathways
TM9SF4 influences processes associated with the endocytic pathway and vesicular trafficking. It interacts within cellular networks involving SNARE proteins responsible for vesicle fusion highlighting its role in transport pathways. TM9SF4 also associates with V-ATPase complexes affecting acidic environments necessary for hydrolytic enzymes in endosomes. Through these connections TM9SF4 helps sustain cellular dynamics and integrity.
- Associated diseases and disorders
TM9SF4 links to immune-related conditions particularly chronic inflammatory diseases. It affects macrophage function and inflammation making it relevant in disorders such as rheumatoid arthritis. Additionally TM9SF4 alterations associate with tumor microenvironments influencing cancer progression. Through its modulation of phagocytic functions abnormalities in TM9SF4 activity can alter interactions with proteins like TNF-alpha which are important in inflammation and cancer-related pathways.
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1 product image
Sanger Sequencing - Human TM9SF4 knockout HeLa cell line (ab265870)
Homozygous: 1 bp insertion in exon 2.
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