TNK1 KO cell line available to order. Free of charge wild type control provided. Knockout achieved by using CRISPR/Cas9, 1 bp deletion in exon 2 and Insertion of the selection cassette in exon 2.
Images
Key facts
- Cell type
- HeLa
- Species or organism
- Human
- Tissue
- Cervix
- Form
- Liquid
- Knockout validation
- Sanger Sequencing
- Mutation description
- Knockout achieved by using CRISPR/Cas9, 1 bp deletion in exon 2 and Insertion of the selection cassette in exon 2
Alternative names
CD38 negative kinase 1, EC 2.7.10.2, Kinase of embryonic stem cells, Kos 1, MGC46193, Non-receptor tyrosine-protein kinase TNK1, TNK1_HUMAN, Tyrosine kinase non receptor 1
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TNK1 KO cell line available to order. Free of charge wild type control provided. Knockout achieved by using CRISPR/Cas9, 1 bp deletion in exon 2 and Insertion of the selection cassette in exon 2.
Key facts
- Cell type
- HeLa
- Species or organism
- Human
- Tissue
- Cervix
- Form
- Liquid
- Knockout validation
- Sanger Sequencing
- Mutation description
- Knockout achieved by using CRISPR/Cas9, 1 bp deletion in exon 2 and Insertion of the selection cassette in exon 2
- Disease
- Adenocarcinoma
- Concentration
Properties
- Gene name
- TNK1
- Gene editing type
- Knockout
- Gene editing method
- CRISPR technology
- Knockout validation
- Sanger Sequencing
Quality control
- STR analysis
- CSF1PO, D13S317, D7S820, D5S818, TH01, D16S539, TPOX
Cell culture
- Biosafety level
- EU: 2 US: 2
- Adherent/suspension
- Adherent
- Gender
- Female
Handling procedures
- Initial handling guidelines
Upon arrival, the vial should be stored in liquid nitrogen vapor phase and not at -80°C. Storage at -80°C may result in loss of viability.
1. Thaw the vial in 37°C water bath for approximately 1-2 minutes.
2. Transfer the cell suspension (0.8 mL) to a 15 mL/50 mL conical sterile polypropylene centrifuge tube containing 8.4 mL pre-warmed culture medium, wash vial with an additional 0.8 mL culture medium (total volume 10 mL) to collect remaining cells, and centrifuge at 201 x g (rcf) for 5 minutes at room temperature. 10 mL represents minimum recommended dilution. 20 mL represents maximum recommended dilution.
3. Resuspend the cell pellet in 5 mL pre-warmed culture medium and count using a haemocytometer or alternative cell counting method seed all remaining cells into a T25.
4. Incubate the culture at 37°C incubator with 5% CO2. Check the culture one day after revival and continue to check until 80% confluent. Media change can be given if needed.
5. Once confluent passage into an appropriate flask at a density of 2x104 cells/cm2. Seeding density is given as a guide only and should be scaled to align with individual lab schedules. Cultures should be monitored daily.- Subculture guidelines
- All seeding densities should be based on cell counts gained by established methods.
- A guide seeding density of 2x104 cells/cm2 is recommended.
- Cells should be passaged when they have achieved 80-90% confluence.
- Culture medium
- DMEM (High Glucose) + 10% FBS
- Cryopreservation medium
- Cell Freezing Medium-DMSO Serum free media, contains 8.7% DMSO in MEM supplemented with methyl cellulose.
Storage
- Shipped at conditions
- Dry Ice
- Appropriate short-term storage conditions
- -196°C
- Appropriate long-term storage conditions
- -196°C
Notes
We will provide viable cells that proliferate on revival.
This product is subject to limited use licenses from The Broad Institute, ERS Genomics Limited and Sigma-Aldrich Co. LLC, and is developed with patented technology. For full details of the licenses and patents please refer to our limited use license and patent pages.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
Tnk1 also known as Tyrosine kinase non-receptor 1 or ACK1 is a non-receptor tyrosine kinase with a molecular mass of approximately 72 kDa. This protein plays a role in signal transduction processes by phosphorylating various substrates. Tnk1 finds expression in different tissues with higher levels observed in the brain heart and testes. Its expression is vital for cellular communication and signaling cascades affecting a variety of cellular functions.
- Biological function summary
Tnk1 is involved in regulatory mechanisms that control cell growth and apoptosis. Tnk1 is not part of a well-defined complex but it interacts with various cellular proteins affected by phosphorylation. It has a role in regulating signal pathways that influence gene expression contributing to cellular responses to external stimuli. The ability of Tnk1 to modulate these processes highlights its importance in maintaining cellular homeostasis.
- Pathways
Tnk1 integrates into signaling networks such as the epidermal growth factor receptor (EGFR) pathway and Ras signaling pathway. In these pathways Tnk1 interacts with proteins like Epidermal Growth Factor Receptor (EGFR) and Ras assisting in the transduction of extracellular signals that govern cell proliferation and survival. The activity of Tnk1 within these pathways ensures that cells respond appropriately to environmental cues coordinating complex cellular responses.
- Associated diseases and disorders
Tnk1 has been implicated in various cancers including lung and breast cancer. Abnormal expression or mutations in Tnk1 may lead to deregulated cell proliferation contributing to oncogenesis. Associations with other proteins like EGFR further define its role in cancer progression. Understanding the involvement of Tnk1 in these illnesses can provide insights into potential therapeutic approaches highlighting its relevance in disease mechanisms and treatment strategies.
Product promise
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Full details and terms and conditions can be found here:
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2 product images
Sanger Sequencing - Human TNK1 knockout HeLa cell line (ab264870)
Allele-1: 1 bp deletion in exon 2.
Sanger Sequencing - Human TNK1 knockout HeLa cell line (ab264870)
Allele-2: Insertion of the selection cassette in exon 2.
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