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TNF KO cell line available to order. KO validated by Western blot. Free of charge wild type control provided. Knockout achieved by using CRISPR/Cas9, Homozygous: 52 bp deletion in exon 4.

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Images

Western blot - Human TNF knockout THP-1 cell line (AB273761), expandable thumbnail
  • Western blot - Human TNF knockout THP-1 cell line (AB273761), expandable thumbnail
  • Western blot - Human TNF knockout THP-1 cell line (AB273761), expandable thumbnail
  • Sandwich ELISA - Human TNF knockout THP-1 cell line (AB273761), expandable thumbnail
  • Sanger Sequencing - Human TNF knockout THP-1 cell line (AB273761), expandable thumbnail

Key facts

Cell type
THP-1
Species or organism
Human
Tissue
Blood
Form
Liquid
Knockout validation
Sanger Sequencing, Western blot
Mutation description
Knockout achieved by using CRISPR/Cas9, Homozygous: 52 bp deletion in exon 4

Alternative names

APC1 protein, Cachectin, DIF, Differentiation inducing factor, Macrophage cytotoxic factor, TNF superfamily member 2, TNF, macrophage derived, TNF, monocyte derived, TNF-alpha, TNFA_HUMAN, TNFSF2, Tnf, Tumor Necrosis Factor, Membrane Form, Tumor necrosis factor, Tumor necrosis factor (TNF superfamily member 2), Tumor necrosis factor alpha, Tumor necrosis factor ligand superfamily member 2, Tumor necrosis factor, soluble form

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TNF KO cell line available to order. KO validated by Western blot. Free of charge wild type control provided. Knockout achieved by using CRISPR/Cas9, Homozygous: 52 bp deletion in exon 4.

Key facts

Cell type
THP-1
Form
Liquid
Mutation description
Knockout achieved by using CRISPR/Cas9, Homozygous: 52 bp deletion in exon 4
Disease
Acute Monocytic Leukemia
Concentration
Loading...

Properties

Gene name
TNF
Gene editing type
Knockout
Gene editing method
CRISPR technology
Knockout validation
Sanger Sequencing, Western blot
Zygosity
Homozygous

Quality control

STR analysis
CSF1PO, D13S317, D7S820, D5S818, TH01, D16S539, TPOX

Cell culture

Biosafety level
EU: 1 US: 1
Adherent/suspension
Suspension
Gender
Male

Handling procedures

Initial handling guidelines

Upon arrival, the vial should be stored in liquid nitrogen vapor phase and not at -80°C. Storage at -80°C may result in loss of viability.

1. Thaw the vial in 37°C water bath for approximately 1-2 minutes.
2. Transfer the cell suspension (0.8 mL) to a 15 mL/50 mL conical sterile polypropylene centrifuge tube containing 8.4 mL pre-warmed culture medium, wash vial with an additional 0.8 mL culture medium (total volume 10 mL) to collect remaining cells, and centrifuge at 201 x g (rcf) for 5 minutes at room temperature. 10 mL represents minimum recommended dilution. 20 mL represents maximum recommended dilution.
3. Resuspend the cell pellet in 5 mL pre-warmed culture medium and count using a haemocytometer or alternative cell counting method. Based on cell count, seed cells in an appropriate cell culture flask at a density of 4x105 cells/mL. Seeding density is given as a guide only and should be scaled to align with individual lab schedules.
4. Incubate the culture at 37°C incubator with 5% CO2. Cultures should be monitored daily.
5. THP-1 cells recover slowly from cryopreservation and therefore may not be ready for subculture for a number of days. Cells should be left as much as possible over this time and only subcultured when the cell density reaches 8x105 cells/mL.Small amounts of fresh media can be added until cell number/viability improves.

Subculture guidelines
  • All seeding densities should be based on cell counts gained by established methods.
  • Cells should be seeded at 2x105 - 3x105 cells/mL and subcultured when they have reached 8x105 cells/mL.
  • It is not recommended to allow the cell density to exceed 1x106 cells/mL.
Culture medium
RPMI + 10% FBS + 0.05 mM beta-mercaptoethanol
Cryopreservation medium
Cell Freezing Medium-DMSO Serum free media, contains 8.7% DMSO in MEM supplemented with methyl cellulose.

Storage

Shipped at conditions
Dry Ice
Appropriate short-term storage conditions
-196°C
Appropriate long-term storage conditions
-196°C

Notes

Recommended control: Human wild-type THP-1 cell line (ab275477). Please note a wild-type cell line is not automatically included with a knockout cell line order, if required please add recommended wild-type cell line at no additional cost using the code WILDTYPE-TMTK1.
  • Upon thawing make banks as soon as possible and use each bank within 10-20 passages
  • As cell line growth can vary please attempt culture for 2 weeks from revival of initial bank before contacting the technical team.

    • We will provide viable cells that proliferate on revival.

    This product is subject to limited use licenses from The Broad Institute and ERS Genomics Limited, and is developed with patented technology. For full details of the limited use licenses and relevant patents please refer to our limited use license and patent pages.

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    For this product, it's our understanding that no specific protocols are required. You can:

    Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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