Human UQCRQ knockout HEK-293T cell line
- Advanced Validation
- What is this?
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(1 Publication)
UQCRQ KO cell line available to order. KO validated by. Free of charge wild type control provided. Knockout achieved by using CRISPR/Cas9, Homozygous: 1 bp deletion in exon 3.
View Alternative Names
Complex III subunit 8, Complex III subunit VIII, Cytochrome b-c1 complex subunit 8, QCR8_HUMAN, QP-C, Ubiquinol-cytochrome c reductase complex 9.5 kDa protein, Ubiquinol-cytochrome c reductase complex ubiquinone-binding protein QP-C
- WB
Lab
Western blot - Human UQCRQ knockout HEK-293T cell line (AB267244)
False colour image of Western blot : Anti-UQCRQ antibody staining at 1/1000 dilution shown in black; Mouse anti-Alpha Tubulin [DM1A] (ab7291) loading control staining at 1/20000 dilution shown in red. In Western blot ab241983 was shown to bind specifically to UQCRQ. A band was observed at 11 kDa in wild-type HEK-293T cell lysates with no signal observed at this size in Uqcrq knockout cell line ab267244 (knockout cell lysate ab258273). To generate this image wild-type and Uqcrq knockout HEK-293T cell lysates were analysed. First samples were run on an SDS-PAGE gel then transferred onto a nitrocellulose membrane. Membranes were blocked in 3 % milk in TBS-0.1 % Tween® 20 (TBS-T) before incubation with primary antibodies overnight at 4 °. Blots were washed four times in TBS-T incubated with secondary antibodies for 1 h at room temperature washed again four times before development with Optiblot (ECL reagent ab133456) and imaged with 1 minute exposure time. Secondary antibodies used were HRP conjugated Goat anti-Rabbit (H+L) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed (ab216776) at 1/20000 dilution.
All lanes:
Western blot - Anti-UQCRQ antibody (<a href='/en-us/products/primary-antibodies/uqcrq-antibody-ab241983'>ab241983</a>) at 1/1000 dilution
Lane 1:
Wild-type HEK-293T cell lysate at 20 µg
Lane 2:
Uqcrq knockout HEK-293T cell lysate at 20 µg
Lane 2:
Western blot - Human UQCRQ knockout HEK-293T cell line (ab267244)
Lane 3:
Human Skeletal muscle cell lysate at 20 µg
Lane 4:
Human Ovary cell lysate at 20 µg
Predicted band size: 10 kDa
Observed band size: 11 kDa
false
- Sanger seq
Unknown
Sanger Sequencing - Human UQCRQ knockout HEK-293T cell line (AB267244)
Homozygous : 1 bp deletion in exon3
- Cell Culture
Unknown
Cell Culture - Human UQCRQ knockout HEK-293T cell line (AB267244)
Representative images of UQCRQ knockout HEK293T cells, low and high confluency examples (top left and right respectively) and wild-type HEK293T cells, low and high confluency (bottom left and right respectively) showing typical adherent, epithelial-like morphology. Images were captured at 10X magnification using an EVOS M5000 microscope.
Reactivity data
Product details
Recommended control: Human wild-type HEK293T cell line (ab255449). Please note a wild-type cell line is not automatically included with a knockout cell line order, if required please add recommended wild-type cell line at no additional cost using the code WILDTYPE-TMTK1.
We will provide viable cells that proliferate on revival.
This product is subject to limited use licenses from The Broad Institute and ERS Genomics Limited, and is developed with patented technology. For full details of the limited use licenses and relevant patents please refer to our limited use license and patent pages.
What's included?
Properties and storage information
Gene name
Gene editing type
Gene editing method
Knockout validation
Zygosity
Shipped at conditions
Appropriate short-term storage conditions
Appropriate long-term storage conditions
Handling procedures
Initial handling guidelines
Upon arrival, the vial should be stored in liquid nitrogen vapor phase and not at -80°C. Storage at -80°C may result in loss of viability.
1. Thaw the vial in 37°C water bath for approximately 1-2 minutes.
2. Transfer the cell suspension (0.8 mL) to a 15 mL/50 mL conical sterile polypropylene centrifuge tube containing 8.4 mL pre-warmed culture medium, wash vial with an additional 0.8 mL culture medium (total volume 10 mL) to collect remaining cells, and centrifuge at 201 x g (rcf) for 5 minutes at room temperature. 10 mL represents minimum recommended dilution. 20 mL represents maximum recommended dilution.
3. Resuspend the cell pellet in 5 mL pre-warmed culture medium and count using a haemocytometer or alternative cell counting method seed all remaining cells into a T25.
4. Incubate the culture at 37°C incubator with 5% CO2. Check the culture one day after revival and continue to check until 80% confluent. Media change can be given if needed.
5. Once confluent passage into an appropriate flask at a density of 2x104 cells/cm2. Seeding density is given as a guide only and should be scaled to align with individual lab schedules. Cultures should be monitored daily.
Subculture guidelines
- All seeding densities should be based on cell counts gained by established methods.
- A guide seeding density of 2x104 cells/cm2 is recommended.
- Cells should be passaged when they have achieved 80-90% confluence.
Culture medium
DMEM (High Glucose) + 10% FBS
Cryopreservation medium
Cell Freezing Medium-DMSO Serum free media, contains 8.7% DMSO in MEM supplemented with methyl cellulose.
Supplementary information
This supplementary information is collated from multiple sources and compiled automatically.
Biological function summary
UQCRQ is a part of the cytochrome bc1 complex which is a critical enzyme in the mitochondrial inner membrane. This complex is essential in cellular respiration helping in the conversion of energy from nutrients into usable cellular energy in the form of ATP. The activity of UQCRQ is important for maintaining the efficiency of this larger complex and any disruption can significantly affect cellular energy homeostasis. UQCRQ supports the integrity of mitochondrial function impacting both energy production and reactive oxygen species management.
Pathways
UQCRQ is pivotal in oxidative phosphorylation and the electron transport chain specifically within the mitochondrial respiratory complex III. It works closely with other proteins in the complex such as cytochrome b and cytochrome c1 ensuring efficient electron transfer. Through these pathways UQCRQ contributes to oxidative stress regulation and cellular energy metabolism linking energy-producing mechanisms to broader cellular processes like apoptosis and cell growth.
Quality control
STR analysis
CSF1PO, D13S317, D7S820, D5S818, TH01, D16S539, TPOX
Cell culture
Biosafety level
EU: 2 US: 2
Adherent/suspension
Adherent
Gender
Female
Publications (1)
Recent publications for all applications. Explore the full list and refine your search
Functional & integrative genomics 25:90 PubMed40240625
2025
Applications
Unspecified application
Species
Unspecified reactive species
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Product promise
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