Human UBE2D4 knockout HeLa cell line
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- Sanger seq
Unknown
Sanger Sequencing - Human UBE2D4 knockout HeLa cell line (AB265326)
Homozygous : 1 bp insertion in exon 2.
- Sanger seq
Lab
Sanger Sequencing - Human UBE2D4 knockout HeLa cell line (AB265326)
Sequencing chromatogram displaying sequence edit in exon 2
Complementary Products
Product details
We will provide viable cells that proliferate on revival.
This product is subject to limited use licenses from The Broad Institute, ERS Genomics Limited and Sigma-Aldrich Co. LLC, and is developed with patented technology. For full details of the licenses and patents please refer to our limited use license and patent pages.
What's included?
Properties and storage information
Gene name
Gene editing type
Gene editing method
Knockout validation
Zygosity
Shipped at conditions
Appropriate short-term storage conditions
Appropriate long-term storage conditions
Handling procedures
Initial handling guidelines
Upon arrival, the vial should be stored in liquid nitrogen vapor phase and not at -80°C. Storage at -80°C may result in loss of viability.
1. Thaw the vial in 37°C water bath for approximately 1-2 minutes.
2. Transfer the cell suspension (0.8 mL) to a 15 mL/50 mL conical sterile polypropylene centrifuge tube containing 8.4 mL pre-warmed culture medium, wash vial with an additional 0.8 mL culture medium (total volume 10 mL) to collect remaining cells, and centrifuge at 201 x g (rcf) for 5 minutes at room temperature. 10 mL represents minimum recommended dilution. 20 mL represents maximum recommended dilution.
3. Resuspend the cell pellet in 5 mL pre-warmed culture medium and count using a haemocytometer or alternative cell counting method seed all remaining cells into a T25.
4. Incubate the culture at 37°C incubator with 5% CO2. Check the culture one day after revival and continue to check until 80% confluent. Media change can be given if needed.
5. Once confluent passage into an appropriate flask at a density of 2x104 cells/cm2. Seeding density is given as a guide only and should be scaled to align with individual lab schedules. Cultures should be monitored daily.
Subculture guidelines
- All seeding densities should be based on cell counts gained by established methods.
- A guide seeding density of 2x104 cells/cm2 is recommended.
- Cells should be passaged when they have achieved 80-90% confluence.
Culture medium
DMEM (High Glucose) + 10% FBS
Cryopreservation medium
Cell Freezing Medium-DMSO Serum free media, contains 8.7% DMSO in MEM supplemented with methyl cellulose.
Supplementary information
This supplementary information is collated from multiple sources and compiled automatically.
Biological function summary
The ubiquitin-conjugating enzyme plays a role in protein homeostasis by facilitating the ubiquitin-proteasome system a complex that includes E1 E2 and E3 enzymes. UBE2D4 acts in concert with specific E3 ligases to regulate cell cycle progression and transcriptional regulation. This process ensures the removal of misfolded or damaged proteins thereby maintaining protein quality within cells. Its involvement in diverse cellular pathways highlights the enzyme's importance in controlling cellular activities.
Pathways
UBE2D4 operates in the ubiquitin-mediated proteolysis and cell cycle regulatory pathways. These pathways control protein turnover and degradation ensuring cellular equilibrium. UBE2D4 collaborates with E3 ligases like MDM2 within these pathways influencing proteins like p53 which is important for cell cycle control and apoptosis. The interconnectedness with MDM2 emphasizes UBE2D4's role in key cellular events.
Quality control
STR analysis
CSF1PO, D13S317, D7S820, D5S818, TH01, D16S539, TPOX
Cell culture
Biosafety level
EU: 2 US: 2
Adherent/suspension
Adherent
Gender
Female
Target data
Product promise
Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.
For licensing inquiries, please contact partnerships@abcam.com