Human UBE3A knockout U-87 MG cell line

• WB

Lab

Western blot - Human UBE3A knockout U-87 MG cell line (AB306798)

Western blot : Rabbit Monoclonal[EPR25059-12] to UBE3A ab290641 staining at 1/1000 dilution, shown in green; Mouse anti GAPDH (ab8245) loading control staining at 1/20,000 dilution, shown in magenta. A band was observed at 100 kDa in Wild-type U-87 MG cell lysates with a truncated band observed in UBE3A knockout U-87 MG ab306798 cell line. To generate this image, samples were run on an SDS-PAGE gel then transferred onto a nitrocellulose membrane. Membranes were blocked in 3pc Milk in TBS-0.1 % Tween^® 20 (TBS-T) before incubation with primary antibodies overnight at 4 °C. Blots were washed four times in TBS-T, incubated with secondary antibodies for 1 h at room temperature, washed again four times then imaged. Secondary antibodies used were Goat anti-Rabbit 800CW & Goat anti-Mouse 680RD at 1/20,000 dilution.

All lanes:

Western blot - Anti-UBE3A antibody [EPR25059-12] (<a href='/en-us/products/primary-antibodies/ube3a-antibody-epr25059-12-ab290641'>ab290641</a>) at 1/1000 dilution

Lane 1:

Wild-type U-87 MG at 20 µg

Lane 2:

Western blot - Human UBE3A knockout U-87 MG cell line (ab306798) at 20 µg

Lane 2:

knockout U-87 MG at 20 µg

Lane 3:

Human Brain at 20 µg

Lane 4:

K562 at 20 µg

Lane 5:

T-47D at 20 µg

Secondary

Lanes 1 - 5:

Goat anti-Rabbit 800CW at 1/20000 dilution

Lanes 1 - 5:

Goat anti-Mouse 680RD at 1/20000 dilution

Predicted band size: 101 kDa

Observed band size: 100 kDa,36 kDa

false

• NGS

Lab

Next Generation Sequencing - Human UBE3A knockout U-87 MG cell line (AB306798)

62 bp deletion (allele 1), 170 bp insertion and 169 bp deletion (allele 2) in exon 3, CCDS45191.1