Human TUBB4B knockout HEK-293T cell line
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TUBB4B KO cell line available to order. KO validated. Free of charge wild type control available. Knockout achieved by using CRISPR/Cas9, Homozygous: 1 bp insertion in exon 3. To order both knockout and wild-type control cells: select '2 x 1000000 Cells/vial'. To order only knockout cells: select '1000000 Cells/vial'.
View Alternative Names
Beta2, Class II beta tubulin isotype, Class IVb beta tubulin, DKFZp566F223, FLJ98847, M(beta)2, MGC8685, OTTHUMP00000015956, OTTHUMP00000015964, TBB4B_HUMAN, TUBB, TUBB 2, TUBB 2A, TUBB 2C, TUBB PARALOG, TUBB2B, Tubb4b, Tubulin beta 2, Tubulin beta 2A, Tubulin beta 2A class IIa, Tubulin beta 2B, Tubulin beta 2B class IIb, Tubulin beta 2C, Tubulin beta polypeptide, Tubulin beta polypeptide 2, Tubulin beta polypeptide paralog, Tubulin beta-2 chain, Tubulin beta-2A chain, Tubulin beta-2B chain, Tubulin beta-2C chain, Tubulin beta-4B chain, Tubulin, beta, class IVB, Tubulin, beta-4B, bA506K6.1, beta 2 tubulin, class IIa beta-tubulin, class IIb beta-tubulin, dJ40E16.7, tubulin, beta 4B class IVb
- Sanger seq
Lab
Sanger Sequencing - Human TUBB4B knockout HEK-293T cell line (AB266087)
Sequencing chromatogram displaying sequence edit in exon 3
- Sanger seq
Unknown
Sanger Sequencing - Human TUBB4B knockout HEK-293T cell line (AB266087)
Homozygous : 1 bp insertion in exon 3
Product details
We will provide viable cells that proliferate on revival.
This product is subject to limited use licenses from The Broad Institute, ERS Genomics Limited and Sigma-Aldrich Co. LLC, and is developed with patented technology. For full details of the licenses and patents please refer to our limited use license and patent pages.
What's included?
Properties and storage information
Gene name
Gene editing type
Gene editing method
Knockout validation
Zygosity
Shipped at conditions
Appropriate short-term storage conditions
Appropriate long-term storage conditions
Handling procedures
Initial handling guidelines
Upon arrival, the vial should be stored in liquid nitrogen vapor phase and not at -80°C. Storage at -80°C may result in loss of viability.
1. Thaw the vial in 37°C water bath for approximately 1-2 minutes.
2. Transfer the cell suspension (0.8 mL) to a 15 mL/50 mL conical sterile polypropylene centrifuge tube containing 8.4 mL pre-warmed culture medium, wash vial with an additional 0.8 mL culture medium (total volume 10 mL) to collect remaining cells, and centrifuge at 201 x g (rcf) for 5 minutes at room temperature. 10 mL represents minimum recommended dilution. 20 mL represents maximum recommended dilution.
3. Resuspend the cell pellet in 5 mL pre-warmed culture medium and count using a haemocytometer or alternative cell counting method seed all remaining cells into a T25.
4. Incubate the culture at 37°C incubator with 5% CO2. Check the culture one day after revival and continue to check until 80% confluent. Media change can be given if needed.
5. Once confluent passage into an appropriate flask at a density of 2x104 cells/cm2. Seeding density is given as a guide only and should be scaled to align with individual lab schedules. Cultures should be monitored daily.
Subculture guidelines
- All seeding densities should be based on cell counts gained by established methods.
- A guide seeding density of 2x104 cells/cm2 is recommended.
- Cells should be passaged when they have achieved 80-90% confluence.
Culture medium
DMEM (High Glucose) + 10% FBS
Cryopreservation medium
Cell Freezing Medium-DMSO Serum free media, contains 8.7% DMSO in MEM supplemented with methyl cellulose.
Supplementary information
This supplementary information is collated from multiple sources and compiled automatically.
Biological function summary
Beta II tubulin plays an important role in cellular processes like cell division and intracellular transport. As part of the microtubule complex it provides critical support in mitotic spindle formation facilitating chromosomal segregation during mitosis. It also assists in maintaining neuronal cell stability which is vital for proper neurological functions. The dynamic nature of microtubules aided by beta II tubulin ensures the correct distribution of organelles and other intracellular structures highlighting its importance in cellular mechanics.
Pathways
Beta II tubulin interacts with several critical cellular pathways including the mitotic spindle checkpoint pathway and axonal guidance. It closely associates with other tubulins such as alpha and gamma tubulin ensuring the regulation and stabilization of microtubule dynamics. These pathways are integral to cell cycle regulation and neural development highlighting beta II tubulin's involvement in important cellular and developmental processes.
Quality control
STR analysis
CSF1PO, D13S317, D7S820, D5S818, TH01, D16S539, TPOX
Cell culture
Biosafety level
EU: 2 US: 2
Adherent/suspension
Adherent
Gender
Female
Target data
Complementary Products
Primary Antibodies
AB179513
Anti-beta Tubulin antibody [EPR16774] - Loading Control
RabMAb|Recombinant|Lab Essentials
![Flow Cytometry (Intracellular) - Anti-beta Tubulin antibody [EPR16774] - Loading Control (AB179513)](https://content.abcam.com/products/images/beta-tubulin-antibody-epr16774-loading-control-ab179513--flow-cytometry-intracellular-img117650.jpg)
- 5
- 2
Product promise
Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.
For licensing inquiries, please contact partnerships@abcam.com