ZNF593 KO cell line available to order. Free of charge wild type control provided. Knockout achieved by using CRISPR/Cas9, Homozygous: 17 bp deletion in exon 1.
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Key facts
- Cell type
- HEK-293T
- Species or organism
- Human
- Tissue
- Kidney
- Form
- Liquid
- Knockout validation
- Sanger Sequencing
- Mutation description
- Knockout achieved by using CRISPR/Cas9, Homozygous: 17 bp deletion in exon 1
Alternative names
ZN593_HUMAN, ZT86, Zinc finger protein 593, Zinc finger protein LOC51042, Zinc finger protein T86
Recommended products
ZNF593 KO cell line available to order. Free of charge wild type control provided. Knockout achieved by using CRISPR/Cas9, Homozygous: 17 bp deletion in exon 1.
Key facts
- Cell type
- HEK-293T
- Species or organism
- Human
- Tissue
- Kidney
- Form
- Liquid
- Knockout validation
- Sanger Sequencing
- Mutation description
- Knockout achieved by using CRISPR/Cas9, Homozygous: 17 bp deletion in exon 1
- Concentration
Properties
- Gene name
- ZNF593
- Gene editing type
- Knockout
- Gene editing method
- CRISPR technology
- Knockout validation
- Sanger Sequencing
- Zygosity
- Homozygous
Quality control
- STR analysis
- CSF1PO, D13S317, D7S820, D5S818, TH01, D16S539, TPOX
Cell culture
- Biosafety level
- EU: 2 US: 2
- Adherent/suspension
- Adherent
- Gender
- Female
Handling procedures
- Subculture guidelines
- All seeding densities should be based on cell counts gained by established methods.
- A guide seeding density of 2x104 cells/cm2 is recommended.
- Cells should be passaged when they have achieved 80-90% confluence.
- Culture medium
- DMEM (High Glucose) + 10% FBS
- Cryopreservation medium
- Cell Freezing Medium-DMSO Serum free media, contains 8.7% DMSO in MEM supplemented with methyl cellulose.
Storage
- Shipped at conditions
- Dry Ice
- Appropriate short-term storage conditions
- -196°C
- Appropriate long-term storage conditions
- -196°C
Notes
We will provide viable cells that proliferate on revival.
This product is subject to limited use licenses from The Broad Institute and ERS Genomics Limited, and is developed with patented technology. For full details of the limited use licenses and relevant patents please refer to our limited use license and patent pages.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
ZNF593 also known as Zinc Finger Protein 593 is a protein with a mass of approximately 30 kDa. It functions as a transcriptional regulator modulating gene expression through binding DNA. ZNF593 is expressed mainly in the nucleus of cells with a concentration in tissues such as the liver kidney and heart. Its zinc finger motif suggests a role in DNA binding and regulatory functions.
- Biological function summary
ZNF593 is essential in managing the transcriptional activities within cells. It associates with a multiprotein complex interacting with other proteins to influence transcription. This protein plays a role in regulating cell proliferation and differentiation impacting how cells specialize and grow. Its involvement in interaction with RNA polymerase II highlights its role in gene expression regulation making it important in managing various cellular processes.
- Pathways
This protein enhances our understanding of cellular signaling and communication. ZNF593 is involved in the MAPK/ERK and JAK/STAT signaling pathways. These pathways control essential processes like cell growth differentiation and response to external stimuli. ZNF593 interacts directly with key proteins within these pathways such as ERK and STAT which further supports its role in modulating cell fate decisions and signaling cascades.
- Associated diseases and disorders
ZNF593’s deregulation links to certain cancers and developmental disorders. Abnormal expression of ZNF593 correlates with progression in liver cancer where it impacts cell proliferation and survival. In addition mutations affecting ZNF593 have been associated with intellectual disabilities. Its interaction with proteins like STAT in these contexts underlines its pathological significance emphasizing the importance of proper regulation for maintaining cellular health.
Product promise
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Terms & Conditions.
1 product image
Sanger Sequencing - Human ZNF593 knockout HEK-293T cell line (ab266205)
Homozygous: 17 bp deletion in exon 1
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Product protocols
- Visit the general protocols
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