Human wild-type A549 cell line is not available for individual purchase. This product can be added free of charge to a KO cell line order of the same background.
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- International journal of molecular sciences 25:2024IRF1 Mediates Growth Arrest and the Induction of a Secretory Phenotype in Alveolar Epithelial Cells in Response to Inflammatory Cytokines IFNγ/TNFα.Applications:Unspecified applicationReactive species:Unspecified reactive speciesGiulia Recchia Luciani,Amelia Barilli,Rossana Visigalli,Roberto Sala,Valeria Dall'Asta,Bianca Maria RotoliPubMed 38542436
- Biomedicines 11:2023Cytokine-Induced iNOS in A549 Alveolar Epithelial Cells: A Potential Role in COVID-19 Lung Pathology.Applications:Unspecified applicationReactive species:Unspecified reactive speciesAmelia Barilli,Giulia Recchia Luciani,Rossana Visigalli,Roberto Sala,Maurizio Soli,Valeria Dall'Asta,Bianca Maria RotoliPubMed 37893073
Key facts
- Cell type
- A549
- Species or organism
- Human
- Tissue
- Lung
- Form
- Liquid
Recommended products
Human wild-type A549 cell line is not available for individual purchase. This product can be added free of charge to a KO cell line order of the same background.
Key facts
- Cell type
- A549
- Species or organism
- Human
- Tissue
- Lung
- Form
- Liquid
- Disease
- Carcinoma
- Concentration
Cell culture
- Biosafety level
- EU: 1 US: 1
- Adherent/suspension
- Adherent
- Gender
- Male
Handling procedures
- Initial handling guidelines
Upon arrival, the vial should be stored in liquid nitrogen vapor phase and not at -80°C. Storage at -80°C may result in loss of viability.
1. Thaw the vial in 37°C water bath for approximately 1-2 minutes.
2. Transfer the cell suspension (0.8 mL) to a 15 mL/50 mL conical sterile polypropylene centrifuge tube containing 8.4 mL pre-warmed culture medium, wash vial with an additional 0.8 mL culture medium (total volume 10 mL) to collect remaining cells, and centrifuge at 201 x g (rcf) for 5 minutes at room temperature. 10 mL represents minimum recommended dilution. 20 mL represents maximum recommended dilution.
3. Resuspend the cell pellet in 5 mL pre-warmed culture medium and count using a haemocytometer or alternative cell counting method seed all remaining cells into a T25.
4. Incubate the culture at 37°C incubator with 5% CO2. Check the culture one day after revival and continue to check until 80% confluent. Media change can be given if needed.
5. Once confluent passage into an appropriate flask at a density of 2x104 cells/cm2. Seeding density is given as a guide only and should be scaled to align with individual lab schedules. Cultures should be monitored daily.- Subculture guidelines
- All seeding densities should be based on cell counts gained by established methods.
- A guide seeding density of 2x104 cells/cm2 is recommended.
- Cells should be passaged when they have achieved 80-90% confluence.
- Do not allow the cell density to exceed 7x104 cells/cm2.
- Culture medium
- F-12K + 10% FBS
- Cryopreservation medium
- Cell Freezing Medium-DMSO Serum free media, contains 8.7% DMSO in MEM supplemented with methyl cellulose.
Storage
- Shipped at conditions
- Dry Ice
- Appropriate short-term storage conditions
- -196°C
- Appropriate long-term storage conditions
- -196°C
Notes
We will provide viable cells that proliferate on revival.
Product promise
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
2 product images
Sandwich ELISA - Human wild-type A549 cell line (ab255450)
Interpolated concentrations of native TRIM21/SS-A in human control wild type A549 (human lung carcinoma cell) and TRIM21 (TRIM21/SS-A) knockout A549 cell based on 200 µg/mL extract loads. The concentrations of TRIM21/SS-A were measured in duplicate and interpolated from the TRIM21/SS-A standard curve and corrected for sample dilution. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean TRIM21/SS-A concentration was determined to be 7026.2 pg/mL in wild type A549 extract (Human wild-type A549 cell line ab255450) and undetectable in TRIM21 (TRIM21/SS-A) knockout A549 extract (Human TRIM21 (TRIM21/SS-A) knockout A549 cell line Human TRIM21 (SS-A) knockout A549 cell line ab267025).
Western blot - Human wild-type A549 cell line (ab255450)
False colour image of Western blot: Anti-PD-L1 antibody [CAL10] – Mouse IgG1 (Chimeric); Rabbit anti-Vinculin antibody (Anti-Vinculin antibody [EPR20407] ab219649) loading control staining at 1/1000 dilution, shown in red. In Western blot, Anti-PD-L1 antibody [CAL10] - Mouse IgG1 (Chimeric) ab279292 was shown to bind specifically to PD-L1. A band was observed at 45 kDa in wild-type A549 cell lysates with no signal observed at this size in Cd274 knockout cell line. To generate this image, wild-type and Cd274 knockout A549 cell lysates were analysed. First, samples were run on an SDS-PAGE gel then transferred onto a nitrocellulose membrane. Membranes were blocked in 3 % milk in TBS-0.1 % Tween® 20 (TBS-T) before incubation with primary antibodies overnight at 4 °C. Blots were washed four times in TBS-T, incubated with secondary antibodies for 1 h at room temperature, washed again four times then imaged. Secondary antibodies used were Goat anti-Mouse IgG H&L 800CW and Goat anti-Rabbit IgG H&L 680RD at 1/20000 dilution.
All lanes: Western blot - Anti-PD-L1 antibody [CAL10] - Mouse IgG1 (Chimeric) (Anti-PD-L1 antibody [CAL10] - Mouse IgG1 (Chimeric) ab279292) at 1/1000 dilution
Lanes 1 - 2: Western blot - Human wild-type A549 cell line (ab255450)
Lane 1: Wild-type A549 Control IFN-gamma (0 ng/mL, 48 h) at 20 µg
Lane 2: Wild-type A549 Treated IFN-gamma (100 ng/mL, 48 h) at 20 µg
Lanes 3 - 4: Western blot - Human CD274 (PD-L1) knockout A549 cell line (Human CD274 (PD-L1) knockout A549 cell line ab267055)
Lane 3: CD274 knockout A549 Control IFN-gamma (0 ng/mL, 48 h) at 20 µg
Lane 4: CD274 knockout A549 Treated IFN-gamma (100 ng/mL, 48 h) at 20 µg
SecondaryAll lanes: Goat anti-Mouse IgG H&L 800CW and Goat anti-Rabbit IgG H&L 680RD at 1/20000 dilution
Performed under reducing conditions.
Predicted band size: 33 kDa
Observed band size: 45 kDa
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