Skip to main content

USP9X KO cell line available to order. KO validated by Western blot. Free of charge wild type control provided. Knockout achieved by using CRISPR/Cas9, 1 bp deletion in exon 9 and 1 bp insertion in exon 9.

Be the first to review this product! Submit a review

Images

Western blot - Human USP9X knockout HeLa cell line (AB265665), expandable thumbnail
  • Western blot - Human USP9X knockout HeLa cell line (AB265665), expandable thumbnail
  • Sanger Sequencing - Human USP9X knockout HeLa cell line (AB265665), expandable thumbnail
  • Sanger Sequencing - Human USP9X knockout HeLa cell line (AB265665), expandable thumbnail

Key facts

Cell type
HeLa
Species or organism
Human
Tissue
Cervix
Form
Liquid
Knockout validation
Sanger Sequencing, Western blot
Mutation description
Knockout achieved by using CRISPR/Cas9, 1 bp deletion in exon 9 and 1 bp insertion in exon 9

Alternative names

DFFRX, Deubiquitinating enzyme FAF-X, Drosophila fat facets related X linked, FAF, Fafl, Fat facets homolog, Fat facets in mammals, Fat facets protein related X linked, Fat facets protein-related, MRX99, Probable ubiquitin carboxyl-terminal hydrolase FAF-X, USP9 (gene name), USP9X_HUMAN, Ubiquitin carboxyl-terminal hydrolase FAM, Ubiquitin specific peptidase 9 X linked, Ubiquitin specific protease 9 X chromosome, Ubiquitin thioesterase FAF X, Ubiquitin thiolesterase FAF-X, Ubiquitin-specific protease 9, Ubiquitin-specific-processing protease FAF-X, Uubiquitin specific protease 9, X chromosome (fat facets like Drosophila), X chromosome, X-linked, hFAM

Recommended products

USP9X KO cell line available to order. KO validated by Western blot. Free of charge wild type control provided. Knockout achieved by using CRISPR/Cas9, 1 bp deletion in exon 9 and 1 bp insertion in exon 9.

Key facts

Cell type
HeLa
Form
Liquid
Mutation description
Knockout achieved by using CRISPR/Cas9, 1 bp deletion in exon 9 and 1 bp insertion in exon 9
Disease
Adenocarcinoma
Concentration
Loading...

Properties

Gene name
USP9X
Gene editing type
Knockout
Gene editing method
CRISPR technology
Knockout validation
Sanger Sequencing, Western blot

Quality control

STR analysis
CSF1PO, D13S317, D7S820, D5S818, TH01, D16S539, TPOX

Cell culture

Biosafety level
EU: 2 US: 2
Adherent/suspension
Adherent
Gender
Female

Handling procedures

Initial handling guidelines

Upon arrival, the vial should be stored in liquid nitrogen vapor phase and not at -80°C. Storage at -80°C may result in loss of viability.

1. Thaw the vial in 37°C water bath for approximately 1-2 minutes.
2. Transfer the cell suspension (0.8 mL) to a 15 mL/50 mL conical sterile polypropylene centrifuge tube containing 8.4 mL pre-warmed culture medium, wash vial with an additional 0.8 mL culture medium (total volume 10 mL) to collect remaining cells, and centrifuge at 201 x g (rcf) for 5 minutes at room temperature. 10 mL represents minimum recommended dilution. 20 mL represents maximum recommended dilution.
3. Resuspend the cell pellet in 5 mL pre-warmed culture medium and count using a haemocytometer or alternative cell counting method seed all remaining cells into a T25.
4. Incubate the culture at 37°C incubator with 5% CO2. Check the culture one day after revival and continue to check until 80% confluent. Media change can be given if needed.
5. Once confluent passage into an appropriate flask at a density of 2x104 cells/cm2. Seeding density is given as a guide only and should be scaled to align with individual lab schedules. Cultures should be monitored daily.

Subculture guidelines
  • All seeding densities should be based on cell counts gained by established methods.
  • A guide seeding density of 2x104 cells/cm2 is recommended.
  • Cells should be passaged when they have achieved 80-90% confluence.
Culture medium
DMEM (High Glucose) + 10% FBS
Cryopreservation medium
Cell Freezing Medium-DMSO Serum free media, contains 8.7% DMSO in MEM supplemented with methyl cellulose.

Storage

Shipped at conditions
Dry Ice
Appropriate short-term storage conditions
-196°C
Appropriate long-term storage conditions
-196°C

Notes

Recommended control: Human wild-type HeLa cell line (ab255928). Please note a wild-type cell line is not automatically included with a knockout cell line order, if required please add recommended wild-type cell line at no additional cost using the code WILDTYPE-TMTK1.

We will provide viable cells that proliferate on revival.

This product is subject to limited use licenses from The Broad Institute, ERS Genomics Limited and Sigma-Aldrich Co. LLC, and is developed with patented technology. For full details of the licenses and patents please refer to our limited use license and patent pages.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.
Activity summary

USP9x also known as ubiquitin-specific peptidase 9 X-linked is a deubiquitinating enzyme that plays a critical role in protein homeostasis. USP9x belongs to the USP family and has an approximate molecular mass of 290 kDa. It is expressed broadly in human tissues with particularly high levels in the brain and pancreas. Mechanically USP9x functions to remove ubiquitin molecules from ubiquitinated substrates preventing their degradation by the proteasome. This regulatory action influences many cellular processes by stabilizing specific proteins.

Biological function summary

USP9x influences numerous cellular mechanisms by maintaining protein balance. It is a part of the complex cellular machinery involved in signal transduction cell division and apoptosis. While its enzyme activity regulates various cellular proteins USP9x is essential in neural development and differentiation. The proteolytic activity helps to modulate pathways by controlling protein turnover which impacts cellular responses and development. Failure to regulate proteins accurately can lead to compromised cellular functions.

Pathways

USP9x participates prominently in the Wnt signaling and TGF-beta pathways. Its role in deubiquitination allows it to stabilize proteins like beta-catenin and SMAD4 which are vital for transmitting signals within these pathways. Through these interactions USP9x aids in cell proliferation migration and differentiation often connecting with other proteins like APC or Axin in the Wnt pathway. This demonstrates how USP9x's enzymatic activity intricately links to key biological signaling mechanisms.

Associated diseases and disorders

Mutations and dysregulation of USP9x associate with cancer and neurodevelopmental disorders. In cancer it impairs normal cell cycle control and apoptosis facilitating unchecked cell proliferation. It interacts with proteins such as MCL1 influencing apoptosis decisions and tumor growth. In neurodevelopmental disorders USP9x affects brain development by disrupting neural cell differentiation processes. The protein's malfunction causes imbalances in brain signaling linking it to conditions like intellectual disability.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

4 product images

Downloads

Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com