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AB94080

Annexin-2/ANXA2 overexpression 293T lysate (whole cell)

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Annexin-2/ANXA2 overexpression 293T lysate (whole cell) suitable for WB. View our extensive range of validated lysates from normal and diseased human, mouse and rat tissue.

View Alternative Names

ANX 2, ANX2L4, ANXA2_HUMAN, Annexin A2, Annexin II, Annexin II, heavy chain, Annexin-2, Arylsulfatase B, CAL1H, Calpactin I heavy chain, Calpactin I heavy polypeptide, Calpactin-1 heavy chain, Chromobindin-8, Epididymis secretory protein Li 270, HEL S 270, LIP2, LPC2, LPC2D, Lipocortin II, P36 protein, PAP-IV, Placental anticoagulant protein IV, Protein I, calpactin I heavy polypeptide (p36), p36

3 Images
Western blot - Annexin-2/ANXA2 overexpression 293T lysate (whole cell) (AB94080)
  • WB

Unknown

Western blot - Annexin-2/ANXA2 overexpression 293T lysate (whole cell) (AB94080)

The ESAM protein has a predicted molecular weight of 41 kDa. The first 29 amino acids of the ESAM sequence act as a signal sequence. The band at 37 kDa may represent the cleaved form of the ESAM protein.

All lanes:

Western blot - Anti-ESAM antibody (<a href='/en-us/products/primary-antibodies/esam-antibody-ab74777'>ab74777</a>) at 1 µg/mL

Lane 1:

Human heart tissue lysate - total protein (<a href='/en-us/products/unavailable/human-heart-tissue-lysate-total-protein-ab29431'>ab29431</a>) at 10 µg

Lane 2:

Human liver tissue lysate - total protein (<a href='/en-us/products/unavailable/human-liver-tissue-lysate-total-protein-ab29889'>ab29889</a>) at 10 µg

Lane 3:

Human kidney tissue lysate - total protein (<a href='/en-us/products/unavailable/human-kidney-tissue-lysate-total-protein-ab30203'>ab30203</a>) at 10 µg

Lane 4:

HepG2 (Human hepatocellular liver carcinoma cell line) Whole Cell Lysate at 10 µg

Lane 5:

HEK293 (Human embryonic kidney cell line) Whole Cell Lysate at 10 µg

Lane 6:

Lung (Human) Tissue Lysate at 10 µg

Predicted band size: 41 kDa

Observed band size: 100 kDa,15 kDa,250 kDa,37 kDa,41 kDa

true

Exposure time: 3min

Western blot - Annexin-2/ANXA2 overexpression 293T lysate (whole cell) (AB94080)
  • WB

Unknown

Western blot - Annexin-2/ANXA2 overexpression 293T lysate (whole cell) (AB94080)

false

SDS-PAGE - Annexin-2/ANXA2 overexpression 293T lysate (whole cell) (AB94080)
  • SDS-PAGE

Unknown

SDS-PAGE - Annexin-2/ANXA2 overexpression 293T lysate (whole cell) (AB94080)

ab94080 at 15μg/lane on an SDS-PAGE gel.

Key facts

Cell type

HEK-293T

Species or organism

Human

Form

Liquid

form

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Complementary Products

Proteins & Peptides

AB93005

Recombinant Human Annexin-2/ANXA2 protein (6x His tag N-Terminus)

Western blot - Recombinant Human Annexin-2/ANXA2 protein (6x His tag N-Terminus) (AB93005)

  • 5
  • 2
Primary Antibodies

AB178677

Anti-Annexin-2/ANXA2 antibody [EPR13052(B)]

RabMAb|Recombinant

Flow Cytometry (Intracellular) - Anti-Annexin-2/ANXA2 antibody [EPR13052(B)] (AB178677)

  • 0
  • 0
Cell Lines & Lysates

AB166833

Hep G2 whole cell lysate

  • 0
  • 0
Primary Antibodies

AB110304

Anti-SIRT1 antibody [19A7AB4]

BOND RX™ Validated|KO Validated|Recombinant

Western blot - Anti-SIRT1 antibody [19A7AB4] (AB110304)

  • 5
  • 26
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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "WB": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Product details

ab94080 is a 293T cell transfected lysate in which Human Annexin II has been transiently over-expressed using a pCMV-Annexin II plasmid. The lysate is provided in 1X Sample Buffer.
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Properties and storage information

Shipped at conditions
Dry Ice
Appropriate short-term storage conditions
-20°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Annexin-2 also known as ANXA2 is a multifunctional protein of approximately 36 kDa. It belongs to the annexin family and is known for its strong calcium-dependent interactions with phospholipid membranes. Commonly expressed in endothelial cells it also appears in various tissues including epithelial cells and certain types of cancer cells. ANXA2 forms a heterotetramer complex with S100A10 also known as p11 which modulates its availability and function. Other names for ANXA2 include its gene designation M298 and its role in complexes often goes by ANXA2R.
Biological function summary

Annexin-2 acts beyond mere cellular structural support. It plays a part in fibrinolysis by acting as a co-receptor for plasminogen and tissue plasminogen activator promoting plasmin generation. The protein is also implicated in membrane trafficking signal transduction and organization of microdomains on cellular surfaces. As part of a complex with S100A10 ANXA2 can bind to cytoskeletal elements facilitating vesicle trafficking and influencing cell shape and motility. This complex assembly hints at ANXA2's importance in maintaining cellular dynamism and interplay with external signals.

Pathways

ANXA2 is integral in the regulation of the plasminogen activation pathway important for fibrinolytic activity. It also participates in the signal transduction pathways influencing cellular responses to stress and growth factors. ANXA2 interacts with various proteins like Src kinase and is involved in processes like the EGFR signaling pathway. This protein often crosstalks with the cytoskeletal network enabling the coupling of membrane dynamics with intracellular signaling cascades.

Annexin-2's dysregulation associates with conditions such as cancer and thrombosis. In cancer overexpression of ANXA2 links with tumor progression invasion and metastasis acting synergistically with proteins like Src to promote oncogenic pathways. In thrombosis ANXA2’s role in fibrinolysis becomes impaired contributing to excessive clot formation. Studies also indicate interactions with plasminogen-related proteins as being central to understanding its pathological roles in these diseases.
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Cell culture

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Product protocols

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Product promise

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