USP42 KO cell line available to order. Free of charge wild type control provided. Knockout achieved by using CRISPR/Cas9, 2 bp deletion in exon 2 and 5 bp deletion in exon 2 and 7 bp deletion in exon 2.
Images
Key facts
- Cell type
- HeLa
- Species or organism
- Human
- Tissue
- Cervix
- Form
- Liquid
- Knockout validation
- Sanger Sequencing
- Mutation description
- Knockout achieved by using CRISPR/Cas9, 2 bp deletion in exon 2 and 5 bp deletion in exon 2 and 7 bp deletion in exon 2
Alternative names
Deubiquitinating enzyme 42, FLJ12697, UBP42_HUMAN, Ubiquitin carboxyl-terminal hydrolase 42, Ubiquitin thiolesterase 42, Ubiquitin-specific-processing protease 42, ubiquitin specific peptidase 42
Recommended products
USP42 KO cell line available to order. Free of charge wild type control provided. Knockout achieved by using CRISPR/Cas9, 2 bp deletion in exon 2 and 5 bp deletion in exon 2 and 7 bp deletion in exon 2.
Key facts
- Cell type
- HeLa
- Species or organism
- Human
- Tissue
- Cervix
- Form
- Liquid
- Knockout validation
- Sanger Sequencing
- Mutation description
- Knockout achieved by using CRISPR/Cas9, 2 bp deletion in exon 2 and 5 bp deletion in exon 2 and 7 bp deletion in exon 2
- Disease
- Adenocarcinoma
- Concentration
Properties
- Gene name
- USP42
- Gene editing type
- Knockout
- Gene editing method
- CRISPR technology
- Knockout validation
- Sanger Sequencing
Quality control
- STR analysis
- CSF1PO, D13S317, D7S820, D5S818, TH01, D16S539, TPOX
Cell culture
- Biosafety level
- EU: 2 US: 2
- Adherent/suspension
- Adherent
- Gender
- Female
Handling procedures
- Initial handling guidelines
Upon arrival, the vial should be stored in liquid nitrogen vapor phase and not at -80°C. Storage at -80°C may result in loss of viability.
1. Thaw the vial in 37°C water bath for approximately 1-2 minutes.
2. Transfer the cell suspension (0.8 mL) to a 15 mL/50 mL conical sterile polypropylene centrifuge tube containing 8.4 mL pre-warmed culture medium, wash vial with an additional 0.8 mL culture medium (total volume 10 mL) to collect remaining cells, and centrifuge at 201 x g (rcf) for 5 minutes at room temperature. 10 mL represents minimum recommended dilution. 20 mL represents maximum recommended dilution.
3. Resuspend the cell pellet in 5 mL pre-warmed culture medium and count using a haemocytometer or alternative cell counting method seed all remaining cells into a T25.
4. Incubate the culture at 37°C incubator with 5% CO2. Check the culture one day after revival and continue to check until 80% confluent. Media change can be given if needed.
5. Once confluent passage into an appropriate flask at a density of 2x104 cells/cm2. Seeding density is given as a guide only and should be scaled to align with individual lab schedules. Cultures should be monitored daily.- Subculture guidelines
- All seeding densities should be based on cell counts gained by established methods.
- A guide seeding density of 2x104 cells/cm2 is recommended.
- Cells should be passaged when they have achieved 80-90% confluence.
- Culture medium
- DMEM (High Glucose) + 10% FBS
- Cryopreservation medium
- Cell Freezing Medium-DMSO Serum free media, contains 8.7% DMSO in MEM supplemented with methyl cellulose.
Storage
- Shipped at conditions
- Dry Ice
- Appropriate short-term storage conditions
- -196°C
- Appropriate long-term storage conditions
- -196°C
Notes
We will provide viable cells that proliferate on revival.
This product is subject to limited use licenses from The Broad Institute and ERS Genomics Limited, and is developed with patented technology. For full details of the limited use licenses and relevant patents please refer to our limited use license and patent pages.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
USP42 also known as Ubiquitin-specific peptidase 42 is part of the ubiquitin-proteasome system. USP42 specifically removes ubiquitin molecules from specific protein substrates. This action involves deubiquitination a process that regulates protein degradation. The molecular weight of USP42 is approximately 150 kDa. In terms of expression USP42 is found in various tissues including the liver brain and kidneys indicating it has a broad role across different systems of the body.
- Biological function summary
The function of USP42 is important in gene regulation and cell cycle control. By controlling protein stability it influences transcriptional activity and cellular responses to stress. USP42 associates with the p53 protein stabilizing it and enhancing its tumor suppressor function. Additionally it forms part of larger protein complexes that coordinate DNA damage responses. Through these interactions USP42 supports cellular health by ensuring proper repair and replication of DNA.
- Pathways
Four words that are not related to USP42 regulation involve the ubiquitin-proteasome and DNA damage response pathways. In these pathways USP42 plays a part in modulating ubiquitin ligases and deubiquitinases activities maintaining protein homeostasis. The protein acts in concert with others like Mdm2 and p53 important for cellular stress responses and apoptosis. These pathways highlight the impact of USP42 in balancing cell survival and programmed cell death mechanisms.
- Associated diseases and disorders
More than five words not related to USP42 include cancer and neurodegenerative diseases. Altered USP42 expression or function has links to tumorigenesis due to its interaction with proteins such as p53. Dysregulation in this pathway may contribute to cancer proliferation. In neurodegenerative conditions abnormal protein aggregation occurs if ubiquitin-proteasome function is impaired connecting to USP42's role in maintaining protein quality control. The protein's dysfunction can therefore influence disease progression by failing to regulate protein turnover properly.
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3 product images
Sanger Sequencing - Human USP42 knockout HeLa cell line (ab264981)
Allele-3: 2 bp deletion in exon 2.
Sanger Sequencing - Human USP42 knockout HeLa cell line (ab264981)
Allele-2: 5 bp deletion in exon 2.
Sanger Sequencing - Human USP42 knockout HeLa cell line (ab264981)
Allele-1: 7 bp deletion in exon 2.
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