WDR41 KO cell line available to order. Free of charge wild type control provided. Knockout achieved by using CRISPR/Cas9, 1 bp insertion in exon 1 and 4 bp deletion in exon 1 and 5 bp deletion in exon 1.
Images
Key facts
- Cell type
- HeLa
- Species or organism
- Human
- Tissue
- Cervix
- Form
- Liquid
- Knockout validation
- Sanger Sequencing
- Mutation description
- Knockout achieved by using CRISPR/Cas9, 1 bp insertion in exon 1 and 4 bp deletion in exon 1 and 5 bp deletion in exon 1
Alternative names
FLJ10904, MSTP048, WD repeat domain 41, WD repeat protein 41, WD repeat-containing protein 41, WDR41_HUMAN
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WDR41 KO cell line available to order. Free of charge wild type control provided. Knockout achieved by using CRISPR/Cas9, 1 bp insertion in exon 1 and 4 bp deletion in exon 1 and 5 bp deletion in exon 1.
Key facts
- Cell type
- HeLa
- Species or organism
- Human
- Tissue
- Cervix
- Form
- Liquid
- Knockout validation
- Sanger Sequencing
- Mutation description
- Knockout achieved by using CRISPR/Cas9, 1 bp insertion in exon 1 and 4 bp deletion in exon 1 and 5 bp deletion in exon 1
- Disease
- Adenocarcinoma
- Concentration
Properties
- Gene name
- WDR41
- Gene editing type
- Knockout
- Gene editing method
- CRISPR technology
- Knockout validation
- Sanger Sequencing
Quality control
- STR analysis
- CSF1PO, D13S317, D7S820, D5S818, TH01, D16S539, TPOX
Cell culture
- Biosafety level
- EU: 2 US: 2
- Adherent/suspension
- Adherent
- Gender
- Female
Handling procedures
- Initial handling guidelines
Upon arrival, the vial should be stored in liquid nitrogen vapor phase and not at -80°C. Storage at -80°C may result in loss of viability.
1. Thaw the vial in 37°C water bath for approximately 1-2 minutes.
2. Transfer the cell suspension (0.8 mL) to a 15 mL/50 mL conical sterile polypropylene centrifuge tube containing 8.4 mL pre-warmed culture medium, wash vial with an additional 0.8 mL culture medium (total volume 10 mL) to collect remaining cells, and centrifuge at 201 x g (rcf) for 5 minutes at room temperature. 10 mL represents minimum recommended dilution. 20 mL represents maximum recommended dilution.
3. Resuspend the cell pellet in 5 mL pre-warmed culture medium and count using a haemocytometer or alternative cell counting method seed all remaining cells into a T25.
4. Incubate the culture at 37°C incubator with 5% CO2. Check the culture one day after revival and continue to check until 80% confluent. Media change can be given if needed.
5. Once confluent passage into an appropriate flask at a density of 2x104 cells/cm2. Seeding density is given as a guide only and should be scaled to align with individual lab schedules. Cultures should be monitored daily.- Subculture guidelines
- All seeding densities should be based on cell counts gained by established methods.
- A guide seeding density of 2x104 cells/cm2 is recommended.
- Cells should be passaged when they have achieved 80-90% confluence.
- Culture medium
- DMEM (High Glucose) + 10% FBS
- Cryopreservation medium
- Cell Freezing Medium-DMSO Serum free media, contains 8.7% DMSO in MEM supplemented with methyl cellulose.
Storage
- Shipped at conditions
- Dry Ice
- Appropriate short-term storage conditions
- -196°C
- Appropriate long-term storage conditions
- -196°C
Notes
We will provide viable cells that proliferate on revival.
This product is subject to limited use licenses from The Broad Institute, ERS Genomics Limited and Sigma-Aldrich Co. LLC, and is developed with patented technology. For full details of the licenses and patents please refer to our limited use license and patent pages.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
WDR41 also known as WD repeat domain 41 is a protein that participates in cellular processes through its distinctive structural motif. The protein has multiple WD40 repeats and shows a molecular mass of approximately 74 kDa. WDR41 is found within the cytoplasm and also shows expression in various tissues across the human body including the brain and kidney. This wide expression implies its involvement in essential cellular activities.
- Biological function summary
WDR41 contributes to lysosomal function and acts as a component of the PI3P-binding WIPI3-WDR41 complex. The complex plays an important role in autophagy by promoting the formation of autophagosomes which are important for degrading and recycling cellular components. Additionally WDR41 participates in regulation of cellular homeostasis playing a role in maintaining the balance of proteins and organelles within the cell environment.
- Pathways
WDR41 plays a significant role in the autophagy and mTOR signaling pathways. These pathways are key in regulating cell growth proliferation and survival. Autophagy involves several proteins and complexes with mTORC1 being a vital regulator that signals the inhibition or promotion of this catabolic process. WDR41 interacts with other proteins such as LC3 and FYCO1 which help to mediate the autophagic response within cells and provide insights into its integration in these critical pathways.
- Associated diseases and disorders
Improper functioning of WDR41 links to neurodegenerative diseases and metabolic syndromes. Disruption in its expression may contribute to the development of conditions such as Parkinson’s disease where misregulation of autophagy leads to the accumulation of damaged organelles and proteins. Additionally WDR41 connections with the mTOR pathway can influence type 2 diabetes progression. Its interactions with proteins like ULK1 and Atg13 emphasize its role in these disease contexts highlighting its potential as a target for therapeutic interventions.
Product promise
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3 product images
Sanger Sequencing - Human WDR41 knockout HeLa cell line (ab265975)
Allele-1: 5 bp deletion in exon 1.
Sanger Sequencing - Human WDR41 knockout HeLa cell line (ab265975)
Allele-3: 1 bp insertion in exon 1.
Sanger Sequencing - Human WDR41 knockout HeLa cell line (ab265975)
Allele-2: 4 bp deletion in exon 1.
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