TRIP13 KO cell line available to order. Free of charge wild type control provided. Knockout achieved by using CRISPR/Cas9, Homozygous: Insertion of the selection cassette in exon 1.
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Key facts
- Cell type
- HEK-293T
- Species or organism
- Human
- Tissue
- Kidney
- Form
- Liquid
- Knockout validation
- Sanger Sequencing
- Mutation description
- Knockout achieved by using CRISPR/Cas9, Homozygous: Insertion of the selection cassette in exon 1
Alternative names
16E1-BP, HPV16 E1 protein-binding protein, Homo sapiens HPV16 E1 protein binding protein mRNA complete cds, Human papillomavirus type 16 E1 protein-binding protein, Pachytene checkpoint protein 2 homolog, TR-interacting protein 13, TRIP-13, TRP13_HUMAN, Thyroid hormone receptor interactor 13, Thyroid receptor-interacting protein 13
Recommended products
TRIP13 KO cell line available to order. Free of charge wild type control provided. Knockout achieved by using CRISPR/Cas9, Homozygous: Insertion of the selection cassette in exon 1.
Key facts
- Cell type
- HEK-293T
- Species or organism
- Human
- Tissue
- Kidney
- Form
- Liquid
- Knockout validation
- Sanger Sequencing
- Mutation description
- Knockout achieved by using CRISPR/Cas9, Homozygous: Insertion of the selection cassette in exon 1
- Concentration
Properties
- Gene name
- TRIP13
- Gene editing type
- Knockout
- Gene editing method
- CRISPR technology
- Knockout validation
- Sanger Sequencing
- Zygosity
- Homozygous
Quality control
- STR analysis
- CSF1PO, D13S317, D7S820, D5S818, TH01, D16S539, TPOX
Cell culture
- Biosafety level
- EU: 2 US: 2
- Adherent/suspension
- Adherent
- Gender
- Female
Handling procedures
- Initial handling guidelines
Upon arrival, the vial should be stored in liquid nitrogen vapor phase and not at -80°C. Storage at -80°C may result in loss of viability.
1. Thaw the vial in 37°C water bath for approximately 1-2 minutes.
2. Transfer the cell suspension (0.8 mL) to a 15 mL/50 mL conical sterile polypropylene centrifuge tube containing 8.4 mL pre-warmed culture medium, wash vial with an additional 0.8 mL culture medium (total volume 10 mL) to collect remaining cells, and centrifuge at 201 x g (rcf) for 5 minutes at room temperature. 10 mL represents minimum recommended dilution. 20 mL represents maximum recommended dilution.
3. Resuspend the cell pellet in 5 mL pre-warmed culture medium and count using a haemocytometer or alternative cell counting method seed all remaining cells into a T25.
4. Incubate the culture at 37°C incubator with 5% CO2. Check the culture one day after revival and continue to check until 80% confluent. Media change can be given if needed.
5. Once confluent passage into an appropriate flask at a density of 2x104 cells/cm2. Seeding density is given as a guide only and should be scaled to align with individual lab schedules. Cultures should be monitored daily.- Subculture guidelines
- All seeding densities should be based on cell counts gained by established methods.
- A guide seeding density of 2x104 cells/cm2 is recommended.
- Cells should be passaged when they have achieved 80-90% confluence.
- Culture medium
- DMEM (High Glucose) + 10% FBS
- Cryopreservation medium
- Cell Freezing Medium-DMSO Serum free media, contains 8.7% DMSO in MEM supplemented with methyl cellulose.
Storage
- Shipped at conditions
- Dry Ice
- Appropriate short-term storage conditions
- -196°C
- Appropriate long-term storage conditions
- -196°C
Notes
We will provide viable cells that proliferate on revival.
This product is subject to limited use licenses from The Broad Institute, ERS Genomics Limited and Sigma-Aldrich Co. LLC, and is developed with patented technology. For full details of the licenses and patents please refer to our limited use license and patent pages.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
TRIP13 also known as PCH2 is a protein involved in the regulation of chromosome dynamics during meiosis and mitosis. It is approximately 48 kDa in size. TRIP13 plays an important role in promoting the disassembly of the synaptonemal complex and ensuring proper chromosome segregation. This protein is expressed in various tissues but shows higher levels in testis suggesting its importance in reproductive cell division.
- Biological function summary
TRIP13 contributes to efficient cell cycle progression by participating in the spindle assembly checkpoint (SAC). It forms part of the mitotic checkpoint complex ensuring that chromosomes are correctly attached to the spindle microtubules before progression through mitosis. TRIP13 works in conjunction with other proteins like MAD2 and BUB1 to maintain genomic stability.
- Pathways
TRIP13 is involved in the cell cycle control and DNA repair pathways. It influences the mitotic spindle checkpoint interacting with proteins such as MAD2 which ensures that cells do not proceed to anaphase until all chromosomes reach proper alignment. TRIP13 also plays a role in homologous recombination repair by regulating the processing of recombination intermediates.
- Associated diseases and disorders
TRIP13 has been implicated in cancer and infertility. Its overexpression or mutation has been linked to tumor progression and resistance to certain chemotherapeutic agents due to its role in chromosome segregation and genomic stability. Additionally TRIP13 dysfunction may contribute to infertility through its impact on meiosis with potential interactions with proteins involved in reproductive processes such as SYCP3.
Product promise
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In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
1 product image
Sanger Sequencing - Human TRIP13 (PCH2) knockout HEK-293T cell line (ab266496)
Homozygous: Insertion of the selection cassette in exon 1
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