VDAC3 KO cell line available to order. Free of charge wild type control provided. Knockout achieved by using CRISPR/Cas9, 16 bp deletion in exon 6 and 1 bp insertion in exon 6.
Images
Key facts
- Cell type
- HEK-293T
- Species or organism
- Human
- Tissue
- Kidney
- Form
- Liquid
- Knockout validation
- Sanger Sequencing
- Mutation description
- Knockout achieved by using CRISPR/Cas9, 16 bp deletion in exon 6 and 1 bp insertion in exon 6
Alternative names
Outer mitochondrial membrane protein porin 3, hVDAC3, mVDAC3, voltage dependent anion channel 3
Recommended products
VDAC3 KO cell line available to order. Free of charge wild type control provided. Knockout achieved by using CRISPR/Cas9, 16 bp deletion in exon 6 and 1 bp insertion in exon 6.
Key facts
- Cell type
- HEK-293T
- Species or organism
- Human
- Tissue
- Kidney
- Form
- Liquid
- Knockout validation
- Sanger Sequencing
- Mutation description
- Knockout achieved by using CRISPR/Cas9, 16 bp deletion in exon 6 and 1 bp insertion in exon 6
- Concentration
Properties
- Gene name
- VDAC3
- Gene editing type
- Knockout
- Gene editing method
- CRISPR technology
- Knockout validation
- Sanger Sequencing
Quality control
- STR analysis
- CSF1PO, D13S317, D7S820, D5S818, TH01, D16S539, TPOX
Cell culture
- Biosafety level
- EU: 2 US: 2
- Adherent/suspension
- Adherent
- Gender
- Female
Handling procedures
- Initial handling guidelines
Upon arrival, the vial should be stored in liquid nitrogen vapor phase and not at -80°C. Storage at -80°C may result in loss of viability.
1. Thaw the vial in 37°C water bath for approximately 1-2 minutes.
2. Transfer the cell suspension (0.8 mL) to a 15 mL/50 mL conical sterile polypropylene centrifuge tube containing 8.4 mL pre-warmed culture medium, wash vial with an additional 0.8 mL culture medium (total volume 10 mL) to collect remaining cells, and centrifuge at 201 x g (rcf) for 5 minutes at room temperature. 10 mL represents minimum recommended dilution. 20 mL represents maximum recommended dilution.
3. Resuspend the cell pellet in 5 mL pre-warmed culture medium and count using a haemocytometer or alternative cell counting method seed all remaining cells into a T25.
4. Incubate the culture at 37°C incubator with 5% CO2. Check the culture one day after revival and continue to check until 80% confluent. Media change can be given if needed.
5. Once confluent passage into an appropriate flask at a density of 2x104 cells/cm2. Seeding density is given as a guide only and should be scaled to align with individual lab schedules. Cultures should be monitored daily.- Subculture guidelines
- All seeding densities should be based on cell counts gained by established methods.
- A guide seeding density of 2x104 cells/cm2 is recommended.
- Cells should be passaged when they have achieved 80-90% confluence.
- Culture medium
- DMEM (High Glucose) + 10% FBS
- Cryopreservation medium
- Cell Freezing Medium-DMSO Serum free media, contains 8.7% DMSO in MEM supplemented with methyl cellulose.
Storage
- Shipped at conditions
- Dry Ice
- Appropriate short-term storage conditions
- -196°C
- Appropriate long-term storage conditions
- -196°C
Notes
We will provide viable cells that proliferate on revival.
This product is subject to limited use licenses from The Broad Institute, ERS Genomics Limited and Sigma-Aldrich Co. LLC, and is developed with patented technology. For full details of the licenses and patents please refer to our limited use license and patent pages.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
Voltage-dependent anion channel 3 (VDAC3) also known as porin 3 has a molecular mass of approximately 32 kDa. It is a part of the voltage-dependent anion channel family located in the outer mitochondrial membrane. VDAC3 plays a role in regulating the passage of ions and metabolites across the mitochondrial membrane. This channel shares a similar structure with other VDAC isoforms and is expressed in several tissues with high levels found in the heart and skeletal muscle.
- Biological function summary
VDAC3 contributes to cellular energy homeostasis and the exchange of ions and small metabolites between mitochondria and cytoplasm. VDAC3 may participate in a complex with other mitochondrial proteins affecting the mitochondrial permeability transition pore (mPTP). The precise regulation of VDAC3 ensures normal mitochondrial function which is critical for maintaining cellular metabolism and programming cell death including apoptotic pathways.
- Pathways
VDAC3 is involved in apoptotic regulation and energy metabolism. Its activity impacts the intrinsic pathway of apoptosis where it interacts with Bcl-2 family proteins such as Bax and Bak influencing cytochrome c release. VDAC3's role in these pathways connects it to overall cellular stress responses and energy balance. Additionally VDAC3 coordinates with other VDAC isoforms to ensure mitochondrial integrity under variable physiological conditions.
- Associated diseases and disorders
VDAC3's malfunction links to neurodegenerative diseases and cardiac disorders. Dysfunction in VDAC3 has associations with diseases like Alzheimer's due to its involvement in mitochondrial dysfunction. Additionally its influence on apoptotic pathways implicates VDAC3 in heart diseases where improper apoptosis can lead to cardiac tissue damage. In these contexts VDAC3 interacts with the protein amyloid-beta in Alzheimer's and with caspases in cardiac conditions suggesting its importance in disease mechanisms.
Product promise
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2 product images
Sanger Sequencing - Human VDAC3 knockout HEK-293T cell line (ab266066)
Allele-1: 16 bp deletion in exon 6
Sanger Sequencing - Human VDAC3 knockout HEK-293T cell line (ab266066)
Allele-2: 1 bp insertion in exon 6.
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