Human USP54 knockout HeLa cell line
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USP54 KO cell line available to order. KO validated by. Free of charge wild type control provided. Knockout achieved by using CRISPR/Cas9, Homozygous: 107 bp insertion in exon 5.
View Alternative Names
C10orf29, FLJ37318, Inactive ubiquitin carboxyl-terminal hydrolase 54, bA137L10.3, bA137L10.4, nactive ubiquitin-specific peptidase 54
- Sanger seq
Unknown
Sanger Sequencing - Human USP54 knockout HeLa cell line (AB265768)
Homozygous : 107 bp insertion in exon 5.
Product details
Recommended control: Human wild-type HeLa cell line (ab255928). Please note a wild-type cell line is not automatically included with a knockout cell line order, if required please add recommended wild-type cell line at no additional cost using the code WILDTYPE-TMTK1.
We will provide viable cells that proliferate on revival.
This product is subject to limited use licenses from The Broad Institute, ERS Genomics Limited and Sigma-Aldrich Co. LLC, and is developed with patented technology. For full details of the licenses and patents please refer to our limited use license and patent pages.
What's included?
Properties and storage information
Gene name
Gene editing type
Gene editing method
Knockout validation
Zygosity
Shipped at conditions
Appropriate short-term storage conditions
Appropriate long-term storage conditions
Handling procedures
Initial handling guidelines
Upon arrival, the vial should be stored in liquid nitrogen vapor phase and not at -80°C. Storage at -80°C may result in loss of viability.
1. Thaw the vial in 37°C water bath for approximately 1-2 minutes.
2. Transfer the cell suspension (0.8 mL) to a 15 mL/50 mL conical sterile polypropylene centrifuge tube containing 8.4 mL pre-warmed culture medium, wash vial with an additional 0.8 mL culture medium (total volume 10 mL) to collect remaining cells, and centrifuge at 201 x g (rcf) for 5 minutes at room temperature. 10 mL represents minimum recommended dilution. 20 mL represents maximum recommended dilution.
3. Resuspend the cell pellet in 5 mL pre-warmed culture medium and count using a haemocytometer or alternative cell counting method seed all remaining cells into a T25.
4. Incubate the culture at 37°C incubator with 5% CO2. Check the culture one day after revival and continue to check until 80% confluent. Media change can be given if needed.
5. Once confluent passage into an appropriate flask at a density of 2x104 cells/cm2. Seeding density is given as a guide only and should be scaled to align with individual lab schedules. Cultures should be monitored daily.
Subculture guidelines
- All seeding densities should be based on cell counts gained by established methods.
- A guide seeding density of 2x104 cells/cm2 is recommended.
- Cells should be passaged when they have achieved 80-90% confluence.
Culture medium
DMEM (High Glucose) + 10% FBS
Cryopreservation medium
Cell Freezing Medium-DMSO Serum free media, contains 8.7% DMSO in MEM supplemented with methyl cellulose.
Supplementary information
This supplementary information is collated from multiple sources and compiled automatically.
Biological function summary
USP54 plays a role in modulating protein turnover and recycling through its deubiquitinating activity. This regulation helps cells maintain balance in protein levels and activity. Unlike some other deubiquitinating enzymes USP54 is not known to be a component of a larger protein complex. However it shares functional similarities with other enzymes in the ubiquitin-proteasome system which orchestrates the targeted degradation of proteins to control cellular processes.
Pathways
USP54 engages in the ubiquitin-proteasome pathway a critical pathway for protein degradation. This pathway ensures timely removal of misfolded or damaged proteins. USP54 may interact with proteins involved in this pathway to fine-tune the degradation process. Within the context of cellular regulatory networks USP54 may also have connections with signaling pathways where regulation of protein levels impacts signaling cascades. For instance proteins like UBE2D3 and UBC are ubiquitin-conjugating enzymes that might be related through shared pathway dynamics.
Quality control
STR analysis
CSF1PO, D13S317, D7S820, D5S818, TH01, D16S539, TPOX
Cell culture
Biosafety level
EU: 2 US: 2
Adherent/suspension
Adherent
Gender
Female
Target data
Complementary Products
Product promise
Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.
For licensing inquiries, please contact partnerships@abcam.com