YIPF3 KO cell line available to order. Free of charge wild type control provided. Knockout achieved by using CRISPR/Cas9, Homozygous: 50 bp deletion in exon 1.
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Key facts
- Cell type
- HEK-293T
- Species or organism
- Human
- Tissue
- Kidney
- Form
- Liquid
- Knockout validation
- Sanger Sequencing
- Mutation description
- Knockout achieved by using CRISPR/Cas9, Homozygous: 50 bp deletion in exon 1
Alternative names
C6orf109, FinGER3, KLIP1, Killer lineage protein 1, Natural killer cell-specific antigen KLIP1, Protein YIPF3, YIP1 family member 3, YIPF3_HUMAN, Yip1 domain family member 3, dJ337H4.3
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YIPF3 KO cell line available to order. Free of charge wild type control provided. Knockout achieved by using CRISPR/Cas9, Homozygous: 50 bp deletion in exon 1.
Key facts
- Cell type
- HEK-293T
- Species or organism
- Human
- Tissue
- Kidney
- Form
- Liquid
- Knockout validation
- Sanger Sequencing
- Mutation description
- Knockout achieved by using CRISPR/Cas9, Homozygous: 50 bp deletion in exon 1
- Concentration
Properties
- Gene name
- YIPF3
- Gene editing type
- Knockout
- Gene editing method
- CRISPR technology
- Knockout validation
- Sanger Sequencing
- Zygosity
- Homozygous
Quality control
- STR analysis
- CSF1PO, D13S317, D7S820, D5S818, TH01, D16S539, TPOX
Cell culture
- Biosafety level
- EU: 2 US: 2
- Adherent/suspension
- Adherent
- Gender
- Female
Handling procedures
- Initial handling guidelines
Upon arrival, the vial should be stored in liquid nitrogen vapor phase and not at -80°C. Storage at -80°C may result in loss of viability.
1. Thaw the vial in 37°C water bath for approximately 1-2 minutes.
2. Transfer the cell suspension (0.8 mL) to a 15 mL/50 mL conical sterile polypropylene centrifuge tube containing 8.4 mL pre-warmed culture medium, wash vial with an additional 0.8 mL culture medium (total volume 10 mL) to collect remaining cells, and centrifuge at 201 x g (rcf) for 5 minutes at room temperature. 10 mL represents minimum recommended dilution. 20 mL represents maximum recommended dilution.
3. Resuspend the cell pellet in 5 mL pre-warmed culture medium and count using a haemocytometer or alternative cell counting method seed all remaining cells into a T25.
4. Incubate the culture at 37°C incubator with 5% CO2. Check the culture one day after revival and continue to check until 80% confluent. Media change can be given if needed.
5. Once confluent passage into an appropriate flask at a density of 2x104 cells/cm2. Seeding density is given as a guide only and should be scaled to align with individual lab schedules. Cultures should be monitored daily.- Subculture guidelines
- All seeding densities should be based on cell counts gained by established methods.
- A guide seeding density of 2x104 cells/cm2 is recommended.
- Cells should be passaged when they have achieved 80-90% confluence.
- Culture medium
- DMEM (High Glucose) + 10% FBS
- Cryopreservation medium
- Cell Freezing Medium-DMSO Serum free media, contains 8.7% DMSO in MEM supplemented with methyl cellulose.
Storage
- Shipped at conditions
- Dry Ice
- Appropriate short-term storage conditions
- -196°C
- Appropriate long-term storage conditions
- -196°C
Notes
We will provide viable cells that proliferate on revival.
This product is subject to limited use licenses from The Broad Institute and ERS Genomics Limited, and is developed with patented technology. For full details of the limited use licenses and relevant patents please refer to our limited use license and patent pages.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
YIPF3 also known as YIP1 family member 3 is a protein with a molecular mass of approximately 41 kDa. It localizes mainly to the Golgi apparatus and endoplasmic reticulum (ER) within cells. YIPF3 is highly expressed in a variety of tissues including liver kidney and brain indicating its widespread function throughout the body. This protein belongs to the Yip1 family which is associated with membrane trafficking processes.
- Biological function summary
The main role of YIPF3 involves regulation of the secretory pathway. It functions as part of a larger protein complex interacting with other members of the YIP family as well as Rab GTPases. YIPF3 contributes to the maintenance and formation of Golgi morphology ensuring proper vesicle trafficking between the ER and Golgi compartments. This activity is essential for efficient processing and transport of proteins and lipids in the cell.
- Pathways
The involvement of YIPF3 can prominently be seen in the secretory and transport pathways of eukaryotic cells. It associates with the Rab GTPase pathway influencing vesicle budding and fusion essential for cellular transport mechanisms. YIPF3 works alongside Rab proteins to facilitate correct vesicular movement and signal transduction ensuring proper protein sorting and secretion.
- Associated diseases and disorders
Disruptions involving YIPF3 have connections to neurodegenerative diseases and certain cancers. Dysfunction in YIPF3 activity can lead to impaired vesicle trafficking contributing to neuron degeneration seen in neuronal disorders. Additionally alterations in YIPF3 expression levels have been observed in some oncological diseases highlighting its connection to cancer pathways. Its interplay with proteins such as Rab GTPases further illustrates the potential for broader impacts on disease development and progression.
Product promise
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1 product image
Sanger Sequencing - Human YIPF3 knockout HEK-293T cell line (ab266746)
Homozygous: 50 bp deletion in exon 1
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