Human wild-type HEK-293T cell line is not available for individual purchase. This product can be added free of charge to a KO cell line order of the same background.
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- iScience 27:1101612024Cytokine-like-Vago-mediated antiviral response in via IKK-NF-κB signaling pathway.Applications:Unspecified applicationReactive species:Unspecified reactive speciesZittipong Nanakorn,Taro Kawai,Anchalee TassanakajonPubMed 38974974
- Nature cell biology 26:552-5662024Glucose controls lipolysis through Golgi PtdIns4P-mediated regulation of ATGL.Applications:Unspecified applicationReactive species:Unspecified reactive speciesLianggong Ding,Florian Huwyler,Fen Long,Wu Yang,Jonas Binz,Kendra Wernlé,Matthias Pfister,Manuel Klug,Miroslav Balaz,Barbara Ukropcova,Jozef Ukropec,Chunyan Wu,Tongtong Wang,Min Gao,Pierre-Alain Clavien,Philipp Dutkowski,Mark W Tibbitt,Christian WolfrumPubMed 38561547
- Nature communications 15:24042024Structure of full-length ERGIC-53 in complex with MCFD2 for cargo transport.Applications:Unspecified applicationReactive species:Unspecified reactive speciesSatoshi Watanabe,Yoshiaki Kise,Kento Yonezawa,Mariko Inoue,Nobutaka Shimizu,Osamu Nureki,Kenji InabaPubMed 38493152
- iScience 26:1057932023Prx4 acts as DAMP in shrimp, enhancing bacterial resistance via the toll pathway and prophenoloxidase activation.Applications:Unspecified applicationReactive species:Unspecified reactive speciesSupitcha Wanvimonsuk,Phattarunda Jaree,Taro Kawai,Kunlaya SomboonwiwatPubMed 36619979
- STAR protocols 4:1019052022Prediction and verification of glycosyltransferase activity by bioinformatics analysis and protein engineering.Applications:Unspecified applicationReactive species:Unspecified reactive speciesDietlind L Gerloff,Elena I Ilina,Camille Cialini,Uxue Mata Salcedo,Michel Mittelbronn,Tanja MüllerPubMed 36528856
- International journal of molecular sciences 23:2022Chitosan versus Carboxymethyl Chitosan Cryogels: Bacterial Colonization, Human Embryonic Kidney 293T Cell Culturing and Co-Culturing.Applications:Unspecified applicationReactive species:Unspecified reactive speciesAndrey Boroda,Yuliya Privar,Mariya Maiorova,Irina Beleneva,Marina Eliseikina,Anna Skatova,Dmitry Marinin,Svetlana BratskayaPubMed 36293131
- Nature metabolism 3:1648-16612021Peroxisomal β-oxidation acts as a sensor for intracellular fatty acids and regulates lipolysis.Applications:Unspecified applicationReactive species:Unspecified reactive speciesLianggong Ding,Wenfei Sun,Miroslav Balaz,Anyuan He,Manuel Klug,Stefan Wieland,Robert Caiazzo,Violeta Raverdy,Francois Pattou,Philippe Lefebvre,Irfan J Lodhi,Bart Staels,Markus Heim,Christian WolfrumPubMed 34903883
- Journal of virology 95:e00807212021-(4-Hydroxyphenyl) Retinamide Suppresses SARS-CoV-2 Spike Protein-Mediated Cell-Cell Fusion by a Dihydroceramide Δ4-Desaturase 1-Independent Mechanism.Applications:Unspecified applicationReactive species:Unspecified reactive speciesYasuhiro Hayashi,Kiyoto Tsuchiya,Mizuki Yamamoto,Yoko Nemoto-Sasaki,Kazunari Tanigawa,Kotaro Hama,Yusuke Ueda,Takashi Tanikawa,Jin Gohda,Kenji Maeda,Jun-Ichiro Inoue,Atsushi YamashitaPubMed 34106748
Key facts
- Cell type
HEK-293
- Species or organism
Human
- Tissue
Kidney
- Form
Liquid
Recommended products
Human wild-type HEK-293T cell line is not available for individual purchase. This product can be added free of charge to a KO cell line order of the same background.
Key facts
- Cell type
HEK-293
- Species or organism
Human
- Tissue
Kidney
- Form
Liquid
- Concentration
Cell culture
- Biosafety level
EU: 2 US: 2
- Adherent/suspension
Adherent
- Gender
Female
Handling procedures
- Initial handling guidelines
Upon arrival, the vial should be stored in liquid nitrogen vapor phase and not at -80°C. Storage at -80°C may result in loss of viability.
