UBE2H KO cell line available to order. Free of charge wild type control provided. Knockout achieved by using CRISPR/Cas9, 2 bp deletion in exon 1 and 8 bp deletion in exon 1.
Images
Key facts
- Cell type
- HeLa
- Species or organism
- Human
- Tissue
- Cervix
- Form
- Liquid
- Knockout validation
- Sanger Sequencing
- Mutation description
- Knockout achieved by using CRISPR/Cas9, 2 bp deletion in exon 1 and 8 bp deletion in exon 1
Alternative names
E2 20K, GID complex subunit 3 UBC8 homolog, GID3, UBC8, UBCH, UBCH 2, UBE2H_HUMAN, Ubiquitin carrier protein H, Ubiquitin conjugating enzyme E2H (UBC8 homolog yeast), Ubiquitin-conjugating enzyme E2 H, Ubiquitin-conjugating enzyme E2-20K, Ubiquitin-conjugating enzyme E2H (homologous to yeast UBC8), Ubiquitin-protein ligase H
Recommended products
UBE2H KO cell line available to order. Free of charge wild type control provided. Knockout achieved by using CRISPR/Cas9, 2 bp deletion in exon 1 and 8 bp deletion in exon 1.
Key facts
- Cell type
- HeLa
- Species or organism
- Human
- Tissue
- Cervix
- Form
- Liquid
- Knockout validation
- Sanger Sequencing
- Mutation description
- Knockout achieved by using CRISPR/Cas9, 2 bp deletion in exon 1 and 8 bp deletion in exon 1
- Disease
- Adenocarcinoma
- Concentration
Properties
- Gene name
- UBE2H
- Gene editing type
- Knockout
- Gene editing method
- CRISPR technology
- Knockout validation
- Sanger Sequencing
Quality control
- STR analysis
- CSF1PO, D13S317, D7S820, D5S818, TH01, D16S539, TPOX
Cell culture
- Biosafety level
- EU: 2 US: 2
- Adherent/suspension
- Adherent
- Gender
- Female
Handling procedures
- Initial handling guidelines
Upon arrival, the vial should be stored in liquid nitrogen vapor phase and not at -80°C. Storage at -80°C may result in loss of viability.
1. Thaw the vial in 37°C water bath for approximately 1-2 minutes.
2. Transfer the cell suspension (0.8 mL) to a 15 mL/50 mL conical sterile polypropylene centrifuge tube containing 8.4 mL pre-warmed culture medium, wash vial with an additional 0.8 mL culture medium (total volume 10 mL) to collect remaining cells, and centrifuge at 201 x g (rcf) for 5 minutes at room temperature. 10 mL represents minimum recommended dilution. 20 mL represents maximum recommended dilution.
3. Resuspend the cell pellet in 5 mL pre-warmed culture medium and count using a haemocytometer or alternative cell counting method seed all remaining cells into a T25.
4. Incubate the culture at 37°C incubator with 5% CO2. Check the culture one day after revival and continue to check until 80% confluent. Media change can be given if needed.
5. Once confluent passage into an appropriate flask at a density of 2x104 cells/cm2. Seeding density is given as a guide only and should be scaled to align with individual lab schedules. Cultures should be monitored daily.- Subculture guidelines
- All seeding densities should be based on cell counts gained by established methods.
- A guide seeding density of 2x104 cells/cm2 is recommended.
- Cells should be passaged when they have achieved 80-90% confluence.
- Culture medium
- DMEM (High Glucose) + 10% FBS
- Cryopreservation medium
- Cell Freezing Medium-DMSO Serum free media, contains 8.7% DMSO in MEM supplemented with methyl cellulose.
Storage
- Shipped at conditions
- Dry Ice
- Appropriate short-term storage conditions
- -196°C
- Appropriate long-term storage conditions
- -196°C
Notes
We will provide viable cells that proliferate on revival.
This product is subject to limited use licenses from The Broad Institute and ERS Genomics Limited, and is developed with patented technology. For full details of the limited use licenses and relevant patents please refer to our limited use license and patent pages.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
Ubiquitin-conjugating enzyme E2 H (Ube2H) also known as UBC8 is an enzyme that functions mechanically to facilitate the transfer of ubiquitin to substrate proteins a process essential for regulating protein degradation. Ube2H weighs approximately 20 kDa and shows expression in various tissues especially in the liver and skeletal muscle. It belongs to the E2 ubiquitin-conjugating enzyme family which plays an important role in the ubiquitin-proteasome pathway.
- Biological function summary
Ubiquitin-conjugating enzyme E2 H plays a central role in protein homeostasis by tagging proteins for proteasomal degradation. It does not function alone but as part of larger complexes that include E1 activating enzymes and E3 ubiquitin ligases. These complexes work in synchrony to ensure precise ubiquitination of target proteins which helps regulate multiple cellular processes such as cell cycle progression DNA repair and immune response.
- Pathways
Ubiquitin-conjugating enzyme E2 H integrates with several critical cellular pathways including the ubiquitin-proteasome pathway and the DNA damage response pathway. In the ubiquitin-proteasome pathway Ube2H works alongside other E2 enzymes like Ube2C to assist in the ubiquitination process. It has a notable connection with proteins like p53 and BRCA1 which are vital in maintaining genomic stability and tumor suppression.
- Associated diseases and disorders
Ubiquitin-conjugating enzyme E2 H has recognized implications in cancer and neurodegenerative diseases. Aberrations in its function can lead to improper protein degradation contributing to the accumulation of defective proteins that can cause cellular dysfunction. In cancer Ube2H is often linked with anomalies in the ubiquitin-proteasome system affecting the degradation of oncogenic proteins. Additionally its interaction with p53 a protein known for its tumor suppressor functions highlights its potential involvement in cancer progression when dysregulated.
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3 product images
Sanger Sequencing - Human UBE2H knockout HeLa cell line (ab265455)
Allele-1: 8 bp deletion in exon 1.
Sanger Sequencing - Human UBE2H knockout HeLa cell line (ab265455)
Allele-2: 2 bp deletion in exon 1.
Sanger Sequencing - Human UBE2H knockout HeLa cell line (ab265455)
Sequencing chromatogram displaying sequence edit in exon 1
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