ZNHIT1 KO cell line available to order. Free of charge wild type control provided. Knockout achieved by using CRISPR/Cas9, Homozygous: 215 bp insertion in exon 1.
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Key facts
- Cell type
- HEK-293T
- Species or organism
- Human
- Tissue
- Kidney
- Form
- Liquid
- Knockout validation
- Sanger Sequencing
- Mutation description
- Knockout achieved by using CRISPR/Cas9, Homozygous: 215 bp insertion in exon 1
Alternative names
CG1I, Cyclin G1 binding protein 1, ZNFN4A1, Zinc finger HIT domain containing protein 1, Zinc finger protein subfamily 4A member 1, p18 Hamlet, zinc finger, HIT type 1
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ZNHIT1 KO cell line available to order. Free of charge wild type control provided. Knockout achieved by using CRISPR/Cas9, Homozygous: 215 bp insertion in exon 1.
Key facts
- Cell type
- HEK-293T
- Species or organism
- Human
- Tissue
- Kidney
- Form
- Liquid
- Knockout validation
- Sanger Sequencing
- Mutation description
- Knockout achieved by using CRISPR/Cas9, Homozygous: 215 bp insertion in exon 1
- Concentration
Properties
- Gene name
- ZNHIT1
- Gene editing type
- Knockout
- Gene editing method
- CRISPR technology
- Knockout validation
- Sanger Sequencing
- Zygosity
- Homozygous
Quality control
- STR analysis
- CSF1PO, D13S317, D7S820, D5S818, TH01, D16S539, TPOX
Cell culture
- Biosafety level
- EU: 2 US: 2
- Adherent/suspension
- Adherent
- Gender
- Female
Handling procedures
- Initial handling guidelines
Upon arrival, the vial should be stored in liquid nitrogen vapor phase and not at -80°C. Storage at -80°C may result in loss of viability.
1. Thaw the vial in 37°C water bath for approximately 1-2 minutes.
2. Transfer the cell suspension (0.8 mL) to a 15 mL/50 mL conical sterile polypropylene centrifuge tube containing 8.4 mL pre-warmed culture medium, wash vial with an additional 0.8 mL culture medium (total volume 10 mL) to collect remaining cells, and centrifuge at 201 x g (rcf) for 5 minutes at room temperature. 10 mL represents minimum recommended dilution. 20 mL represents maximum recommended dilution.
3. Resuspend the cell pellet in 5 mL pre-warmed culture medium and count using a haemocytometer or alternative cell counting method seed all remaining cells into a T25.
4. Incubate the culture at 37°C incubator with 5% CO2. Check the culture one day after revival and continue to check until 80% confluent. Media change can be given if needed.
5. Once confluent passage into an appropriate flask at a density of 2x104 cells/cm2. Seeding density is given as a guide only and should be scaled to align with individual lab schedules. Cultures should be monitored daily.- Subculture guidelines
- All seeding densities should be based on cell counts gained by established methods.
- A guide seeding density of 2x104 cells/cm2 is recommended.
- Cells should be passaged when they have achieved 80-90% confluence.
- Culture medium
- DMEM (High Glucose) + 10% FBS
- Cryopreservation medium
- Cell Freezing Medium-DMSO Serum free media, contains 8.7% DMSO in MEM supplemented with methyl cellulose.
Storage
- Shipped at conditions
- Dry Ice
- Appropriate short-term storage conditions
- -196°C
- Appropriate long-term storage conditions
- -196°C
Notes
We will provide viable cells that proliferate on revival.
This product is subject to limited use licenses from The Broad Institute, ERS Genomics Limited and Sigma-Aldrich Co. LLC, and is developed with patented technology. For full details of the licenses and patents please refer to our limited use license and patent pages.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
ZNHIT1 also known as Zinc finger HIT-type containing 1 is a protein that plays a role in the assembly of multiprotein complexes. It has a molecular mass of approximately 29 kDa. This protein is found expressed in various tissues including the brain heart and liver suggesting its involvement in a range of biological functions. ZNHIT1 contains zinc finger motifs which are key for its interaction with other proteins facilitating its mechanical role in cellular processes.
- Biological function summary
The interaction of ZNHIT1 with other protein units forms part of the histone and transcription machinery. It is a component of the U3 small nucleolar ribonucleoprotein (snoRNP) complex which is essential for the biogenesis of ribosomes. Through its zinc finger domains ZNHIT1 interacts with proteins such as NOLC1 aiding in the functional assembly of ribosome-related structures. The activity of ZNHIT1 within this context points to its importance in maintaining protein synthesis and gene regulation.
- Pathways
ZNHIT1 is involved in the metabolic and cellular processes of ribosome assembly and gene expression. In the context of these pathways its interaction with NOLC1 facilitates alterations in nucleolar activity which can impact protein synthesis. ZNHIT1 can influence pathways such as the assembly of the pre-rRNA complex and is also linked to transcriptional regulation alongside proteins like SMARCC1 within epigenetic modification routes.
- Associated diseases and disorders
ZNHIT1 has connections with certain cancers and neurodegenerative conditions. Its regulatory roles in gene expression and ribosome production can contribute to pathologies like hepatocellular carcinoma where aberrations in protein synthesis are common. Altered function or expression of ZNHIT1 can also associate with neurological disorders interconnected through proteins such as AATF which is implicated in neuronal survival. Understanding ZNHIT1’s involvement in these pathways can offer insights into potential therapeutic strategies.
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1 product image
Sanger Sequencing - Human ZNHIT1 knockout HEK-293T cell line (ab266552)
Homozygous: 215 bp insertion in exon1
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