Human TXNDC5 knockout HEK-293T cell line
- Advanced Validation
- What is this?
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TXNDC5 KO cell line available to order. KO validated by Western blot. Free of charge wild type control provided. Knockout achieved by using CRISPR/Cas9, Homozygous: 1 bp insertion in exon 2.
View Alternative Names
ER protein 46, ERp46, EndoPDI, Endoplasmic reticulum protein ERp46, Endoplasmic reticulum resident protein 46, Endothelial protein disulphide isomerase, HCC-2, MGC3178, PDIA15, Protein disulfide isomerase family A member 15, STRF8, TLP46, TXND5_HUMAN, Thioredoxin domain containing 5, Thioredoxin domain containing 5 (endoplasmic reticulum), Thioredoxin domain-containing protein 5, Thioredoxin related protein, Thioredoxin-like protein p46
- WB
Lab
Western blot - Human TXNDC5 knockout HEK-293T cell line (AB266609)
False colour image of Western blot : Anti-TXNDC5 antibody staining at 0.1 μg/ml shown in green; Mouse anti-GAPDH antibody [6C5] (ab8245) loading control staining at 1/20000 dilution shown in red. In Western blot ab13820 was shown to bind specifically to TXNDC5. A band was observed at 47 kDa in wild-type HEK-293T cell lysates with no signal observed at this size in TXNDC5 knockout cell line ab266609 (knockout cell lysate ab263403). To generate this image wild-type and TXNDC5 knockout HEK-293T cell lysates were analysed. First samples were run on an SDS-PAGE gel then transferred onto a nitrocellulose membrane. Membranes were blocked in 3 % milk in TBS-0.1 % Tween® 20 (TBS-T) before incubation with primary antibodies overnight at 4 °. Blots were washed four times in TBS-T incubated with secondary antibodies for 1 h at room temperature washed again four times then imaged.Secondary antibodies used were Donkey anti-Goat IgG H&L (IRDye® 800CW) preabsorbed (ab216775) and Donkey anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed (ab216778) at 1/20000 dilution.
All lanes:
Western blot - Anti-TXNDC5 antibody (<a href='/en-us/products/primary-antibodies/txndc5-antibody-ab13820'>ab13820</a>) at 0.1 µg/mL
Lane 1:
Wild-type HEK-293T cell lysate at 20 µg
Lane 2:
TXNDC5 knockout HEK-293T cell lysate at 20 µg
Lane 2:
Western blot - Human TXNDC5 knockout HEK-293T cell line (ab266609)
Lane 3:
HeLa cell lysate at 20 µg
Lane 4:
A549 cell lysate at 20 µg
Predicted band size: 48 kDa
Observed band size: 47 kDa
false
- Sanger seq
Unknown
Sanger Sequencing - Human TXNDC5 knockout HEK-293T cell line (AB266609)
Homozygous : 1 bp insertion in exon 2
- Cell Culture
Lab
Cell Culture - Human TXNDC5 knockout HEK-293T cell line (AB266609)
Representative images TXNDC5 knockout HEK293T cells, low and high confluency examples (top left and right respectively) and wild-type HEK293T cells, low and high confluency (bottom left and right respectively) showing typical adherent, epithelial-like morphology. Images were captured at 10X magnification using a EVOS M5000 microscope.
Reactivity data
Product details
Recommended control: Human wild-type HEK293T cell line (ab255449). Please note a wild-type cell line is not automatically included with a knockout cell line order, if required please add recommended wild-type cell line at no additional cost using the code WILDTYPE-TMTK1.
We will provide viable cells that proliferate on revival.
This product is subject to limited use licenses from The Broad Institute, ERS Genomics Limited and Sigma-Aldrich Co. LLC, and is developed with patented technology. For full details of the licenses and patents please refer to our limited use license and patent pages.
What's included?
Properties and storage information
Gene name
Gene editing type
Gene editing method
Knockout validation
Zygosity
Shipped at conditions
Appropriate short-term storage conditions
Appropriate long-term storage conditions
Handling procedures
Initial handling guidelines
Upon arrival, the vial should be stored in liquid nitrogen vapor phase and not at -80°C. Storage at -80°C may result in loss of viability.
1. Thaw the vial in 37°C water bath for approximately 1-2 minutes.
2. Transfer the cell suspension (0.8 mL) to a 15 mL/50 mL conical sterile polypropylene centrifuge tube containing 8.4 mL pre-warmed culture medium, wash vial with an additional 0.8 mL culture medium (total volume 10 mL) to collect remaining cells, and centrifuge at 201 x g (rcf) for 5 minutes at room temperature. 10 mL represents minimum recommended dilution. 20 mL represents maximum recommended dilution.
3. Resuspend the cell pellet in 5 mL pre-warmed culture medium and count using a haemocytometer or alternative cell counting method seed all remaining cells into a T25.
4. Incubate the culture at 37°C incubator with 5% CO2. Check the culture one day after revival and continue to check until 80% confluent. Media change can be given if needed.
5. Once confluent passage into an appropriate flask at a density of 2x104 cells/cm2. Seeding density is given as a guide only and should be scaled to align with individual lab schedules. Cultures should be monitored daily.
Subculture guidelines
- All seeding densities should be based on cell counts gained by established methods.
- A guide seeding density of 2x104 cells/cm2 is recommended.
- Cells should be passaged when they have achieved 80-90% confluence.
Culture medium
DMEM (High Glucose) + 10% FBS
Cryopreservation medium
Cell Freezing Medium-DMSO Serum free media, contains 8.7% DMSO in MEM supplemented with methyl cellulose.
Supplementary information
This supplementary information is collated from multiple sources and compiled automatically.
Biological function summary
TXNDC5 contributes to the oxidative folding of proteins within the endoplasmic reticulum. The protein does not act alone but as part of a larger complex involving other chaperones and foldases that together ensure protein quality control. TXNDC5 interfaces with the redox system impacting the oxidative environment necessary for correct protein folding. By contributing to this complex system TXNDC5 helps prevent the accumulation of misfolded proteins and maintains the integrity of cellular function.
Pathways
TXNDC5 plays a role in the protein folding pathway which is vital to maintaining cellular stability. In addition its involvement in the response to unfolded protein responses signifies its importance in stress-related pathways. TXNDC5 often interacts with other proteins such as PDI (Protein Disulfide Isomerase) and Calreticulin which are also important in protein folding and cellular stress responses. These pathways are essential for adapting to changes in protein synthesis demands and mitigating the effects of cellular stress.
Quality control
STR analysis
CSF1PO, D13S317, D7S820, D5S818, TH01, D16S539, TPOX
Cell culture
Biosafety level
EU: 2 US: 2
Adherent/suspension
Adherent
Gender
Female
Target data
Complementary Products
Product promise
Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.
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