Human TP53 (p53) knockout Jurkat cell line
- Advanced Validation
- What is this?
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TP53 KO cell line available to order. KO validated. Free of charge wild type control available. Knockout achieved by using CRISPR/Cas9, Homozygous: 46 bp deletion and 49 bp deletion in exon 4. To order both knockout and wild-type control cells: select '2 x 1000000 Cells/vial'. To order only knockout cells: select '1000000 Cells/vial'.
View Alternative Names
Antigen NY-CO-13, BCC7, Cellular tumor antigen p53, FLJ92943, LFS1, Mutant tumor protein 53, P53_HUMAN, Phosphoprotein p53, TRP53, Tp53, Transformation related protein 53, Tumor protein 53, Tumor protein p53, Tumor suppressor p53, p53 tumor suppressor, tumor antigen p55
- WB
Lab
Western blot - Human TP53 (p53) knockout Jurkat cell line (AB276112)
False colour image of Western blot : Anti-p53 antibody [DO-1] - ChIP Grade staining at 1/1000 dilution shown in green; Rabbit anti-alpha Tubulin antibody [EP1332Y] (ab52866) loading control staining at 1/20000 dilution shown in red. In Western blot ab1101 was shown to bind specifically to p53. A band was observed at 49 kDa in wild-type A549 and Jurkat cell lysates with no signal observed at this size in tp53 knockout cell lines ab276092 ab276112 (knockout cell lysates ab282999 ab283832). To generate this image wild-type and tp53 knockout A549 and Jurkat cell lysates were analysed. First samples were run on an SDS-PAGE gel then transferred onto a nitrocellulose membrane. Membranes were blocked in 3% milk in TBS-0.1 % Tween® 20 (TBS-T) before incubation with primary antibodies overnight at 4°. Blots were washed four times in TBS-T incubated with secondary antibodies for 1 h at room temperature washed again four times then imaged. Secondary antibodies used were Goat anti-Mouse IgG H&L (IRDye® 800CW) preabsorbed (ab216772) and Goat anti-Rabbit IgG H&L (IRDye® 680RD) preabsorbed (ab216777) at 1/20000 dilution.
All lanes:
Western blot - Anti-p53 antibody [DO-1] (<a href='/en-us/products/primary-antibodies/p53-antibody-do-1-chip-grade-ab1101'>ab1101</a>) at 1/1000 dilution
Lane 1:
Wild-type A549 cell lysate at 20 µg
Lane 2:
Western blot - Human TP53 (p53) knockout A549 cell line (<a href='/en-us/products/cell-lines/human-tp53-p53-knockout-a549-cell-line-ab276092'>ab276092</a>) at 20 µg
Lane 3:
Wild-type Jurkat cell lysate at 20 µg
Lane 4:
Western blot - Human TP53 (p53) knockout Jurkat cell line (ab276112) at 20 µg
Lane 5:
A431 cell lysate at 20 µg
Lane 6:
Saos-2 cell lysate at 20 µg
Predicted band size: 43 kDa
Observed band size: 49 kDa
false
- WB
Lab
Western blot - Human TP53 (p53) knockout Jurkat cell line (AB276112)
False colour image of Western blot : Anti-p53 antibody [E26] staining at 1/1000 dilution shown in green; Mouse anti-Alpha Tubulin [DM1A] (ab7291) loading control staining at 1/20000 dilution shown in red. In Western blot ab32389 was shown to bind specifically to p53. A band was observed at 49 kDa in wild-type A549 and Jurkat cell lysates with no signal observed at this size in tp53 knockout cell lines ab276092 ab276112 (knockout cell lysates ab282999 ab283832). To generate this image wild-type and tp53 knockout A549 and Jurkat cell lysates were analysed. First samples were run on an SDS-PAGE gel then transferred onto a nitrocellulose membrane. Membranes were blocked in 3% milk in TBS-0.1 % Tween® 20 (TBS-T) before incubation with primary antibodies overnight at 4°. Blots were washed four times in TBS-T incubated with secondary antibodies for 1 h at room temperature washed again four times then imaged. Secondary antibodies used were Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed (ab216776) at 1/20000 dilution.
