AB275045

Apoptosis pathway knockout cell lysates panel

Name: Apoptosis pathway knockout cell lysates panel (ab275045) | Abcam
Brand: Abcam Limited
SKU: AB275045

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Apoptosis pathway knockout cell lysates panel suitable for WB. View our extensive range of validated lysates from normal and diseased human, mouse and rat tissue.

View Alternative Names

BCL2L4, BAX, Apoptosis regulator BAX, Bcl-2-like protein 4, Bcl2-L-4, CD262, DR5, KILLER, TRAILR2, TRICK2, ZTNFR9, UNQ160/PRO186, TNFRSF10B, Tumor necrosis factor receptor superfamily member 10B, Death receptor 5, TNF-related apoptosis-inducing ligand receptor 2, TRAIL receptor 2, TRAIL-R2, MORT1, GIG3, FADD, FAS-associated death domain protein, FAS-associating death domain-containing protein, Growth-inhibiting gene 3 protein, Mediator of receptor induced toxicity, Proliferation marker protein Ki-67, Antigen identified by monoclonal antibody Ki-67, Antigen KI-67, Antigen Ki67, MKI67, CD95, APT1, FAS1, TNFRSF6, FAS, Tumor necrosis factor receptor superfamily member 6, Apo-1 antigen, Apoptosis-mediating surface antigen FAS, FASLG receptor, HSP27, HSP28, HSPB1, Heat shock protein beta-1, HspB1, 28 kDa heat shock protein, Estrogen-regulated 24 kDa protein, Heat shock 27 kDa protein, Heat shock protein family B member 1, Stress-responsive protein 27, HSP 27, SRP27, MCH5, CASP8, Caspase-8, CASP-8, Apoptotic cysteine protease, Apoptotic protease Mch-5, CAP4, FADD-homologous ICE/ced-3-like protease, FADD-like ICE, ICE-like apoptotic protease 5, MORT1-associated ced-3 homolog, FLICE, MACH, BING2, DAP6, DAXX, Death domain-associated protein 6, Daxx, ETS1-associated protein 1, Fas death domain-associated protein, hDaxx, EAP1, Bcl-XL-binding protein v68, Phosphoglycerate mutase family member 5, PGAM5, CARDIAK, RICK, RIP2, UNQ277/PRO314/PRO34092, RIPK2, Receptor-interacting serine/threonine-protein kinase 2, CARD-containing interleukin-1 beta-converting enzyme-associated kinase, RIP-like-interacting CLARP kinase, Receptor-interacting protein 2, Tyrosine-protein kinase RIPK2, CARD-containing IL-1 beta ICE-kinase, RIP-2, BRCC45, BRE, BABAM2, BRISC and BRCA1-A complex member 2, BRCA1-A complex subunit BRE, BRCA1/BRCA2-containing complex subunit 45, Brain and reproductive organ-expressed protein, KIAA0901, JM21, HDAC6, Protein deacetylase HDAC6, E3 ubiquitin-protein ligase HDAC6, Tubulin-lysine deacetylase HDAC6

16 Images

Lab

Western blot - Apoptosis pathway knockout cell lysates panel (AB275045)

Lane 1 : Wild-type HeLa cell lysate (20 μg)

Lane 2 : FADD knockout HeLa cell lysate (20 μg)

Lane 3 : A431 cell lysate (20 μg)

Lane 4 : Jurkat cell lysate (20 μg)

Lanes 1-4 : Merged signal (red and green). Green - ab119059 observed at 25 kDa. Red - loading control ab181602 observed at 37 kDa.

ab119059 Anti-FADD antibody [OTI1C11] was shown to specifically react with FADD in wild-type HeLa cells. Loss of signal was observed when knockout cell line ab261817 (knockout cell lysate ab257261) was used. Wild-type and FADD knockout samples were subjected to SDS-PAGE. ab119059 and Anti-GAPDH antibody[EPR16891] - Loading Control (ab181602) were incubated overnight at 4°C at 1 in 2000 dilution and 1 in 20000 dilution respectively. Blots were developed with Goat anti-Mouse IgG H&L (IRDye^® 800CW) preadsorbed (ab216772) and Goat Anti-Rabbit IgG H&L (IRDye^® 680RD) preadsorbed (ab216777) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.

