UBE2O KO cell line available to order. Free of charge wild type control provided. Knockout achieved by using CRISPR/Cas9, 1 bp insertion in exon 8 and 2 bp deletion in exon 8.
Images
Key facts
- Cell type
- HeLa
- Species or organism
- Human
- Tissue
- Cervix
- Form
- Liquid
- Knockout validation
- Sanger Sequencing
- Mutation description
- Knockout achieved by using CRISPR/Cas9, 1 bp insertion in exon 8 and 2 bp deletion in exon 8
Alternative names
E2 230K, EC=6.3.2.19, FLJ12878, KIAA1734, Likely Ortholog of Mouse Ubiquitin Conjugating Enzyme, RP23 193A16.5, UBE2O_HUMAN, Ubiquitin carrier protein O, Ubiquitin-conjugating enzyme E2 O, Ubiquitin-conjugating enzyme E2 of 230 kDa, Ubiquitin-conjugating enzyme E2-230K, Ubiquitin-protein ligase O
Recommended products
UBE2O KO cell line available to order. Free of charge wild type control provided. Knockout achieved by using CRISPR/Cas9, 1 bp insertion in exon 8 and 2 bp deletion in exon 8.
Key facts
- Cell type
- HeLa
- Species or organism
- Human
- Tissue
- Cervix
- Form
- Liquid
- Knockout validation
- Sanger Sequencing
- Mutation description
- Knockout achieved by using CRISPR/Cas9, 1 bp insertion in exon 8 and 2 bp deletion in exon 8
- Disease
- Adenocarcinoma
- Concentration
Properties
- Gene name
- UBE2O
- Gene editing type
- Knockout
- Gene editing method
- CRISPR technology
- Knockout validation
- Sanger Sequencing
Quality control
- STR analysis
- CSF1PO, D13S317, D7S820, D5S818, TH01, D16S539, TPOX
Cell culture
- Biosafety level
- EU: 2 US: 2
- Adherent/suspension
- Adherent
- Gender
- Female
Handling procedures
- Initial handling guidelines
Upon arrival, the vial should be stored in liquid nitrogen vapor phase and not at -80°C. Storage at -80°C may result in loss of viability.
1. Thaw the vial in 37°C water bath for approximately 1-2 minutes.
2. Transfer the cell suspension (0.8 mL) to a 15 mL/50 mL conical sterile polypropylene centrifuge tube containing 8.4 mL pre-warmed culture medium, wash vial with an additional 0.8 mL culture medium (total volume 10 mL) to collect remaining cells, and centrifuge at 201 x g (rcf) for 5 minutes at room temperature. 10 mL represents minimum recommended dilution. 20 mL represents maximum recommended dilution.
3. Resuspend the cell pellet in 5 mL pre-warmed culture medium and count using a haemocytometer or alternative cell counting method seed all remaining cells into a T25.
4. Incubate the culture at 37°C incubator with 5% CO2. Check the culture one day after revival and continue to check until 80% confluent. Media change can be given if needed.
5. Once confluent passage into an appropriate flask at a density of 2x104 cells/cm2. Seeding density is given as a guide only and should be scaled to align with individual lab schedules. Cultures should be monitored daily.- Subculture guidelines
- All seeding densities should be based on cell counts gained by established methods.
- A guide seeding density of 2x104 cells/cm2 is recommended.
- Cells should be passaged when they have achieved 80-90% confluence.
- Culture medium
- DMEM (High Glucose) + 10% FBS
- Cryopreservation medium
- Cell Freezing Medium-DMSO Serum free media, contains 8.7% DMSO in MEM supplemented with methyl cellulose.
Storage
- Shipped at conditions
- Dry Ice
- Appropriate short-term storage conditions
- -196°C
- Appropriate long-term storage conditions
- -196°C
Notes
We will provide viable cells that proliferate on revival.
This product is subject to limited use licenses from The Broad Institute, ERS Genomics Limited and Sigma-Aldrich Co. LLC, and is developed with patented technology. For full details of the licenses and patents please refer to our limited use license and patent pages.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
UBE2O also known as E2-230K or E2 ubiquitin-conjugating enzyme O is a protein that functions as an E2 ubiquitin-conjugating enzyme. It has a molecular mass of approximately 141 kDa. UBE2O plays a role in transferring ubiquitin to substrate proteins marking them for degradation via the proteasome. This process is important for protein quality control and cellular homeostasis. UBE2O is expressed in various tissues including the brain liver and skeletal muscle indicating its broad physiological significance.
- Biological function summary
UBE2O facilitates the regulation of protein turnover by mediating ubiquitination without the need for an E3 ligase. It acts both as an E2 and has E3 activity making it a versatile factor in the ubiquitin-proteasome system. UBE2O also participates in muscle differentiation by targeting specific muscle regulatory factors for ubiquitination. Understanding its function provides insights into the control of muscle mass and function highlighting its contribution to cellular growth and adaptation.
- Pathways
UBE2O interacts with essential biological mechanisms notably the ubiquitin-proteasome pathway involved in protein degradation. It also participates in cell cycle regulation by targeting certain cyclin proteins for degradation ensuring proper cell cycle progression. UBE2O works in conjunction with other proteins like ANAPC10 and CDC20 which are important in cell cycle control and proteostasis.
- Associated diseases and disorders
UBE2O has associations with conditions such as myopathies and certain cancers. In muscle disorders UBE2O dysregulation can lead to abnormal protein accumulation affecting muscle function. In oncology UBE2O interacts with proteins like P53 which is important in cell cycle regulation and apoptosis. Its role in these disorders underlines its importance as a potential therapeutic target for intervention in these diseases.
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3 product images
Sanger Sequencing - Human UBE2O knockout HeLa cell line (ab264948)
Allele-1: 2 bp deletion in exon 8.
Sanger Sequencing - Human UBE2O knockout HeLa cell line (ab264948)
Allele-2: 1 bp insertion in exon 8.
Sanger Sequencing - Human UBE2O knockout HeLa cell line (ab264948)
Sequencing chromatogram displaying sequence edit in exon 8
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