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AB306807

Human VLDLR knockout U-87 MG cell line

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VLDLR KO cell line available to order. KO validated by Next Generation Sequencing, Western blot. Free of charge wild type control available. knockout. To order both knockout and wild-type control cells: select '2 x 1000000 Cells/vial'. To order only knockout cells: select '1000000 Cells/vial'.
2 Images
Western blot - Human VLDLR knockout U-87 MG cell line (AB306807)
  • WB

Lab

Western blot - Human VLDLR knockout U-87 MG cell line (AB306807)

Western blot : Rabbit Monoclonal [EPR26178-72] to VLDL Receptor/VLDL-R ab302917 staining at 1/1000 dilution, shown in green; Mouse anti GAPDH (ab8245) loading control staining at 1/20,000 dilution, shown in magenta.

A band was observed at 115/140 kDa in Wild-type U-87 MG UNBOILED cell lysates with no signal observed at this size in VLDLR knockout U-87 MG UNBOILED cell line.

To generate this image, samples were run on an SDS-PAGE gel then transferred onto a nitrocellulose membrane. Membranes were blocked in 3pc Milk in TBS-0.1 % Tween® 20 (TBS-T) before incubation with primary antibodies overnight at 4 °C. Blots were washed four times in TBS-T, incubated with secondary antibodies for 1 h at room temperature, washed again four times then imaged.

Secondary antibodies used were Goat anti-Rabbit 800CW & Goat anti-Mouse 680RD at 1/20,000 dilution.

All lanes:

Western blot - Anti-VLDL Receptor/VLDL-R antibody [EPR26178-72] (<a href='/en-us/products/primary-antibodies/vldl-receptor-vldl-r-antibody-epr26178-72-ab302917'>ab302917</a>) at 1/1000 dilution

Lane 1:

Wild-type U-87 MG UNBOILED at 20 µg

Lane 2:

VLDLR knockout U-87 MG UNBOILED at 20 µg

Lane 2:

Western blot - Human VLDLR knockout U-87 MG cell line (ab306807) at 20 µg

Lane 3:

THP-1 UNBOILED at 20 µg

Lane 4:

Daudi UNBOILED at 20 µg

Secondary

All lanes:

Goat anti-Rabbit 800CW & Goat anti-Mouse 680RD at 1/20000 dilution

Predicted band size: 96 kDa

Observed band size: 115 kDa,140 kDa

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Next Generation Sequencing - Human VLDLR knockout U-87 MG cell line (AB306807)
  • NGS

Lab

Next Generation Sequencing - Human VLDLR knockout U-87 MG cell line (AB306807)

172 bp deletion after Asn99 of the WT protein

Key facts

Cell type

U-87 MG

Species or organism

Human

Tissue

Brain

Form

Liquid

form

Knockout validation

Next Generation Sequencing,Western blot

Mutation description

knockout

Disease

Glioblastoma

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Complementary Products

Primary Antibodies

AB302917

Anti-VLDL Receptor/VLDL-R antibody [EPR26178-72]

BOND RX™ Validated|20ul selling size|RabMAb|Recombinant|KO Validated

Immunohistochemistry - Anti-VLDL Receptor/VLDL-R antibody [EPR26178-72] (AB302917)

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "NGS": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Product details

Although we aim to provide customers with a homozygous clone, feasibility will be dependent on the biology of the protein. Should only heterozygous edits be achieved, you will be notified of the outcome and be asked to confirm whether the cell line is acceptable. All clones will be accompanied with DNA sequencing data, and the mutation description.

This product is subject to limited use licenses from The Broad Institute and ERS Genomics Limited, and is developed with patented technology. For full details of the limited use licenses and relevant patents please refer to our limited use license and patent pages.

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What's included?

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Properties and storage information

Gene name
VLDLR
Gene editing type
Knockout
Gene editing method
CRISPR technology
Knockout validation
Next Generation Sequencing, Western blot
Shipped at conditions
Dry Ice
Appropriate short-term storage conditions
-196°C
Appropriate long-term storage conditions
-196°C
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Handling procedures

Initial handling guidelines
  • All seeding densities should be based on cell counts gained by established methods.
  • A guide seeding density of 2x104 cells/cm2 is recommended.
  • Cells should be passaged when they have achieved 80-90% confluence.
Subculture guidelines
  • All seeding densities should be based on cell counts gained by established methods.
  • A guide seeding density of 2x104 cells/cm2 is recommended.
  • Cells should be passaged when they have achieved 80-90% confluence.
Culture medium

EMEM + 10% FBS

Cryopreservation medium

Cell Freezing Medium-DMSO Serum free media, contains 8.7% DMSO in MEM supplemented with methyl cellulose.

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The VLDL Receptor also known as VLDL-R mediates the uptake of very low-density lipoproteins (VLDLs) into cells. This receptor has a molecular mass of approximately 104 kDa. It is structurally similar to the low-density lipoprotein (LDL) receptor and binds to apolipoprotein E-containing lipoproteins. VLDL-R expresses in several tissues including heart skeletal muscle and adipose tissue. Its distribution suggests an important role in lipid metabolism for energy production and storage.
Biological function summary

VLDL-R significantly influences lipid and lipoprotein metabolism by facilitating the endocytosis of triglyceride-rich particles. It functions within the larger receptor family known as the LDL receptor family. VLDL-R plays an important role in cellular lipid uptake and the subsequent supply of fatty acids which are necessary for various metabolic processes. This receptor does not function as part of a protein complex but its interactions with other proteins can alter its activity and the cell's metabolic state.

Pathways

This receptor influences lipid metabolism and energy homeostasis pathways. It directly impacts the lipid biosynthetic pathway by mediating the cellular uptake of lipid components necessary for these processes. The receptor interacts with proteins like apolipoprotein B and E which regulate lipoprotein assembly and clearance. In this pathway VLDL-R works conjunctionally with the LDL receptor to maintain lipid balance within the body.

VLDL-R relates to metabolic syndrome and obesity. Abnormalities in VLDL-R function can lead to dyslipidemia a risk factor for these conditions due to the mishandling of lipid uptake. This association connects VLDL-R to apolipoprotein E and B whose mutations can also contribute to these metabolic disorders. Understanding how VLDL-R operates in these contexts may provide insight into therapeutic targets for these widespread health issues.
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Cell culture

Biosafety level

EU: 1 US: 1

Adherent/suspension

Adherent

Gender

Male

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Product protocols

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Target data

See full target information VLDLR
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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