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Human wild-type HeLa cell line is not available for individual purchase. This product can be added free of charge to a KO cell line order of the same background.


Images

Sandwich ELISA - Human wild-type HeLa cell line (AB255928), expandable thumbnail
  • Sandwich ELISA - Human wild-type HeLa cell line (AB255928), expandable thumbnail
  • Sandwich ELISA - Human wild-type HeLa cell line (AB255928), expandable thumbnail

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Key facts

Cell type

HeLa

Species or organism

Human

Tissue

Cervix

Form

Liquid

Recommended products

Human wild-type HeLa cell line is not available for individual purchase. This product can be added free of charge to a KO cell line order of the same background.

Key facts

Cell type

HeLa

Form

Liquid

Disease

Adenocarcinoma

Concentration
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Cell culture

Biosafety level

EU: 2 US: 2

Adherent/suspension

Adherent

Gender

Female

Handling procedures

Initial handling guidelines

Upon arrival, the vial should be stored in liquid nitrogen vapor phase and not at -80°C. Storage at -80°C may result in loss of viability.

1. Thaw the vial in 37°C water bath for approximately 1-2 minutes.
2. Transfer the cell suspension (0.8 mL) to a 15 mL/50 mL conical sterile polypropylene centrifuge tube containing 8.4 mL pre-warmed culture medium, wash vial with an additional 0.8 mL culture medium (total volume 10 mL) to collect remaining cells, and centrifuge at 201 x g (rcf) for 5 minutes at room temperature. 10 mL represents minimum recommended dilution. 20 mL represents maximum recommended dilution.
3. Resuspend the cell pellet in 5 mL pre-warmed culture medium and count using a haemocytometer or alternative cell counting method seed all remaining cells into a T25.
4. Incubate the culture at 37°C incubator with 5% CO2. Check the culture one day after revival and continue to check until 80% confluent. Media change can be given if needed.
5. Once confluent passage into an appropriate flask at a density of 2x104 cells/cm2. Seeding density is given as a guide only and should be scaled to align with individual lab schedules. Cultures should be monitored daily.

Subculture guidelines

  • All seeding densities should be based on cell counts gained by established methods.

  • A guide seeding density of 2x104 cells/cm2 is recommended.

  • Cells should be passaged when they have achieved 80-90% confluence.

Culture medium

DMEM (High Glucose) + 10% FBS

Cryopreservation medium

Cell Freezing Medium-DMSO Serum free media, contains 8.7% DMSO in MEM supplemented with methyl cellulose.

Storage

Shipped at conditions

Dry Ice

Appropriate short-term storage conditions

-196°C

Appropriate long-term storage conditions

-196°C

Notes

Wild-type cell lines are sold with knockout cell lines only - not available for individual purchase.

We will provide viable cells that proliferate on revival.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

3 product images

  • Sandwich ELISA - Human wild-type HeLa cell line (ab255928), expandable thumbnail

    Sandwich ELISA - Human wild-type HeLa cell line (ab255928)

    Human CDC25C concentration was interpolated from the CDC25C standard curve. Lysates from cell culture samples were serially diluted and assessed by the Human Cdc25C ELISA Kit (SimpleStep) (Human Cdc25C ELISA Kit ab230944). Wild-type HeLa (ab255928), CDC25C knockout HeLa (Human CDC25C knockout HeLa cell line ab265189) and Jurkat cells were assessed in duplicate (n=2). Data are represented as the mean and error bars represent standard deviation.

  • Sandwich ELISA - Human wild-type HeLa cell line (ab255928), expandable thumbnail

    Sandwich ELISA - Human wild-type HeLa cell line (ab255928)

    Sandwich ELISA of Human Cathepsin C Antibody Pair - BSA and Azide free ab314793 with the capture antibody dilution at 2 µg/mL and detector antibody dilution at 0.5 µg/mL. Interpolated concentrations of native Cathepsin C in human control wild type HeLa cell and CTSC (Cathepsin C) knockout HeLa cell based on 2.5 µg/mL extract loads. The concentrations of Cathepsin C were measured in duplicate and interpolated from the Cathepsin C standard curve and corrected for sample dilution. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean Cathepsin C concentration was determined to be 310.1 pg/mL in wild type HeLa extract (Human wild-type HeLa cell line ab255928) and undetectable in CTSC (Cathepsin C) knockout HeLa extract (Human CTSC (Cathepsin C) knockout HeLa cell line Human CTSC (Cathepsin C) knockout HeLa cell line ab265822).

  • Sandwich ELISA - Human wild-type HeLa cell line (ab255928), expandable thumbnail

    Sandwich ELISA - Human wild-type HeLa cell line (ab255928)

    Sandwich ELISA of Human PTRF Antibody Pair - BSA and Azide free ab312881 with the capture antibody dilution at 2 µg/mL and detector antibody dilution at 0.5 µg/mL.

    Interpolated concentrations of native PTRF in human control wild type HeLa cell and PTRF knockout HeLa cell based on 25 µg/mL extract loads. The concentrations of PTRF were measured in duplicate and interpolated from the PTRF standard curve and corrected for sample dilution. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean PTRF concentration was determined to be 281.5 pg/mL in wild type HeLa extract (Human wild-type HeLa cell line ab255928) and undetectable in PTRF knockout HeLa extract (Human PTRF knockout HeLa cell line Human PTRF knockout HeLa cell line ab265435).

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com