All lanes:

Western blot - Anti-FADD antibody [OTI1C11] (<a href='/en-us/products/primary-antibodies/fadd-antibody-oti1c11-ab119059'>ab119059</a>) at 1/2000 dilution

Lane 1:

Wild-type HeLa cell lysate at 20 µg

Lane 2:

FADD knockout HeLa cell lysate at 20 µg

Lane 2:

Western blot - Human FADD knockout HeLa cell line (<a href='/en-us/products/cell-lines/human-fadd-knockout-hela-cell-line-ab261817'>ab261817</a>)

Lane 3:

A431 cell lysate at 20 µg

Lane 4:

Jurkat cell lysate at 20 µg

Secondary

All lanes:

Western blot - Goat anti-Mouse IgG H&L (IRDye® 800CW) preadsorbed (<a href='/en-us/products/secondary-antibodies/goat-mouse-igg-h-l-irdye-800cw-preadsorbed-ab216772'>ab216772</a>) at 1/20000 dilution

Predicted band size: 23 kDa

Observed band size: 25 kDa

false

Lab

Western blot - Apoptosis pathway knockout cell lysates panel (AB275045)

Lane 1 : Wild-type HeLa cell lysate (20μg)

Lane 2 : CASP8 knockout HeLa cell lysate (20μg)

Lane 3 : Jurkat cell lysate (20μg)

Lane 4 : SH-SY5Y cell lysate (20μg)

Lanes 1- 4 : Merged signal (red and green). Green - ab32125 observed at 55 kDa. Red - loading control, ab8245 observed at 37 kDa.

ab32125 Anti-Caspase-8 antibody [E6] was shown to specifically react with Caspase-8 in wild-type HeLa cells in western blot. Loss of signal was observed when knockout cell line ab264958 (knockout cell lysate ab256857) was used. Wild-type and Caspase-8 knockout samples were subjected to SDS-PAGE. Membrane was blocked for 1 hour at room temperature in 0.1% TBST with 3% non-fat dried milk. ab32125 and Anti-GAPDH antibody [6C5] - Loading Control (ab8245) were incubated overnight at 4^°C at 1 in 3000 and 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye^® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye^® 680RD) preadsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.

All lanes:

Western blot - Anti-Caspase-8 antibody [E6] (<a href='/en-us/products/primary-antibodies/caspase-8-antibody-e6-ab32125'>ab32125</a>) at 1/3000 dilution

Lane 1:

Wild-type HeLa cell lysate at 20 µg

Lane 2:

CASP8 knockout HeLa cell lysate at 20 µg

Lane 2:

Western blot - Human CASP8 (Caspase-8) knockout HeLa cell line (<a href='/en-us/products/cell-lines/human-casp8-caspase-8-knockout-hela-cell-line-ab264958'>ab264958</a>)

Lane 3:

Jurkat cell lysate at 20 µg

Lane 4:

SH-SY5Y cell lysate at 20 µg

Predicted band size: 55 kDa

Observed band size: 55 kDa

false

Lab

Western blot - Apoptosis pathway knockout cell lysates panel (AB275045)

Lane 1 : Wild-type HeLa cell lysate (20μg)
Lane 2 : CASP8 knockout HeLa cell lysate (20μg)
Lane 3 : Jurkat cell lysate (20μg)
Lane 4 : SH-SY5Y cell lysate (20μg)
Lanes 1- 4 : Merged signal (red and green). Green - ab32397 observed at 55 kDa. Red - loading control, ab8245 observed at 37 kDa.
ab32397 Rabbit monoclonal [EPR2418Y] to IRF3 was shown to specifically react with Caspase-8 in wild-type HeLa cells in western blot. Loss of signal was observed when knockout cell line ab264958 (knockout cell lysate ab256857) was used. Wild-type and Caspase-8 knockout samples were subjected to SDS-PAGE. Membrane was blocked for 1 hour at room temperature in 0.1% TBST with 3% non-fat dried milk. ab32397 and Anti-GAPDH antibody [6C5] - Loading Control (ab8245) were incubated overnight at 4^°C at 1 in 500 and 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye^® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye^® 680RD) preadsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.

