Human XIAP knockout A549 cell line
- Advanced Validation
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XIAP KO cell line available to order. KO validated by Western blot. Free of charge wild type control provided.
View Alternative Names
AP 13, API3, Apoptosis Inhibitor 3, BIRC 4, Baculoviral IAP repeat containing 4, Baculoviral IAP repeat-containing protein 4, E3 ubiquitin-protein ligase XIAP, HILP, IAP-3, IAP-like protein, ILP, ILP 1, Inhibitor of Apoptosis X Linked, Inhibitor of apoptosis protein 3, MIHA, Mammalian IAP Homologue A, X linked inhibitor of apoptosis, X linked inhibitor of apoptosis E3 ubiquitin protein ligase, X-linked IAP, X-linked inhibitor of apoptosis protein, XIAP_HUMAN, XLP2, hIAP-3, x-linked inhibitor of apopotosis proteins
- WB
Lab
Western blot - Human XIAP knockout A549 cell line (AB289043)
False colour image of Western blot : Anti-XIAP antibody [EPR22189-113] staining at 1/1000 dilution, shown in green; Mouse anti-GAPDH antibody [6C5] (ab8245) loading control staining at 1/20000 dilution, shown in red. In Western blot, ab229050 was shown to bind specifically to XIAP. A band was observed at 60 kDa in wild-type A549 cell lysates with no signal observed at this size in XIAP knockout cell line ab289043. To generate this image, wild-type and XIAP knockout A549 cell lysates were analysed. First, samples were run on an SDS-PAGE gel then transferred onto a nitrocellulose membrane. Membranes were blocked in 3 % milk in TBS-0.1 % Tween® 20 (TBS-T) before incubation with primary antibodies overnight at 4 °C. Blots were washed four times in TBS-T, incubated with secondary antibodies for 1 h at room temperature, washed again four times then imaged. Secondary antibodies used were Goat anti-Rabbit IgG H&L 800CW and Goat anti-Mouse IgG H&L 680RD at 1/20000 dilution.
All lanes:
Western blot - Anti-XIAP antibody [EPR22189-113] (<a href='/en-us/products/primary-antibodies/xiap-antibody-epr22189-113-ab229050'>ab229050</a>) at 1/1000 dilution
Lane 1:
Wild-type A549 cell lysate at 20 µg
Lane 2:
XIAP knockout A549 cell lysate at 20 µg
Lane 3:
MCF7 cell lysate at 20 µg
Lane 4:
MOLT-4 cell lysate at 20 µg
Observed band size: 60 kDa
false
Reactivity data
Product details
Although we aim to provide customers with a homozygous clone, feasibility will be dependent on the biology of the protein. Should only heterozygous edits be achieved, you will be notified of the outcome and be asked to confirm whether the cell line is acceptable. All clones will be accompanied with DNA sequencing data, and the mutation description.
Recommended control: Human wild-type A549 cell line (ab288558). Please note a wild-type cell line is not automatically included with a knockout cell line order, if required please add recommended wild-type cell line at no additional cost using the code WILDTYPE-TMTK1.
We will provide viable cells that proliferate on revival.
This product is subject to limited use licenses from The Broad Institute and ERS Genomics Limited, and is developed with patented technology. For full details of the limited use licenses and relevant patents please refer to our limited use license and patent pages.
Properties and storage information
Gene name
Gene editing type
Gene editing method
Knockout validation
Shipped at conditions
Appropriate short-term storage conditions
Appropriate long-term storage conditions
Handling procedures
Initial handling guidelines
Upon arrival, the vial should be stored in liquid nitrogen vapor phase and not at -80°C. Storage at -80°C may result in loss of viability.
1. Thaw the vial in 37°C water bath for approximately 1-2 minutes.
2. Transfer the cell suspension (0.8 mL) to a 15 mL/50 mL conical sterile polypropylene centrifuge tube containing 8.4 mL pre-warmed culture medium, wash vial with an additional 0.8 mL culture medium (total volume 10 mL) to collect remaining cells, and centrifuge at 201 x g (rcf) for 5 minutes at room temperature. 10 mL represents minimum recommended dilution. 20 mL represents maximum recommended dilution.
3. Resuspend the cell pellet in 5 mL pre-warmed culture medium and count using a haemocytometer or alternative cell counting method seed all remaining cells into a T25.
4. Incubate the culture at 37°C incubator with 5% CO2. Check the culture one day after revival and continue to check until 80% confluent. Media change can be given if needed.
5. Once confluent passage into an appropriate flask at a density of 2x104 cells/cm2. Seeding density is given as a guide only and should be scaled to align with individual lab schedules. Cultures should be monitored daily.
Subculture guidelines
- All seeding densities should be based on cell counts gained by established methods.
- A guide seeding density of 2x104 cells/cm2 is recommended.
- Cells should be passaged when they have achieved 80-90% confluence.
- Do not allow the cell density to exceed 7x104 cells/cm2.
Culture medium
F-12K + 10% FBS
Cryopreservation medium
Cell Freezing Medium-DMSO Serum free media, contains 8.7% DMSO in MEM supplemented with methyl cellulose.
Supplementary information
This supplementary information is collated from multiple sources and compiled automatically.
Biological function summary
The function of XIAP involves regulation of apoptosis an important process in maintaining cellular homeostasis. It forms part of the apoptosome complex by interacting with caspases to prevent premature cell death. XIAP serves to protect cells from stress-induced apoptotic signals helping in survival under adverse conditions. This ability makes it a subject of interest in studying cancer survival mechanisms since overactive XIAP can allow cancer cells to avoid programmed cell death.
Pathways
Apoptosis regulation and cell survival pathways prominently feature XIAP. Particularly XIAP plays a role in the intrinsic apoptotic pathway which is activated in response to internal stress signals. XIAP regulates this pathway via its interaction with caspase-9 and interactions with other proteins such as Smac/DIABLO which can antagonize XIAP's function. This interaction is key in balancing cell death and survival therefore highlighting its role in apoptosis control.
Quality control
STR analysis
CSF1PO, D13S317, D7S820, D5S818, TH01, D16S539, TPOX
Cell culture
Biosafety level
EU: 1 US: 1
Adherent/suspension
Adherent
Gender
Male
Target data
Complementary Products
![Western blot - Anti-XIAP antibody [EPR22189-113] (AB229050)](https://content.abcam.com/products/images/xiap-antibody-epr22189-113-ab229050--western-blot-img13934.jpg)
Product promise
Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.
For licensing inquiries, please contact partnerships@abcam.com