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UFC1 KO cell line available to order. KO validated by Western blot. Free of charge wild type control provided. Knockout achieved by using CRISPR/Cas9, Homozygous: 16 bp deletion in exon 2.

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Images

Western blot - Human UFC1 knockout HEK-293T cell line (AB266814), expandable thumbnail
  • Western blot - Human UFC1 knockout HEK-293T cell line (AB266814), expandable thumbnail
  • Sanger Sequencing - Human UFC1 knockout HEK-293T cell line (AB266814), expandable thumbnail

Key facts

Cell type
HEK-293T
Species or organism
Human
Tissue
Kidney
Form
Liquid
Knockout validation
Sanger Sequencing, Western blot
Mutation description
Knockout achieved by using CRISPR/Cas9, Homozygous: 16 bp deletion in exon 2

Alternative names

CGI-126, HSPC155, UFC1_HUMAN, Ubiquitin-fold modifier-conjugating enzyme 1, Ufm1-conjugating enzyme 1

Recommended products

UFC1 KO cell line available to order. KO validated by Western blot. Free of charge wild type control provided. Knockout achieved by using CRISPR/Cas9, Homozygous: 16 bp deletion in exon 2.

Key facts

Cell type
HEK-293T
Form
Liquid
Mutation description
Knockout achieved by using CRISPR/Cas9, Homozygous: 16 bp deletion in exon 2
Concentration
Loading...

Properties

Gene name
UFC1
Gene editing type
Knockout
Gene editing method
CRISPR technology
Knockout validation
Sanger Sequencing, Western blot
Zygosity
Homozygous

Quality control

STR analysis
CSF1PO, D13S317, D7S820, D5S818, TH01, D16S539, TPOX

Cell culture

Biosafety level
EU: 2 US: 2
Adherent/suspension
Adherent
Gender
Female

Handling procedures

Initial handling guidelines

Upon arrival, the vial should be stored in liquid nitrogen vapor phase and not at -80°C. Storage at -80°C may result in loss of viability.

1. Thaw the vial in 37°C water bath for approximately 1-2 minutes.
2. Transfer the cell suspension (0.8 mL) to a 15 mL/50 mL conical sterile polypropylene centrifuge tube containing 8.4 mL pre-warmed culture medium, wash vial with an additional 0.8 mL culture medium (total volume 10 mL) to collect remaining cells, and centrifuge at 201 x g (rcf) for 5 minutes at room temperature. 10 mL represents minimum recommended dilution. 20 mL represents maximum recommended dilution.
3. Resuspend the cell pellet in 5 mL pre-warmed culture medium and count using a haemocytometer or alternative cell counting method seed all remaining cells into a T25.
4. Incubate the culture at 37°C incubator with 5% CO2. Check the culture one day after revival and continue to check until 80% confluent. Media change can be given if needed.
5. Once confluent passage into an appropriate flask at a density of 2x104 cells/cm2. Seeding density is given as a guide only and should be scaled to align with individual lab schedules. Cultures should be monitored daily.

Subculture guidelines
  • All seeding densities should be based on cell counts gained by established methods.
  • A guide seeding density of 2x104 cells/cm2 is recommended.
  • Cells should be passaged when they have achieved 80-90% confluence.
Culture medium
DMEM (High Glucose) + 10% FBS
Cryopreservation medium
Cell Freezing Medium-DMSO Serum free media, contains 8.7% DMSO in MEM supplemented with methyl cellulose.

Storage

Shipped at conditions
Dry Ice
Appropriate short-term storage conditions
-196°C
Appropriate long-term storage conditions
-196°C

Notes

Recommended control: Human wild-type HEK293T cell line (ab255449). Please note a wild-type cell line is not automatically included with a knockout cell line order, if required please add recommended wild-type cell line at no additional cost using the code WILDTYPE-TMTK1.

We will provide viable cells that proliferate on revival.

This product is subject to limited use licenses from The Broad Institute and ERS Genomics Limited, and is developed with patented technology. For full details of the limited use licenses and relevant patents please refer to our limited use license and patent pages.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.
Activity summary

UFC1 also known as ubiquitin-fold modifier-conjugating enzyme 1 or UFM1-conjugating enzyme 1 functions mechanically by facilitating the conjugation of ubiquitin-fold modifier 1 (UFM1) to target substrates. It exhibits a molecular mass of approximately 20.8 kDa. UFC1 is expressed in a variety of tissues including liver kidney and heart indicating its widespread role in cellular processes. As an essential component in the UFM1 system UFC1 works closely with other proteins to regulate protein modification through UFMylation.

Biological function summary

UFC1 is involved in the process of UFMylation which modifies proteins through the attachment of UFM1 much like ubiquitination influences protein degradation and signaling. UFC1 forms part of a complex that also includes UBA5 and UFL1 the E1 activating enzyme and E3 ligase respectively. This UFMylation system is necessary for endoplasmic reticulum-associated degradation (ERAD) contributing to the maintenance of protein homeostasis within cells. The disruption of this modification process can impact cellular stress response and protein quality control mechanisms.

Pathways

UFC1 plays a critical role in the ER stress response and protein quality control pathways. Within these pathways UFC1 interacts with proteins such as UFM1 and UFL1 to mediate the UFMylation of target proteins during cellular stress. Additionally UFC1 has been linked to pathways regulating autophagy where it indirectly influences the degradation of defective proteins. This interaction ultimately affects the cellular response to misfolded proteins and is pivotal for maintaining cellular equilibrium.

Associated diseases and disorders

UFC1 has implications in conditions such as cancer and neurological disorders. Abnormalities in UFMylation can contribute to the dysregulation of protein homeostasis which is frequently observed in cancerous cells. In the context of neurological disorders the dysfunctional UFM1 system involving UFC1 can result in impaired neuronal homeostasis. Additionally UFC1's relationship with proteins like UFM1 and UFL1 highlights its importance in the pathological processes of these diseases positioning it as a potential target for therapeutic intervention.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

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