All lanes:

Western blot - Anti-Caspase-8 antibody [E7] (<a href='/en-us/products/primary-antibodies/caspase-8-antibody-e7-ab32397'>ab32397</a>) at 1/500 dilution

Lane 1:

Wild-type HeLa cell lysate at 20 µg

Lane 2:

CASP8 knockout HeLa cell lysate at 20 µg

Lane 2:

Western blot - Human CASP8 (Caspase-8) knockout HeLa cell line (<a href='/en-us/products/cell-lines/human-casp8-caspase-8-knockout-hela-cell-line-ab264958'>ab264958</a>)

Lane 3:

Jurkat cell lysate at 20 µg

Lane 4:

SH-SY5Y cell lysate at 20 µg

Predicted band size: 55 kDa

Observed band size: 55 kDa

false

Lab

Western blot - Apoptosis pathway knockout cell lysates panel (AB275045)

Lane 1 : Wild-type HeLa cell lysate (20 μg)

Lane 2 : PGAM5 knockout HeLa cell lysate (20 μg)

Lane 3 : HepG2 cell lysate (20 μg)

Lane 4 : Daudi cell lysate (20 μg)

Lanes 1-4 : Merged signal (red and green). Green - ab244218 observed at 32 kDa. Red - loading control ab52866 observed at 50 kDa.

ab244218 Anti-PGAM5 antibody [CL0624] was shown to specifically react with PGAM5 in wild-type HeLa cells. Loss of signal was observed when knockout cell line ab265141 (knockout cell lysate ab257581) was used. Wild-type and PGAM5 knockout samples were subjected to SDS-PAGE. ab244218 and Anti-alpha Tubulin antibody [EP1332Y] - Microtubule Marker (ab52866) were incubated overnight at 4°C at 1 and 1 in 20000 dilution respectively. Blots were developed with Goat anti-Mouse IgG H&L (IRDye^® 800CW) preadsorbed (ab216772) and Goat Anti-Rabbit IgG H&L (IRDye^® 680RD) preadsorbed (ab216777) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.

All lanes:

Western blot - Anti-PGAM5 antibody [CL0624] (<a href='/en-us/products/primary-antibodies/pgam5-antibody-cl0624-ab244218'>ab244218</a>) at 1 µg/mL

Lane 1:

Wild-type HeLa lysate at 20 µg

Lane 2:

PGAM5 knockout HeLa lysate at 20 µg

Lane 2:

Western blot - Human PGAM5 knockout HeLa cell line (<a href='/en-us/products/cell-lines/human-pgam5-knockout-hela-cell-line-ab265141'>ab265141</a>)

Lane 3:

HepG2 lysate at 20 µg

Lane 4:

Daudi lysate at 20 µg

Predicted band size: 32 kDa

Observed band size: 32 kDa

false

Lab

Western blot - Apoptosis pathway knockout cell lysates panel (AB275045)

Lane 1 : Wild-type HeLa cell lysate (20 μg)

Lane 2 : BRE knockout HeLa cell lysate (20 μg)

Lane 3 : SH-SY5Y cell lysate (20 μg)

Lane 4 : Daudi cell lysate (20 μg)

Lanes 1-4 : Merged signal (red and green). Green - ab177960 observed at 44 kDa. Red - loading control ab7291 observed at 50 kDa.

ab177960 Recombinant Anti-BRE antibody [EPR11858] was shown to specifically react with BRE in wild-type HeLa cells. Loss of signal was observed when knockout cell line ab264928 (knockout cell lysate ab257861) was used. Wild-type and BRE knockout samples were subjected to SDS-PAGE. ab177960 and Anti-alpha Tubulin antibody [DM1A] - Loading Control (ab7291) were incubated overnight at 4°C at 1 in 1000 dilution and 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye^® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye^® 680RD) preadsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.

All lanes:

Western blot - Anti-BRCC45/BRE antibody [EPR11858] (<a href='/en-us/products/primary-antibodies/brcc45-bre-antibody-epr11858-ab177960'>ab177960</a>) at 1/1000 dilution

Lane 1:

Wild-type HeLa cell lysate at 20 µg

Lane 2:

BRCC45/BRE knockout HeLa cell lysate at 20 µg

Lane 2:

Western blot - Human BRE (BRCC45) knockout HeLa cell line (<a href='/en-us/products/cell-lines/human-bre-brcc45-knockout-hela-cell-line-ab264928'>ab264928</a>)

Lane 3:

SH-SY5Y cell lysate at 20 µg

Lane 4:

Daudi cell lysate at 20 µg

Secondary

All lanes:

Western blot - Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-irdye-800cw-preadsorbed-ab216773'>ab216773</a>) at 1/20000 dilution

