TMEM208 KO cell line available to order. Free of charge wild type control provided. Knockout achieved by using CRISPR/Cas9, 1 bp insertion in exon 1 and 41 bp insertion in exon 1 and 7 bp deletion in exon 1.
Images
Key facts
- Cell type
- HEK-293T
- Species or organism
- Human
- Tissue
- Kidney
- Form
- Liquid
- Knockout validation
- Sanger Sequencing
- Mutation description
- Knockout achieved by using CRISPR/Cas9, 1 bp insertion in exon 1 and 41 bp insertion in exon 1 and 7 bp deletion in exon 1
Alternative names
HSPC171, TM208_HUMAN, Transmembrane protein 208
Recommended products
TMEM208 KO cell line available to order. Free of charge wild type control provided. Knockout achieved by using CRISPR/Cas9, 1 bp insertion in exon 1 and 41 bp insertion in exon 1 and 7 bp deletion in exon 1.
Key facts
- Cell type
- HEK-293T
- Species or organism
- Human
- Tissue
- Kidney
- Form
- Liquid
- Knockout validation
- Sanger Sequencing
- Mutation description
- Knockout achieved by using CRISPR/Cas9, 1 bp insertion in exon 1 and 41 bp insertion in exon 1 and 7 bp deletion in exon 1
- Concentration
Properties
- Gene name
- TMEM208
- Gene editing type
- Knockout
- Gene editing method
- CRISPR technology
- Knockout validation
- Sanger Sequencing
Quality control
- STR analysis
- CSF1PO, D13S317, D7S820, D5S818, TH01, D16S539, TPOX
Cell culture
- Biosafety level
- EU: 2 US: 2
- Adherent/suspension
- Adherent
- Gender
- Female
Handling procedures
- Initial handling guidelines
Upon arrival, the vial should be stored in liquid nitrogen vapor phase and not at -80°C. Storage at -80°C may result in loss of viability.
1. Thaw the vial in 37°C water bath for approximately 1-2 minutes.
2. Transfer the cell suspension (0.8 mL) to a 15 mL/50 mL conical sterile polypropylene centrifuge tube containing 8.4 mL pre-warmed culture medium, wash vial with an additional 0.8 mL culture medium (total volume 10 mL) to collect remaining cells, and centrifuge at 201 x g (rcf) for 5 minutes at room temperature. 10 mL represents minimum recommended dilution. 20 mL represents maximum recommended dilution.
3. Resuspend the cell pellet in 5 mL pre-warmed culture medium and count using a haemocytometer or alternative cell counting method seed all remaining cells into a T25.
4. Incubate the culture at 37°C incubator with 5% CO2. Check the culture one day after revival and continue to check until 80% confluent. Media change can be given if needed.
5. Once confluent passage into an appropriate flask at a density of 2x104 cells/cm2. Seeding density is given as a guide only and should be scaled to align with individual lab schedules. Cultures should be monitored daily.- Subculture guidelines
- All seeding densities should be based on cell counts gained by established methods.
- A guide seeding density of 2x104 cells/cm2 is recommended.
- Cells should be passaged when they have achieved 80-90% confluence.
- Culture medium
- DMEM (High Glucose) + 10% FBS
- Cryopreservation medium
- Cell Freezing Medium-DMSO Serum free media, contains 8.7% DMSO in MEM supplemented with methyl cellulose.
Storage
- Shipped at conditions
- Dry Ice
- Appropriate short-term storage conditions
- -196°C
- Appropriate long-term storage conditions
- -196°C
Notes
We will provide viable cells that proliferate on revival.
This product is subject to limited use licenses from The Broad Institute, ERS Genomics Limited and Sigma-Aldrich Co. LLC, and is developed with patented technology. For full details of the licenses and patents please refer to our limited use license and patent pages.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
TMEM208 also known as transmembrane protein 208 plays a role in cellular processes involving the endoplasmic reticulum and lysosomes. With an estimated molecular weight of around 24 kDa scientist can find TMEM208 predominantly expressed in human tissues particularly in the heart and skeletal muscle. As a multi-pass membrane protein TMEM208 establishes the cell organelles' communication ensuring intracellular stability and proper functioning of cellular processes.
- Biological function summary
The protein serves an essential function in the formation and maintenance of autophagic vesicles. It operates as a component of the endoplasmic reticulum-Golgi intermediate compartment (ERGIC) participating in cargo sorting and transport. TMEM208's role in vesicle nucleation is important for autophagy a process where cells remove unnecessary or dysfunctional components maintaining cellular homeostasis. This action indirectly supports cellular adaptation to stress.
- Pathways
TMEM208 integrates into intracellular trafficking and autophagy pathways working alongside proteins such as LC3 and ATG-related proteins. Through its involvement in vesicular transport TMEM208 collaborates in the lysosomal degradation pathway a process important for recycling cellular materials. TMEM208's interaction with LC3 connects it to the macroautophagy pathway allowing the cell to respond effectively to nutrient deprivation or other cellular stressors.
- Associated diseases and disorders
TMEM208 associates with pathologies linked to improper autophagic processes and cellular stress responses including neurodegenerative diseases like Alzheimer's and possibly myopathies. Researchers note its association with proteins such as p62 which accumulates when autophagy is disrupted indicating TMEM208's potential role in disease progression. Understanding TMEM208's function might lead to identifying therapeutic targets for these conditions.
Product promise
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3 product images
Sanger Sequencing - Human TMEM208 knockout HEK-293T cell line (ab266633)
Allele-1: 7 bp deletion in exon1
Sanger Sequencing - Human TMEM208 knockout HEK-293T cell line (ab266633)
Allele-3: 1 bp insertion in exon 1.
Sanger Sequencing - Human TMEM208 knockout HEK-293T cell line (ab266633)
Allele-2: 41 bp insertion in exon 1.
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