ioGlutamatergic Neurons WT (isogenic control) - Human iPSC derived cells available to order.
Note that this is suitable for studies with WT Glutamatergic Neurons and also serves as an isogenic control for disease models (HTT, other).
Images
Key facts
- Species or organism
- Human
- Form
- Liquid
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ioGlutamatergic Neurons WT (isogenic control) - Human iPSC derived cells available to order.
Note that this is suitable for studies with WT Glutamatergic Neurons and also serves as an isogenic control for disease models (HTT, other).
Key facts
- Species or organism
- Human
- Form
- Liquid
- Concentration
Cell culture
- Biosafety level
- EU: 1 US: 1
- Viability
- > 85%
- Gender
- Male
Storage
- Shipped at conditions
- Dry Ice
- Appropriate short-term storage conditions
- -196°C
- Appropriate long-term storage conditions
- -196°C
Notes
Note that this is suitable for studies with WT Glutamatergic Neurons and also serves as an isogenic control for disease models (HTT, other).
ioGlutamatergic Neurons have been reprogrammed from human induced pluripotent stem cells (iPSC) using opti-ox, a precise reprogramming technology. Human stem cells, within days, convert into consistent, mature, functional glutamatergic neurons providing a high quality human model for the study of neurological activity and disease.
ioGlutamatergic Neurons cultures consist mainly of glutamatergic neurons (>80%) characterised by the expression of the glutamate transporter genes VGLUT1 and VGLUT2 (see Figure 1). The minor remaining fraction of the neuronal population express marker genes of cholinergic neurons. A bulk RNA-seq analysis shows that ioGlutamatergic Neurons have a rostral CNS identity and express the classical cortical maker genes FOXG1 and TBR1 (data not shown).
In partnership with bit.bio
Karyotype: Normal
Seeding Density: 30,000 cells/cm2
Seeding compatibility: 6-, 12-, 24-, 96- and 384-well compatible
Quality control: ICC and gene expression analysis
Research applications: Academic research, Drug development, Neurotoxicology, Genetic screening (e.g. CRISPR screening).
This product is subject to limited use licenses from iPS Academia Japan Inc, TET Systems GmbH, ERS Genomics Limited and Sigma-Aldrich Co. LLC and is developed with Bit Bio patented technology. For full details of the licenses and patents please refer to our limited use license and patent pages.
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5 product images
Functional Studies - ioGlutamatergic Neurons WT (isogenic control) - Human iPSC derived cells (ab303447)
Day 1 to 11 post-thawing; 400X magnification; scale bar: 100µm.
Functional Studies - ioGlutamatergic Neurons WT (isogenic control) - Human iPSC derived cells (ab303447)
Array Wide Spike Detection Rate histograms (AWSDR – a graphical measure of synchrony) for 10-minute recordings on Day 8, 13 and 20 post-revival ioGlutamatergic Neurons in co-culture with primary rat-derived astrocytes. Results show prominent synchronicity on Day 13, exemplified by the ‘spikier’ nature of the associated AWSDR, which increases at Day 20. Cells were cultured in the recommended open-source medium and recorded on 64-electrode MEAs.
Functional Studies - ioGlutamatergic Neurons WT (isogenic control) - Human iPSC derived cells (ab303447)
(A) The graph shows the % of active bursting electrodes for each time point. (B) An example of a spontaneous spike, taken at Day 8 post-revival (1 second sweep, 32 µV/-18 µV). (C) An example of a bursting phenotype, taken at Day 20 post-revival (1 second sweep, 16 µV/-16 µV). Cells were cultured in the recommended open-source medium and recorded on 64-electrode MEAs.
Immunocytochemistry/ Immunofluorescence - ioGlutamatergic Neurons WT (isogenic control) - Human iPSC derived cells (ab303447)
Immunofluorescent staining on post-revival day 11 demonstrates homogenous expression of pan-neuronal proteins (MAP2 and TUBB3) and glutamatergic neuron-specific transporters (VGLUT1 and VGLUT2). Cells exhibit neurite outgrowth.
Functional Studies - ioGlutamatergic Neurons WT (isogenic control) - Human iPSC derived cells (ab303447)
Examples of MaxOne high-resolution multi electrode array (MEA) recordings of ioGlutamatergic Neurons in BrainPhys™ media. The activity maps show firing rate (A), spike amplitude (B) and % of active electrodes (C). Results demonstrate a time-dependent increase of spontaneous activity during neuronal maturation from 2 to 3 weeks post-revival.
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