1. Thaw the vial in 37°C water bath for approximately 1-2 minutes.
2. Transfer the cell suspension (0.8 mL) to a 15 mL/50 mL conical sterile polypropylene centrifuge tube containing 8.4 mL pre-warmed culture medium, wash vial with an additional 0.8 mL culture medium (total volume 10 mL) to collect remaining cells, and centrifuge at 201 x g (rcf) for 5 minutes at room temperature. 10 mL represents minimum recommended dilution. 20 mL represents maximum recommended dilution.
3. Resuspend the cell pellet in 5 mL pre-warmed culture medium and count using a haemocytometer or alternative cell counting method seed all remaining cells into a T25.
4. Incubate the culture at 37°C incubator with 5% CO2. Check the culture one day after revival and continue to check until 80% confluent. Media change can be given if needed.
5. Once confluent passage into an appropriate flask at a density of 2x104 cells/cm2. Seeding density is given as a guide only and should be scaled to align with individual lab schedules. Cultures should be monitored daily.- Subculture guidelines
- All seeding densities should be based on cell counts gained by established methods.
- A guide seeding density of 2x104 cells/cm2 is recommended.
- Cells should be passaged when they have achieved 80-90% confluence.
- Culture medium
DMEM (High Glucose) + 10% FBS
- Cryopreservation medium
Cell Freezing Medium-DMSO Serum free media, contains 8.7% DMSO in MEM supplemented with methyl cellulose.
Storage
- Shipped at conditions
Dry Ice
- Appropriate long-term storage conditions
-196°C
Notes
Wild-type cell lines are sold with knockout cell lines only - not available for individual purchase.
We will provide viable cells that proliferate on revival.
Product promise
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
4 product images
Western blot - Human wild-type HEK-293T cell line (ab255449)
This data was developed using Anti-GAK antibody [EPR25327-102] ab288566, the same antibody clone in a different buffer formulation.
Blocking and diluting buffer and concentration: 5% NFDM/TBST
All lanes: Western blot - Anti-GAK antibody [EPR25327-102] (Anti-GAK antibody [EPR25327-102] ab288566) at 1/1000 dilution
Lane 1: Western blot - Human wild-type HEK-293T cell line (ab255449) at 20 µg
Lane 2: Western blot - Human GAK knockout HEK-293T cell line (Human GAK knockout HEK-293T cell line ab266417) at 20 µg
Lane 3: Daudi (Human Burkitt’s lymphoma lymphoblast), whole cell lysate at 20 µg
SecondaryAll lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/100000 dilution
Predicted band size: 143 kDa
Observed band size: 160 kDa
Exposure time: 59s
Sandwich ELISA - Human wild-type HEK-293T cell line (ab255449)
Sandwich ELISA - Human wild-type HEK-293T cell line (ab255449)
Interpolated concentrations of native c-Myc in human control wild type HEK293T cell and MYC knockout HEK293T cell based on 1000 µg/mL extract loads. The concentrations of c-Myc were measured in duplicate and interpolated from the c-Myc standard curve and corrected for sample dilution. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean c-Myc concentration was determined to be 4509.9 pg/mL in wild type HEK293T extract (Human wild-type HEK293T cell line ab255449) and undetectable in MYC knockout HEK293T extract (Human MYC knockout HEK293T cell line Human MYC (c-Myc) knockout HEK-293T cell line ab256500).
Western blot - Human wild-type HEK-293T cell line (ab255449)
All lanes: Western blot - Anti-PAK4 antibody [EPR28630-82] (Anti-PAK4 antibody [EPR28630-82] ab314765) at 1/1000 dilution
Lane 1: Western blot - Human wild-type HEK-293T cell line (ab255449) at 20 µg
Lane 2: Western blot - Human PAK4 knockout HEK-293T cell lysate (Human PAK4 knockout HEK-293T cell lysate ab258560) at 20 µg
Lane 3: PANC-1 (human pancreatic epithelioid carcinoma epithelial cell) whole cell lysate at 20 µg
Lane 4: Human testis lysate at 20 µg
SecondaryAll lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/20000 dilution
Performed under reducing conditions.
Observed band size: 52 kDa, 68 kDa
Exposure time: 70s
Blocking and diluting buffer and concentration: 5% NFDM/TBST.
In Western blot, Anti-PAK4 antibody [EPR28630-82] ab314765 was shown to bind specifically to PAK4. Target of interest was observed at 68 kDa and 52 KD in wild-type HEK293T cell lysates (lane 1, wild-type cell line ab255449) with no signal observed at this size in PAK4 knockout cell line (lane 2, knockout cell line Human PAK4 knockout HEK-293T cell line ab266807/ knockout cell lysate Human PAK4 knockout HEK-293T cell lysate ab258560). The band (50 kDa) beneath the target band (68 kDa) is likely to be degraded target fragments. In western blot, anti-GAPDH antibody [EPR16891] - Loading Control (Anti-GAPDH antibody [EPR16891] - Loading Control ab181602) staining at 1/200000 dilution.
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