ZCCHC2 KO cell line available to order. Free of charge wild type control provided. Knockout achieved by using CRISPR/Cas9, Homozygous: 1 bp insertion in exon 1.
Images
Key facts
- Cell type
- A549
- Species or organism
- Human
- Tissue
- Lung
- Form
- Liquid
- Knockout validation
- Sanger Sequencing
- Mutation description
- Knockout achieved by using CRISPR/Cas9, Homozygous: 1 bp insertion in exon 1
Alternative names
9930114B20Rik, AW212015, DKFZp451A185, FLJ20281, KIAA1744, MGC112793, MGC13269, TNFRSF11A, ZCHC2_HUMAN, Zinc finger CCHC domain-containing protein 2
Recommended products
ZCCHC2 KO cell line available to order. Free of charge wild type control provided. Knockout achieved by using CRISPR/Cas9, Homozygous: 1 bp insertion in exon 1.
Key facts
- Cell type
- A549
- Species or organism
- Human
- Tissue
- Lung
- Form
- Liquid
- Knockout validation
- Sanger Sequencing
- Mutation description
- Knockout achieved by using CRISPR/Cas9, Homozygous: 1 bp insertion in exon 1
- Disease
- Carcinoma
- Concentration
Properties
- Gene name
- ZCCHC2
- Gene editing type
- Knockout
- Gene editing method
- CRISPR technology
- Knockout validation
- Sanger Sequencing
- Zygosity
- Homozygous
Quality control
- STR analysis
- CSF1PO, D13S317, D7S820, D5S818, TH01, D16S539, TPOX
Cell culture
- Biosafety level
- EU: 1 US: 1
- Adherent/suspension
- Adherent
- Gender
- Male
Handling procedures
- Initial handling guidelines
Upon arrival, the vial should be stored in liquid nitrogen vapor phase and not at -80°C. Storage at -80°C may result in loss of viability.
1. Thaw the vial in 37°C water bath for approximately 1-2 minutes.
2. Transfer the cell suspension (0.8 mL) to a 15 mL/50 mL conical sterile polypropylene centrifuge tube containing 8.4 mL pre-warmed culture medium, wash vial with an additional 0.8 mL culture medium (total volume 10 mL) to collect remaining cells, and centrifuge at 201 x g (rcf) for 5 minutes at room temperature. 10 mL represents minimum recommended dilution. 20 mL represents maximum recommended dilution.
3. Resuspend the cell pellet in 5 mL pre-warmed culture medium and count using a haemocytometer or alternative cell counting method seed all remaining cells into a T25.
4. Incubate the culture at 37°C incubator with 5% CO2. Check the culture one day after revival and continue to check until 80% confluent. Media change can be given if needed.
5. Once confluent passage into an appropriate flask at a density of 2x104 cells/cm2. Seeding density is given as a guide only and should be scaled to align with individual lab schedules. Cultures should be monitored daily.- Subculture guidelines
- All seeding densities should be based on cell counts gained by established methods.
- A guide seeding density of 2x104 cells/cm2 is recommended.
- Cells should be passaged when they have achieved 80-90% confluence.
- Do not allow the cell density to exceed 7x104 cells/cm2.
- Culture medium
- F-12K + 10% FBS
- Cryopreservation medium
- Cell Freezing Medium-DMSO Serum free media, contains 8.7% DMSO in MEM supplemented with methyl cellulose.
Storage
- Shipped at conditions
- Dry Ice
- Appropriate short-term storage conditions
- -196°C
- Appropriate long-term storage conditions
- -196°C
Notes
We will provide viable cells that proliferate on revival.
This product is subject to limited use licenses from The Broad Institute, ERS Genomics Limited and Sigma-Aldrich Co. LLC, and is developed with patented technology. For full details of the licenses and patents please refer to our limited use license and patent pages.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
The zinc finger CCHC domain-containing protein 2 also known as ZCCHC2 functions mechanically as an RNA-binding protein. It plays a role in RNA metabolic processes. ZCCHC2 is approximately 90 kDa in mass and includes several zinc finger motifs that are important for its interaction with RNA. It is expressed highly in the nucleus of cells across various tissues with notable levels in the brain and other central nervous system structures. This indicates its involvement in regulating RNA under specific physiological conditions.
- Biological function summary
ZCCHC2 interacts with the cellular machinery involved in RNA splicing and processing. It is part of ribonucleoprotein complexes that facilitate these functions. This protein assists in modulating RNA stability and contributes to the precise processing required for mature RNA molecules. These activities are important for maintaining proper gene expression by ensuring that RNA transcripts are accurately produced and modified before translation occurs.
- Pathways
ZCCHC2 plays a role in RNA processing and degradation pathways. It acts within the spliceosomal complex aiding in the proper removal of introns from pre-mRNA. ZCCHC2 interacts with other proteins such as components of the Human spliceosome which include splicing factors that orchestrate the splicing process. It also connects with pathways linked to RNA surveillance mechanisms where it helps in quality control of RNA transcripts.
- Associated diseases and disorders
ZCCHC2 has connections to neurological conditions and cancer. Abnormal expression levels or mutations in ZCCHC2 may relate to altered splicing activities leading to neurodevelopmental disorders. Additionally its dysregulation might contribute to the genesis of certain cancers through disrupted RNA processing. Proteins like spliceosomal components and RNA-binding proteins are usually involved in such diseases highlighting the importance of balanced ZCCHC2 function for cellular health.
Product promise
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
1 product image
Sanger Sequencing - Human ZCCHC2 knockout A549 cell line (ab267174)
Homozygous: 1 bp insertion in exon1
Downloads
Product protocols
- Visit the general protocols
- Visit troubleshooting
Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.
For licensing inquiries, please contact partnerships@abcam.com