ioOligodendrocyte-like cells™ - Human iPSC derived cells available to order.
ioOligodendrocyte-like cells are highly characterised cells that resemble a pre-myelinating oligodendrocyte state.
Images
Key facts
- Species or organism
- Human
- Form
- Liquid
ioOligodendrocyte-like cells™ - Human iPSC derived cells available to order.
ioOligodendrocyte-like cells are highly characterised cells that resemble a pre-myelinating oligodendrocyte state.
Key facts
- Species or organism
- Human
- Form
- Liquid
- Concentration
Cell culture
- Biosafety level
- EU: 1 US: 1
- Viability
- > 80%
- Gender
- Male
Handling procedures
- Culture medium
- Refer to protocol booklet
- Cryopreservation medium
- Cell Freezing Medium-DMSO Serum free media, contains 8.7% DMSO in MEM supplemented with methyl cellulose.
Storage
- Shipped at conditions
- Dry Ice
- Appropriate short-term storage conditions
- -196°C
- Appropriate long-term storage conditions
- -196°C
Notes
ioOligodendrocyte-like cells are highly characterised cells that resemble a pre-myelinating oligodendrocyte state. These cells enable the screening of compounds that modulate myelination, supporting drug development for neurodegenerative and demyelinating diseases, such as multiple sclerosis.
ioOligodendrocyte-like cells are precision reprogrammed from human iPSC using opti-ox™ technology, meaning scalability and consistency are built-in.
Upon thawing (day 1), cells are ready for experimentation and express the oligodendroglial lineage marker O4. By simply maintaining cells in a monolayer culture, cells rapidly mature and acquire a typical oligodendrocyte-like morphology with multiple branched processes. Cells start expressing oligodendrocyte markers, including MBP, PLP1, CNP and MAG.
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4 product images
Sequencing - ioOligodendrocyte-like cells™ - Human iPSC derived cells (ab317487)
Whole transcriptome analysis demonstrates high lot-to-lot consistency of ioOligodendrocyte-like cells.
Bulk RNA-sequencing analysis was performed on three different lots of manufactured product at day 1 and day 8 post revival. Principal component analysis (PCA) represents the variance in gene expression between the three different lots of ioOligodendrocyte-like cells. This analysis shows lots clustering very closely which demonstrates high consistency at each given timepoint. This lot-to-lot consistency in every vial gives scientists confidence in their experimental reproducibility. Colours represent the parental non-induced hiPSC cell line and the three lots of ioOligodendrocyte-like cells; shapes represent different timepoints.
Light Microscopy - ioOligodendrocyte-like cells™ - Human iPSC derived cells (ab317487)
Cells show an oligodendrocyte-like morphology by day 8.
Upon reprogramming, cells show rapid morphological changes, acquiring an OPC-like morphology by day 1 post-revival. By day 8, cells have matured and display an oligodendrocyte-like morphology. Brightfield images show day 1 and day 8 post-thawing; scale bar: 400 um.
RT-PCR - ioOligodendrocyte-like cells™ - Human iPSC derived cells (ab317487)
Key oligodendroglial genes are expressed by ioOligodendrocyte-like cells.
Following reprogramming, the cells downregulate expression of the pluripotency gene OCT4, whilst demonstrating robust expression of relevant oligodendroglial markers, including PDGFRA, PLP1, MBP, CNP, MAG, and MYRF. Gene expression levels assessed by RT-qPCR, data expressed relative to the reference (housekeeping) gene, HMBS. Data represents day 1 and day 8 post-revival samples; n=2 technical replicates.
Immunocytochemistry/ Immunofluorescence - ioOligodendrocyte-like cells™ - Human iPSC derived cells (ab317487)
ioOligodendrocyte-like cells express oligodendroglial-specific markers.
Immunofluorescent staining of the cells at day 1 (upper panel) and day 8 (lower panel) post-revival. At day 1, the cells are positive for the oligodendrocyte-specific marker O4 (red), and the DAPI counterstain (blue). At day 8, ioOligodendrocyte-like cells show an increased complexity and are positive for O4 (red), the myelin basic protein (MBP) (green), and the DAPI counterstain (blue). 100X magnification; scale bar: 100 um.
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Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.
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