Human TPD52L1 knockout HeLa cell line
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TPD52L1 KO cell line available to order. KO validated. Free of charge wild type control available. Knockout achieved by using CRISPR/Cas9, 7 bp deletion in exon 1 and Insertion of the selection cassette in exon 1. To order both knockout and wild-type control cells: select '2 x 1000000 Cells/vial'. To order only knockout cells: select '1000000 Cells/vial'.
View Alternative Names
D53, D54, MGC73020, MGC8556, OTTHUMP00000017133, OTTHUMP00000017136, TPD53_HUMAN, Tumor protein D52 like 2, Tumor protein D52-like 1, Tumor protein D53, Tumor protein D54, hD53, hD54
- Sanger seq
Unknown
Sanger Sequencing - Human TPD52L1 knockout HeLa cell line (AB265244)
Allele-1 : 7 bp deletion in exon 1.
- Sanger seq
Unknown
Sanger Sequencing - Human TPD52L1 knockout HeLa cell line (AB265244)
Allele-2 : Insertion of the selection cassette in exon 1.
Product details
We will provide viable cells that proliferate on revival.
This product is subject to limited use licenses from The Broad Institute, ERS Genomics Limited and Sigma-Aldrich Co. LLC, and is developed with patented technology. For full details of the licenses and patents please refer to our limited use license and patent pages.
What's included?
Properties and storage information
Gene name
Gene editing type
Gene editing method
Knockout validation
Shipped at conditions
Appropriate short-term storage conditions
Appropriate long-term storage conditions
Handling procedures
Initial handling guidelines
Upon arrival, the vial should be stored in liquid nitrogen vapor phase and not at -80°C. Storage at -80°C may result in loss of viability.
1. Thaw the vial in 37°C water bath for approximately 1-2 minutes.
2. Transfer the cell suspension (0.8 mL) to a 15 mL/50 mL conical sterile polypropylene centrifuge tube containing 8.4 mL pre-warmed culture medium, wash vial with an additional 0.8 mL culture medium (total volume 10 mL) to collect remaining cells, and centrifuge at 201 x g (rcf) for 5 minutes at room temperature. 10 mL represents minimum recommended dilution. 20 mL represents maximum recommended dilution.
3. Resuspend the cell pellet in 5 mL pre-warmed culture medium and count using a haemocytometer or alternative cell counting method seed all remaining cells into a T25.
4. Incubate the culture at 37°C incubator with 5% CO2. Check the culture one day after revival and continue to check until 80% confluent. Media change can be given if needed.
5. Once confluent passage into an appropriate flask at a density of 2x104 cells/cm2. Seeding density is given as a guide only and should be scaled to align with individual lab schedules. Cultures should be monitored daily.
Subculture guidelines
- All seeding densities should be based on cell counts gained by established methods.
- A guide seeding density of 2x104 cells/cm2 is recommended.
- Cells should be passaged when they have achieved 80-90% confluence.
Culture medium
DMEM (High Glucose) + 10% FBS
Cryopreservation medium
Cell Freezing Medium-DMSO Serum free media, contains 8.7% DMSO in MEM supplemented with methyl cellulose.
Supplementary information
This supplementary information is collated from multiple sources and compiled automatically.
Biological function summary
TPD52L1 influences cell proliferation and differentiation functions within the cell cycle. The protein does not form a complex but interacts with other proteins involved in regulating cell growth and signaling. For example it plays a role in vesicular trafficking and exocytosis which are important for maintaining cellular homeostasis. Therefore its function is important for both normal cellular functioning and response to changes in the cellular environment.
Pathways
TPD52L1 plays an active role in the PI3K/Akt signaling pathway which is critical for cell survival and proliferation. It also contributes to the ERK/MAPK pathway influencing gene transcription and cellular responses to growth signals. Within these pathways TPD52L1 interacts with proteins like Akt1 and MAPK which are pivotal in regulating cellular processes such as metabolism growth and apoptosis. Its role within these pathways highlights its involvement in important cellular decisions.
Quality control
STR analysis
CSF1PO, D13S317, D7S820, D5S818, TH01, D16S539, TPOX
Cell culture
Biosafety level
EU: 2 US: 2
Adherent/suspension
Adherent
Gender
Female
Target data
Complementary Products
Product promise
Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.
For licensing inquiries, please contact partnerships@abcam.com