TUBB4B KO cell line available to order. Free of charge wild type control provided. Knockout achieved by using CRISPR/Cas9, Homozygous: 1 bp insertion in exon 3.
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Key facts
- Cell type
- HEK-293T
- Species or organism
- Human
- Tissue
- Kidney
- Form
- Liquid
- Knockout validation
- Sanger Sequencing
- Mutation description
- Knockout achieved by using CRISPR/Cas9, Homozygous: 1 bp insertion in exon 3
Alternative names
Beta2, Class II beta tubulin isotype, Class IVb beta tubulin, DKFZp566F223, FLJ98847, M(beta)2, MGC8685, OTTHUMP00000015956, OTTHUMP00000015964, TBB4B_HUMAN, TUBB, TUBB 2, TUBB 2A, TUBB 2C, TUBB PARALOG, TUBB2B, Tubb4b, Tubulin beta 2, Tubulin beta 2A, Tubulin beta 2A class IIa, Tubulin beta 2B, Tubulin beta 2B class IIb, Tubulin beta 2C, Tubulin beta polypeptide, Tubulin beta polypeptide 2, Tubulin beta polypeptide paralog, Tubulin beta-2 chain, Tubulin beta-2A chain, Tubulin beta-2B chain, Tubulin beta-2C chain, Tubulin beta-4B chain, Tubulin, beta, class IVB, Tubulin, beta-4B, bA506K6.1, beta 2 tubulin, class IIa beta-tubulin, class IIb beta-tubulin, dJ40E16.7, tubulin, beta 4B class IVb
Recommended products
TUBB4B KO cell line available to order. Free of charge wild type control provided. Knockout achieved by using CRISPR/Cas9, Homozygous: 1 bp insertion in exon 3.
Key facts
- Cell type
- HEK-293T
- Species or organism
- Human
- Tissue
- Kidney
- Form
- Liquid
- Knockout validation
- Sanger Sequencing
- Mutation description
- Knockout achieved by using CRISPR/Cas9, Homozygous: 1 bp insertion in exon 3
- Concentration
Properties
- Gene name
- TUBB4B
- Gene editing type
- Knockout
- Gene editing method
- CRISPR technology
- Knockout validation
- Sanger Sequencing
- Zygosity
- Homozygous
Quality control
- STR analysis
- CSF1PO, D13S317, D7S820, D5S818, TH01, D16S539, TPOX
Cell culture
- Biosafety level
- EU: 2 US: 2
- Adherent/suspension
- Adherent
- Gender
- Female
Handling procedures
- Initial handling guidelines
Upon arrival, the vial should be stored in liquid nitrogen vapor phase and not at -80°C. Storage at -80°C may result in loss of viability.
1. Thaw the vial in 37°C water bath for approximately 1-2 minutes.
2. Transfer the cell suspension (0.8 mL) to a 15 mL/50 mL conical sterile polypropylene centrifuge tube containing 8.4 mL pre-warmed culture medium, wash vial with an additional 0.8 mL culture medium (total volume 10 mL) to collect remaining cells, and centrifuge at 201 x g (rcf) for 5 minutes at room temperature. 10 mL represents minimum recommended dilution. 20 mL represents maximum recommended dilution.
3. Resuspend the cell pellet in 5 mL pre-warmed culture medium and count using a haemocytometer or alternative cell counting method seed all remaining cells into a T25.
4. Incubate the culture at 37°C incubator with 5% CO2. Check the culture one day after revival and continue to check until 80% confluent. Media change can be given if needed.
5. Once confluent passage into an appropriate flask at a density of 2x104 cells/cm2. Seeding density is given as a guide only and should be scaled to align with individual lab schedules. Cultures should be monitored daily.- Subculture guidelines
- All seeding densities should be based on cell counts gained by established methods.
- A guide seeding density of 2x104 cells/cm2 is recommended.
- Cells should be passaged when they have achieved 80-90% confluence.
- Culture medium
- DMEM (High Glucose) + 10% FBS
- Cryopreservation medium
- Cell Freezing Medium-DMSO Serum free media, contains 8.7% DMSO in MEM supplemented with methyl cellulose.
Storage
- Shipped at conditions
- Dry Ice
- Appropriate short-term storage conditions
- -196°C
- Appropriate long-term storage conditions
- -196°C
Notes
We will provide viable cells that proliferate on revival.
This product is subject to limited use licenses from The Broad Institute, ERS Genomics Limited and Sigma-Aldrich Co. LLC, and is developed with patented technology. For full details of the licenses and patents please refer to our limited use license and patent pages.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
Beta II tubulin also known as beta 2 tubulin is a significant component of microtubules which are essential for maintaining cell structure and integrity. This protein with an approximate molecular mass of 50 kDa is encoded by the TUBB2B gene. It is expressed in various tissues with high expression levels in neuronal tissues contributing to the formation of the cytoskeleton by polymerizing with alpha tubulin to form heterodimers. Alternative names for this protein include 7b9 and C#7b9 though beta II is a common reference in scientific literature.
- Biological function summary
Beta II tubulin plays an important role in cellular processes like cell division and intracellular transport. As part of the microtubule complex it provides critical support in mitotic spindle formation facilitating chromosomal segregation during mitosis. It also assists in maintaining neuronal cell stability which is vital for proper neurological functions. The dynamic nature of microtubules aided by beta II tubulin ensures the correct distribution of organelles and other intracellular structures highlighting its importance in cellular mechanics.
- Pathways
Beta II tubulin interacts with several critical cellular pathways including the mitotic spindle checkpoint pathway and axonal guidance. It closely associates with other tubulins such as alpha and gamma tubulin ensuring the regulation and stabilization of microtubule dynamics. These pathways are integral to cell cycle regulation and neural development highlighting beta II tubulin's involvement in important cellular and developmental processes.
- Associated diseases and disorders
Mutations or dysregulation in beta II tubulin can relate to neurological disorders such as malformation of cortical development and certain types of brain atrophy. These are often linked to the protein's key role in neuronal function and axonal transport. Additionally altered expression of beta II tubulin has a connection to microtubule-related proteins such as tau which are implicated in neurodegenerative disorders like Alzheimer's disease. This highlights the importance of beta II tubulin in maintaining neuronal health and provides potential targets for therapeutic interventions.
Product promise
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2 product images
Sanger Sequencing - Human TUBB4B knockout HEK-293T cell line (ab266087)
Homozygous: 1 bp insertion in exon 3
Sanger Sequencing - Human TUBB4B knockout HEK-293T cell line (ab266087)
Sequencing chromatogram displaying sequence edit in exon 3
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