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TMEM140 KO cell line available to order. Free of charge wild type control provided. Knockout achieved by using CRISPR/Cas9, 1 bp deletion in exon 2 and 4 bp deletion in exon 2 and 8 bp deletion in exon 2.

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Images

Sanger Sequencing - Human TMEM140 knockout A549 cell line (AB266943), expandable thumbnail
  • Sanger Sequencing - Human TMEM140 knockout A549 cell line (AB266943), expandable thumbnail
  • Sanger Sequencing - Human TMEM140 knockout A549 cell line (AB266943), expandable thumbnail

Key facts

Cell type

A549

Species or organism

Human

Tissue

Lung

Form

Liquid

Knockout validation

Sanger Sequencing

Mutation description

Knockout achieved by using CRISPR/Cas9, 1 bp deletion in exon 2 and 4 bp deletion in exon 2 and 8 bp deletion in exon 2

Alternative names

Recommended products

TMEM140 KO cell line available to order. Free of charge wild type control provided. Knockout achieved by using CRISPR/Cas9, 1 bp deletion in exon 2 and 4 bp deletion in exon 2 and 8 bp deletion in exon 2.

Key facts

Cell type

A549

Form

Liquid

Mutation description

Knockout achieved by using CRISPR/Cas9, 1 bp deletion in exon 2 and 4 bp deletion in exon 2 and 8 bp deletion in exon 2

Disease

Carcinoma

Concentration
Loading...

Properties

Gene name

TMEM140

Gene editing type

Knockout

Gene editing method

CRISPR technology

Knockout validation

Sanger Sequencing

Quality control

STR analysis

CSF1PO, D13S317, D7S820, D5S818, TH01, D16S539, TPOX

Cell culture

Biosafety level

EU: 1 US: 1

Adherent/suspension

Adherent

Gender

Male

Handling procedures

Initial handling guidelines

Upon arrival, the vial should be stored in liquid nitrogen vapor phase and not at -80°C. Storage at -80°C may result in loss of viability.

1. Thaw the vial in 37°C water bath for approximately 1-2 minutes.
2. Transfer the cell suspension (0.8 mL) to a 15 mL/50 mL conical sterile polypropylene centrifuge tube containing 8.4 mL pre-warmed culture medium, wash vial with an additional 0.8 mL culture medium (total volume 10 mL) to collect remaining cells, and centrifuge at 201 x g (rcf) for 5 minutes at room temperature. 10 mL represents minimum recommended dilution. 20 mL represents maximum recommended dilution.
3. Resuspend the cell pellet in 5 mL pre-warmed culture medium and count using a haemocytometer or alternative cell counting method seed all remaining cells into a T25.
4. Incubate the culture at 37°C incubator with 5% CO2. Check the culture one day after revival and continue to check until 80% confluent. Media change can be given if needed.
5. Once confluent passage into an appropriate flask at a density of 2x104 cells/cm2. Seeding density is given as a guide only and should be scaled to align with individual lab schedules. Cultures should be monitored daily.

Subculture guidelines

  • All seeding densities should be based on cell counts gained by established methods.

  • A guide seeding density of 2x104 cells/cm2 is recommended.

  • Cells should be passaged when they have achieved 80-90% confluence.

  • Do not allow the cell density to exceed 7x104 cells/cm2.

Culture medium

F-12K + 10% FBS

Cryopreservation medium

Cell Freezing Medium-DMSO Serum free media, contains 8.7% DMSO in MEM supplemented with methyl cellulose.

Storage

Shipped at conditions

Dry Ice

Appropriate short-term storage conditions

-196°C

Appropriate long-term storage conditions

-196°C

Notes

Recommended control: Human wild-type A549 cell line (Human wild-type A549 cell line ab255450). Please note a wild-type cell line is not automatically included with a knockout cell line order, if required please add recommended wild-type cell line at no additional cost using the code WILDTYPE-TMTK1.

We will provide viable cells that proliferate on revival.

This product is subject to limited use licenses from The Broad Institute and ERS Genomics Limited, and is developed with patented technology. For full details of the limited use licenses and relevant patents please refer to our limited use license and patent pages.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.

Activity summary

TMEM140 also known as Transmembrane Protein 140 is a membrane protein with an approximate mass of 31 kDa. This protein spans the cell membrane and currently researchers identify its presence in various tissues including the brain and testis. TMEM140 involves in cellular signal transduction due to its integral membrane position and though the precise mechanical action remains under exploration experts suspect it plays a part in intracellular communication.

Biological function summary

TMEM140 impacts cell processes involving cellular differentiation and development. The protein does not traditionally belong to any larger multiprotein complex but preliminary evidence suggests it may interact with specific signaling molecules. Its expression and function imply potential roles in neurogenesis and gametogenesis contributing to the broader understanding of development.

Pathways

TMEM140 connects with pathways involving neural development and reproductive processes. It appears to interact with proteins related to these pathways which include other transmembrane proteins integral to cellular signaling. Experts study its involvement within MAPK signaling cascades a pathway important for transmitting extracellular signals to the cell nucleus affecting processes like growth and differentiation.

Associated diseases and disorders

Alterations in TMEM140 function link to neurodegenerative disorders like Alzheimer's disease and certain fertility issues. The protein interacts with proteins such as tau in neuronal contexts which relates to neurodegeneration. In reproductive health aberrant TMEM140 expression might affect proteins in the spermatogenesis pathway potentially leading to infertility.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

3 product images

  • Sanger Sequencing - Human TMEM140 knockout A549 cell line (ab266943), expandable thumbnail

    Sanger Sequencing - Human TMEM140 knockout A549 cell line (ab266943)

    Allele-1: 8 bp deletion in exon2

  • Sanger Sequencing - Human TMEM140 knockout A549 cell line (ab266943), expandable thumbnail

    Sanger Sequencing - Human TMEM140 knockout A549 cell line (ab266943)

    Allele-2: 4 bp deletion in exon 2.

  • Sanger Sequencing - Human TMEM140 knockout A549 cell line (ab266943), expandable thumbnail

    Sanger Sequencing - Human TMEM140 knockout A549 cell line (ab266943)

    Allele-3: 1 bp deletion in exon 2.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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