All lanes:
Western blot - Anti-p53 antibody [E26] (<a href='/en-us/products/primary-antibodies/p53-antibody-e26-ab32389'>ab32389</a>) at 1/1000 dilution
Lane 1:
Wild-type A549 cell lysate at 20 µg
Lane 2:
TP53 knockout A549 cell lysate at 20 µg
Lane 2:
Western blot - Human TP53 (p53) knockout Jurkat cell line (ab276112)
Lane 3:
Wild-type Jurkat cell lysate at 20 µg
Lane 4:
TP53 knockout Jurkat cell lysate at 20 µg
Lane 5:
A431 cell lysate at 20 µg
Lane 6:
Saos-2 cell lysate at 20 µg
Predicted band size: 43 kDa
Observed band size: 49 kDa
false
- Sanger seq
Unknown
Sanger Sequencing - Human TP53 (p53) knockout Jurkat cell line (AB276112)
46 bp deletion and 49 bp deletion in exon 4
Reactivity data
Product details
We will provide viable cells that proliferate on revival.
Western blot data indicates that the CRISPR gene edit may have resulted in a truncation of the protein of interest. Please see data images.
This product is subject to limited use licenses from The Broad Institute and ERS Genomics Limited, and is developed with patented technology. For full details of the limited use licenses and relevant patents please refer to our limited use license and patent pages.
What's included?
Properties and storage information
Gene name
Gene editing type
Gene editing method
Knockout validation
Zygosity
Shipped at conditions
Appropriate short-term storage conditions
Appropriate long-term storage conditions
Handling procedures
Initial handling guidelines
Upon arrival, the vial should be stored in liquid nitrogen vapor phase and not at -80°C. Storage at -80°C may result in loss of viability.
1. Thaw the vial in 37°C water for bath approximately 1-2 minutes.
2. Transfer the cell suspension (0.8 mL) to a 15 mL/50 mL conical sterile polypropylene centrifuge tube containing 8.4 mL pre-warmed culture medium, wash vial with an additional 0.8 mL culture medium (total volume 10 mL) to collect remaining cells, and centrifuge at 201 x g (rcf) for 5 minutes at room temperature. 10 mL represents minimum recommended dilution. 20 mL represents maximum recommended dilution.
3. Resuspend the cell pellet in 5 mL pre-warmed culture medium and count using a haemocytometer or alternative cell counting method. Based on cell count, seed cells in an appropriate cell culture flask at a density of 2x105 cells/mL. Seeding density is given as a guide only and should be scaled to align with individual lab schedules.
4. Incubate the culture at 37°C incubator with 5% CO2. Cultures should be monitored daily.
Subculture guidelines
- All seeding densities should be based on cell counts gained by established methods.
- A guide seeding density of 2x105 cells/mL is recommended.
- Do not allow cell density to exceed 3x106 cells/mL.
Culture medium
RPMI + 10% FBS
Cryopreservation medium
Cell Freezing Medium-DMSO Serum free media, contains 8.7% DMSO in MEM supplemented with methyl cellulose.
Supplementary information
This supplementary information is collated from multiple sources and compiled automatically.
Biological function summary
P53 functions to control cell division and apoptosis serving as a guardian of the genome by preventing mutation accumulation. It does not form part of a larger complex under normal conditions but interacts with various other molecules to execute its functions. p53 can activate or suppress the transcription of numerous genes involved in cell cycle arrest DNA repair and programmed cell death allowing it to halt the progression of damaged cells and trigger repair mechanisms or eliminate those that cannot be repaired.
Pathways
P53 acts within several key biological pathways such as the p53 signaling pathway and the intrinsic apoptotic pathway. Its activity involves interaction with proteins like MDM2 which regulates p53 through ubiquitin-mediated degradation and ATM kinase which phosphorylates p53 in response to DNA damage. These interactions ensure appropriate cellular responses during stress and are vital for maintaining homeostasis.
Quality control
STR analysis
CSF1PO, D13S317, D7S820, D5S818, TH01, D16S539, TPOX
Cell culture
Biosafety level
EU: 1 US: 1
Adherent/suspension
Suspension
Gender
Male
Target data
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Product promise
Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.
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