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Mouse wild-type RAW 264.7 cell line is not available for individual purchase. This product can be added free of charge to a KO cell line order of the same background.

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Key facts

Cell type

RAW 264.7

Species or organism

Mouse

Tissue

Lymphatic

Form

Liquid

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Mouse wild-type RAW 264.7 cell line is not available for individual purchase. This product can be added free of charge to a KO cell line order of the same background.

Key facts

Cell type

RAW 264.7

Form

Liquid

Disease

Carcinoma

Concentration
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Cell culture

Biosafety level

EU: 2 US: 2

Adherent/suspension

Adherent

Gender

Male

Handling procedures

Initial handling guidelines

Upon arrival, the vial should be stored in liquid nitrogen vapor phase and not at -80°C. Storage at -80°C may result in loss of viability.

1. Thaw the vial in 37°C water for bath approximately 1-2 minutes.
2. Transfer the cell suspension (0.8 mL) to a 15 mL/50 mL conical sterile polypropylene centrifuge tube containing 8.4 mL pre-warmed culture medium, wash vial with an additional 0.8 mL culture medium (total volume 10 mL) to collect remaining cells, and centrifuge at 201 x g (rcf) for 5 minutes at room temperature. 10 mL represents minimum recommended dilution. 20 mL represents maximum recommended dilution.
3. Resuspend the cell pellet in 5 mL pre-warmed culture medium and count using a haemocytometer or alternative cell counting method all remaining cells into an ultra-low attachment T25.
4. Incubate the culture at 37°C incubator with 5% CO2. Check the culture one day after revival and continue to check until 80% confluent.
5. Once confluent passage into an appropriate flask at a density of 2x105 cells/cm2. Seeding density is given as a guide only and should be scaled to align with individual lab schedules. Cultures should be monitored daily.

Subculture guidelines

  • Grow in ultra-low attachment flasks.

  • Do not wash or use dissociation reagent. Cells are semi-adherent. Tap flask sharply to remove adhered cells.

  • All seeding densities should be based on cell counts gained by established methods.

  • A guide seeding density of 2x105 cells/mL is recommended.

  • Cells should be passaged when they have achieved 80-90% confluence.

Culture medium

DMEM + 10% FBS

Cryopreservation medium

Cell Freezing Medium-DMSO Serum free media, contains 8.7% DMSO in MEM supplemented with methyl cellulose.

Storage

Shipped at conditions

Dry Ice

Appropriate long-term storage conditions

-196°C

Notes

Wild-type cell lines are sold with knockout cell lines only - not available for individual purchase.

We will provide viable cells that proliferate on revival.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

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    Product protocols

    For this product, it's our understanding that no specific protocols are required. You can:

    Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

    For licensing inquiries, please contact partnerships@abcam.com

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