EIPR1 KO cell line available to order. Free of charge wild type control provided. Knockout achieved by using CRISPR/Cas9, 1 bp insertion in exon 4 and Insertion of the selection cassette in exon 4.
Images
Key facts
- Cell type
- HeLa
- Species or organism
- Human
- Tissue
- Cervix
- Form
- Liquid
- Knockout validation
- Sanger Sequencing
- Mutation description
- Knockout achieved by using CRISPR/Cas9, 1 bp insertion in exon 4 and Insertion of the selection cassette in exon 4
Alternative names
Tumor suppressing STF cDNA 1 protein, Tumor suppressing subchromosomal transferable fragment, Tumor suppressing subchromosomal transferable fragment candidate gene 1 protein, Tumor suppressing subtransferable candidate 1
Recommended products
EIPR1 KO cell line available to order. Free of charge wild type control provided. Knockout achieved by using CRISPR/Cas9, 1 bp insertion in exon 4 and Insertion of the selection cassette in exon 4.
Key facts
- Cell type
- HeLa
- Species or organism
- Human
- Tissue
- Cervix
- Form
- Liquid
- Knockout validation
- Sanger Sequencing
- Mutation description
- Knockout achieved by using CRISPR/Cas9, 1 bp insertion in exon 4 and Insertion of the selection cassette in exon 4
- Disease
- Adenocarcinoma
- Concentration
Properties
- Gene name
- EIPR1
- Gene editing type
- Knockout
- Gene editing method
- CRISPR technology
- Knockout validation
- Sanger Sequencing
Quality control
- STR analysis
- CSF1PO, D13S317, D7S820, D5S818, TH01, D16S539, TPOX
Cell culture
- Biosafety level
- EU: 2 US: 2
- Adherent/suspension
- Adherent
- Gender
- Female
Handling procedures
- Initial handling guidelines
Upon arrival, the vial should be stored in liquid nitrogen vapor phase and not at -80°C. Storage at -80°C may result in loss of viability.
1. Thaw the vial in 37°C water bath for approximately 1-2 minutes.
2. Transfer the cell suspension (0.8 mL) to a 15 mL/50 mL conical sterile polypropylene centrifuge tube containing 8.4 mL pre-warmed culture medium, wash vial with an additional 0.8 mL culture medium (total volume 10 mL) to collect remaining cells, and centrifuge at 201 x g (rcf) for 5 minutes at room temperature. 10 mL represents minimum recommended dilution. 20 mL represents maximum recommended dilution.
3. Resuspend the cell pellet in 5 mL pre-warmed culture medium and count using a haemocytometer or alternative cell counting method seed all remaining cells into a T25.
4. Incubate the culture at 37°C incubator with 5% CO2. Check the culture one day after revival and continue to check until 80% confluent. Media change can be given if needed.
5. Once confluent passage into an appropriate flask at a density of 2x104 cells/cm2. Seeding density is given as a guide only and should be scaled to align with individual lab schedules. Cultures should be monitored daily.- Subculture guidelines
- All seeding densities should be based on cell counts gained by established methods.
- A guide seeding density of 2x104 cells/cm2 is recommended.
- Cells should be passaged when they have achieved 80-90% confluence.
- Culture medium
- DMEM (High Glucose) + 10% FBS
- Cryopreservation medium
- Cell Freezing Medium-DMSO Serum free media, contains 8.7% DMSO in MEM supplemented with methyl cellulose.
Storage
- Shipped at conditions
- Dry Ice
- Appropriate short-term storage conditions
- -196°C
- Appropriate long-term storage conditions
- -196°C
Notes
We will provide viable cells that proliferate on revival.
This product is subject to limited use licenses from The Broad Institute, ERS Genomics Limited and Sigma-Aldrich Co. LLC, and is developed with patented technology. For full details of the licenses and patents please refer to our limited use license and patent pages.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
TSSC1 also known as Tumor Suppressor Candidate 1 plays a role in cellular machinery by facilitating protein complex formation. This protein has a molecular mass of approximately 47 kDa. Researchers find its expression in a wide range of tissues notably in highly metabolic active tissues. TSSC1's function involves maintaining structural integrity within the cell by aiding the assembly of multi-protein complexes which is essential for proper cellular operations.
- Biological function summary
TSSC1 contributes to cellular homeostasis and development. It is part of the Commander complex which is important for endosomal sorting and trafficking. By aiding the assembly and stabilization of this complex TSSC1 supports cellular processes that rely on proper endosomal transport. This function maintains cellular activities that involve the movement and sorting of various biomolecules impacting growth and response mechanisms within cells.
- Pathways
TSSC1 engages in intracellular transport and signal transduction pathways. It plays a significant role in the endosomal sorting pathway which controls material transport within cells. TSSC1 works closely with proteins like VPS26 and COMMD proteins which are essential players in endosomal sorting complexes. These interactions ensure efficient intracellular communication and materials handling affecting signal transduction pathways that mediate responses to external and internal signals.
- Associated diseases and disorders
Mutations and dysregulation of TSSC1 link to certain developmental disorders. Aberrant functioning of the endosomal sorting and trafficking process can lead to neurodevelopmental disorders due to its important role in neuronal growth and maintenance. Additionally TSSC1 may relate to cancer where its interaction with tumor suppressor pathways is altered. Disruptions in associated pathways or with proteins like TRIM25 which is involved in cellular stress responses can contribute to cancer development due to loss of regulatory control over cell proliferation.
Product promise
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In the unlikely event of one of our products not working as expected, you are covered by our product promise.
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2 product images
Sanger Sequencing - Human TSSC1 knockout HeLa cell line (ab265955)
Allele-1: 1 bp insertion in exon 4.
Sanger Sequencing - Human TSSC1 knockout HeLa cell line (ab265955)
Allele-2: Insertion of the selection cassette in exon 4.
Downloads
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