TRAP1 KO cell line available to order. KO validated by Next Generation Sequencing. Free of charge wild type control provided.
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Key facts
- Cell type
- A549
- Species or organism
- Human
- Tissue
- Lung
- Form
- Liquid
- Knockout validation
- Next Generation Sequencing
Alternative names
HSP 75, HSP90L, Heat shock protein 75 kDa, Heat shock protein 75 kDa, mitochondrial, TNF receptor associated protein 1, TNFR-associated protein 1, TRAP1_HUMAN, Tumor necrosis factor type 1 receptor-associated protein, mitochondrial
Recommended products
TRAP1 KO cell line available to order. KO validated by Next Generation Sequencing. Free of charge wild type control provided.
Key facts
- Cell type
- A549
- Species or organism
- Human
- Tissue
- Lung
- Form
- Liquid
- Knockout validation
- Next Generation Sequencing
- Disease
- Carcinoma
- Concentration
Properties
- Gene name
- TRAP1
- Gene editing type
- Knockout
- Gene editing method
- CRISPR technology
- Knockout validation
- Next Generation Sequencing
Cell culture
- Biosafety level
- EU: 1 US: 1
- Adherent/suspension
- Adherent
- Gender
- Male
Handling procedures
- Initial handling guidelines
Upon arrival, the vial should be stored in liquid nitrogen vapor phase and not at -80°C. Storage at -80°C may result in loss of viability.
1. Thaw the vial in 37°C water bath for approximately 1-2 minutes.
2. Transfer the cell suspension (0.8 mL) to a 15 mL/50 mL conical sterile polypropylene centrifuge tube containing 8.4 mL pre-warmed culture medium, wash vial with an additional 0.8 mL culture medium (total volume 10 mL) to collect remaining cells, and centrifuge at 201 x g (rcf) for 5 minutes at room temperature. 10 mL represents minimum recommended dilution. 20 mL represents maximum recommended dilution.
3. Resuspend the cell pellet in 5 mL pre-warmed culture medium and count using a haemocytometer or alternative cell counting method seed all remaining cells into a T25.
4. Incubate the culture at 37°C incubator with 5% CO2. Check the culture one day after revival and continue to check until 80% confluent. Media change can be given if needed.
5. Once confluent passage into an appropriate flask at a density of 2x104 cells/cm2. Seeding density is given as a guide only and should be scaled to align with individual lab schedules. Cultures should be monitored daily.- Subculture guidelines
- All seeding densities should be based on cell counts gained by established methods.
- A guide seeding density of 2x104 cells/cm2 is recommended.
- Cells should be passaged when they have achieved 80-90% confluence.
- Do not allow the cell density to exceed 7x104 cells/cm2.
- Cryopreservation medium
- Cell Freezing Medium-DMSO Serum free media, contains 8.7% DMSO in MEM supplemented with methyl cellulose.
Storage
- Shipped at conditions
- Dry Ice
- Appropriate short-term storage conditions
- -196°C
- Appropriate long-term storage conditions
- -196°C
Notes
Recommended control: Human wild-type A549 cell line (ab288558). Please note a wild-type cell line is not automatically included with a knockout cell line order, if required please add recommended wild-type cell line at no additional cost using the code WILDTYPE-TMTK1.
We will provide viable cells that proliferate on revival.
This product is subject to limited use licenses from The Broad Institute and ERS Genomics Limited, and is developed with patented technology. For full details of the limited use licenses and relevant patents please refer to our limited use license and patent pages.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
TRAP1 also known as Tumor Necrosis Factor Receptor-Associated Protein 1 or Heat Shock Protein 75 (Hsp75) is a mitochondrial chaperone protein with a molecular weight of approximately 75 kDa. It functions to protect cells from stress-induced damage by ensuring proper protein folding and preventing aggregation within the mitochondria. TRAP1 is expressed across various tissues with higher levels observed in metabolically active organs such as the heart and liver. Its role extends to regulation of mitochondrial respiration and reactive oxygen species indicating its broad involvement in cellular homeostasis.
- Biological function summary
This mitochondrial chaperone plays an important role in maintaining protein homeostasis within the cellular environment. It forms part of a complex with heat shock proteins coordinating cellular defense mechanisms against stress. TRAP1 interacts with other chaperones to stabilize proteins and assist in mitochondrial quality control. Its activities aid in reducing oxidative stress managing apoptosis and regulating mitochondrial dynamics. Through these functions TRAP1 supports cellular survival and metabolic adaptation under conditions of stress.
- Pathways
TRAP1 is involved in the regulation of the mitochondrial apoptosis pathway and the oxidative stress response pathway. It interacts with proteins like cytochrome c and inhibitors of apoptosis proteins (IAPs) influencing the release of pro-apoptotic factors and modulation of survival signals. By modulating these pathways TRAP1 affects cellular energy production and stress responses impacting both cellular health and disease states.
- Associated diseases and disorders
TRAP1 shows a direct link to cancer and neurodegenerative diseases. Overexpression of TRAP1 has been associated with various cancers where it promotes cell survival and resistance to apoptosis. In cancer cells TRAP1 interacts with proteins like p53 and Hsp90 contributing to the oncogenic process. In neurodegenerative disorders dysfunction of TRAP1 is linked to increased oxidative stress and mitochondrial dysfunction often relating to proteins such as PINK1 and Parkin which are important in the pathology of Parkinson’s disease.
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1 product image
Next Generation Sequencing - Human TRAP1 knockout A549 cell line (ab301281)
52 bp deletion after Phe201 (allele 1), 1 bp insertion and 14 bp deletion after Gln200 (allele 2) of the WT protein
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