Human WDR34 knockout HEK-293T cell line
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DYNC2I2 KO cell line available to order. KO validated by. Free of charge wild type control provided. Knockout achieved by using CRISPR/Cas9, 1 bp deletion in exon 1 and 5 bp deletion in exon 1.
View Alternative Names
DIC5, MGC20486, OTTHUMP00000022282, RP11 216B9.5, WD repeat domain 34, WD repeat-containing protein 34, WDR34_HUMAN, bA216B9.3
- Sanger seq
Unknown
Sanger Sequencing - Human WDR34 knockout HEK-293T cell line (AB266593)
Allele-1 : 1 bp deletion in exon1
- Sanger seq
Unknown
Sanger Sequencing - Human WDR34 knockout HEK-293T cell line (AB266593)
Allele-2 : 5 bp deletion in exon 1.
Product details
Recommended control: Human wild-type HEK293T cell line (ab255449). Please note a wild-type cell line is not automatically included with a knockout cell line order, if required please add recommended wild-type cell line at no additional cost using the code WILDTYPE-TMTK1.
We will provide viable cells that proliferate on revival.
This product is subject to limited use licenses from The Broad Institute and ERS Genomics Limited, and is developed with patented technology. For full details of the limited use licenses and relevant patents please refer to our limited use license and patent pages.
What's included?
Properties and storage information
Gene name
Gene editing type
Gene editing method
Knockout validation
Shipped at conditions
Appropriate short-term storage conditions
Appropriate long-term storage conditions
Handling procedures
Initial handling guidelines
Upon arrival, the vial should be stored in liquid nitrogen vapor phase and not at -80°C. Storage at -80°C may result in loss of viability.
1. Thaw the vial in 37°C water bath for approximately 1-2 minutes.
2. Transfer the cell suspension (0.8 mL) to a 15 mL/50 mL conical sterile polypropylene centrifuge tube containing 8.4 mL pre-warmed culture medium, wash vial with an additional 0.8 mL culture medium (total volume 10 mL) to collect remaining cells, and centrifuge at 201 x g (rcf) for 5 minutes at room temperature. 10 mL represents minimum recommended dilution. 20 mL represents maximum recommended dilution.
3. Resuspend the cell pellet in 5 mL pre-warmed culture medium and count using a haemocytometer or alternative cell counting method seed all remaining cells into a T25.
4. Incubate the culture at 37°C incubator with 5% CO2. Check the culture one day after revival and continue to check until 80% confluent. Media change can be given if needed.
5. Once confluent passage into an appropriate flask at a density of 2x104 cells/cm2. Seeding density is given as a guide only and should be scaled to align with individual lab schedules. Cultures should be monitored daily.
Subculture guidelines
- All seeding densities should be based on cell counts gained by established methods.
- A guide seeding density of 2x104 cells/cm2 is recommended.
- Cells should be passaged when they have achieved 80-90% confluence.
Culture medium
DMEM (High Glucose) + 10% FBS
Cryopreservation medium
Cell Freezing Medium-DMSO Serum free media, contains 8.7% DMSO in MEM supplemented with methyl cellulose.
Supplementary information
This supplementary information is collated from multiple sources and compiled automatically.
Biological function summary
WDR34 plays a significant role in the regulation of ciliary assembly and maintenance. It forms a component of the intraflagellar transport (IFT) complex which is important for the functioning of primary cilia. Cilia are hair-like structures on cell surfaces that are important for signal transduction and environmental sensing. WDR34 contributes to the anterograde transport within cilia a process necessary for the proper assembly and function of the cilia structures. This involvement highlights its importance in maintaining cellular homeostasis through ciliary functions.
Pathways
The activity of WDR34 integrates into the Hedgehog signaling pathway which is critical in cell differentiation tissue patterning and embryonic development. It interacts with other IFT proteins such as IFT88 to modulate signal transduction processes within cilia. In the context of its role in ciliary transport WDR34 influences the activation and distribution of signaling molecules necessary for pathway functionality. The Hedgehog signaling is closely linked with various developmental processes indicating WDR34's importance in proper physiological development.
Quality control
STR analysis
D7S820, D5S818, TH01, D16S539, TPOX, CSF1PO, D13S317
Cell culture
Biosafety level
EU: 2 US: 2
Adherent/suspension
Adherent
Gender
Female
Target data
Complementary Products
Product promise
Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.
For licensing inquiries, please contact partnerships@abcam.com