ioOligodendrocyte-like cells™ - Human iPSC derived cells
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- LM
Supplier Data
Light Microscopy - ioOligodendrocyte-like cells™ - Human iPSC derived cells (AB317487)
Cells show an oligodendrocyte-like morphology by day 8. Upon reprogramming, cells show rapid morphological changes, acquiring an OPC-like morphology by day 1 post-revival. By day 8, cells have matured and display an oligodendrocyte-like morphology. Brightfield images show day 1 and day 8 post-thawing; scale bar : 400 um.
- ICC/IF
Supplier Data
Immunocytochemistry/ Immunofluorescence - ioOligodendrocyte-like cells™ - Human iPSC derived cells (AB317487)
ioOligodendrocyte-like cells express oligodendroglial-specific markers. Immunofluorescent staining of the cells at day 1 (upper panel) and day 8 (lower panel) post-revival. At day 1, the cells are positive for the oligodendrocyte-specific marker O4 (red), and the DAPI counterstain (blue). At day 8, ioOligodendrocyte-like cells show an increased complexity and are positive for O4 (red), the myelin basic protein (MBP) (green), and the DAPI counterstain (blue). 100X magnification; scale bar : 100 um.
- Sequencing
Supplier Data
Sequencing - ioOligodendrocyte-like cells™ - Human iPSC derived cells (AB317487)
Whole transcriptome analysis demonstrates high lot-to-lot consistency of ioOligodendrocyte-like cells. Bulk RNA-sequencing analysis was performed on three different lots of manufactured product at day 1 and day 8 post revival. Principal component analysis (PCA) represents the variance in gene expression between the three different lots of ioOligodendrocyte-like cells. This analysis shows lots clustering very closely which demonstrates high consistency at each given timepoint. This lot-to-lot consistency in every vial gives scientists confidence in their experimental reproducibility. Colours represent the parental non-induced hiPSC cell line and the three lots of ioOligodendrocyte-like cells; shapes represent different timepoints.
- RT-PCR
Supplier Data
RT-PCR - ioOligodendrocyte-like cells™ - Human iPSC derived cells (AB317487)
Key oligodendroglial genes are expressed by ioOligodendrocyte-like cells. Following reprogramming, the cells downregulate expression of the pluripotency gene OCT4, whilst demonstrating robust expression of relevant oligodendroglial markers, including PDGFRA, PLP1, MBP, CNP, MAG, and MYRF. Gene expression levels assessed by RT-qPCR, data expressed relative to the reference (housekeeping) gene, HMBS. Data represents day 1 and day 8 post-revival samples; n=2 technical replicates.
Reactivity data
Product details
ioOligodendrocyte-like cells are precision reprogrammed from human iPSC using opti-ox™ technology, meaning scalability and consistency are built-in.
Upon thawing (day 1), cells are ready for experimentation and express the oligodendroglial lineage marker O4. By simply maintaining cells in a monolayer culture, cells rapidly mature and acquire a typical oligodendrocyte-like morphology with multiple branched processes. Cells start expressing oligodendrocyte markers, including MBP, PLP1, CNP and MAG.
Properties and storage information
Shipped at conditions
Appropriate short-term storage conditions
Appropriate long-term storage conditions
Handling procedures
Culture medium
Refer to protocol booklet
Cryopreservation medium
Cell Freezing Medium-DMSO Serum free media, contains 8.7% DMSO in MEM supplemented with methyl cellulose.
Cell culture
Biosafety level
EU: 1 US: 1
Gender
Male
Viability
> 80%
Product protocols
- Visit the General protocols
- Visit the Troubleshooting
- Download websiteProtocolBooklet|en
Product promise
Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.
For licensing inquiries, please contact partnerships@abcam.com