Predicted band size: 44 kDa

Observed band size: 44 kDa

false

Lab

Western blot - Apoptosis pathway knockout cell lysates panel (AB275045)

Lane 1 : Wild-type HeLa cell lysate (20 μg)

Lane 2 : FAS knockout HeLa cell lysate (20 μg)

Lanes 1-2 : Merged signal (red and green). Green - ab133619 observed at 37 kDa. Red - loading control ab8245 observed at 37 kDa.

ab133619 Anti-Fas antibody [EPR5700] was shown to specifically react with Fas in wild-type HeLa cells. Loss of signal was observed when knockout cell line ab265260 (knockout cell lysate ab256911) was used. Wild-type and Fas knockout samples were subjected to SDS-PAGE. ab133619 and Anti-GAPDH antibody [6C5] - Loading Control (ab8245) were incubated overnight at 4°C at 1 in 1000 and 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye^® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye^® 680RD) preadsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.

All lanes:

Western blot - Anti-Fas antibody [EPR5700] (<a href='/en-us/products/primary-antibodies/fas-antibody-epr5700-ab133619'>ab133619</a>) at 1/1000 dilution

Lane 1:

Wild-type HeLa cell lysate at 20 µg

Lane 2:

Western blot - Human FAS knockout HeLa cell line (<a href='/en-us/products/cell-lines/human-fas-knockout-hela-cell-line-ab265260'>ab265260</a>)

Lane 2:

FAS knockout HeLa cell lysate at 20 µg

Predicted band size: 37 kDa

Observed band size: 37 kDa

false

Lab

Western blot - Apoptosis pathway knockout cell lysates panel (AB275045)

Lane 1 : Ramos cell lysate (20 μg)

Lane 2 : Wild-type HeLa cell lysate (20 μg)

Lane 3 : MKI67 knockout HeLa cell lysate (20 μg)

Lanes 1 - 3 : Merged signal (red and green). Green - ab16667 observed at 359 kDa. Red - loading control, ab130007 observed at 125 kDa.

ab16667 was shown to react with Ki67 in wild-type HeLa cells. Loss of signal was observed when knockout cell line ab255407 (knockout cell lysate ab263762) was used. Wild-type and Ki67 knockout samples were subjected to SDS-PAGE. ab16667 and Anti-Vinculin antibody [VIN-54] (ab130007) were incubated overnight at 4°C at 1 in 100 dilution and 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye^® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye^® 680RD) preadsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.

All lanes:

Western blot - Anti-Ki67 antibody [SP6] (<a href='/en-us/products/primary-antibodies/ki67-antibody-sp6-ab16667'>ab16667</a>) at 1/100 dilution

Lane 1:

Ramos cell lysate at 20 µg

Lane 2:

Wild-type HeLa cell lysate at 20 µg

Lane 3:

Western blot - Human MKI67 (Ki67) knockout HeLa cell line (<a href='/en-us/products/cell-lines/human-mki67-ki67-knockout-hela-cell-line-ab255407'>ab255407</a>) at 20 µg

Lane 3:

Western blot - Human MKI67 (Ki67) knockout HeLa cell lysate (<a href='/en-us/products/cell-lysates/human-mki67-ki67-knockout-hela-cell-lysate-ab263762'>ab263762</a>) at 20 µg

Secondary

All lanes:

Predicted band size: 358 kDa

Observed band size: 124 kDa,359 kDa

false

Lab

Western blot - Apoptosis pathway knockout cell lysates panel (AB275045)

Lane 1 : Wild-type HeLa cell lysate (20 μg)

Lane 2 : RIPK2 knockout HeLa cell lysate (20 μg)

Lane 3 : Ramos cell lysate (20 μg)

Lane 4 : Jurkat cell lysate (20 μg)

Lanes 1-4 : Merged signal (red and green). Green - ab75257 observed at 65 kDa. Red - loading control ab181602 observed at 37 kDa.

ab75257 Anti-RIP2 antibody [AF28D3] was shown to specifically react with RIP2 in wild-type HeLa cells. Loss of signal was observed when knockout cell line ab264688 (knockout cell lysate ab258636) was used. Wild-type and RIP2 knockout samples were subjected to SDS-PAGE. ab75257 and Anti-GAPDH antibody[EPR16891] - Loading Control (ab181602) were incubated overnight at 4°C at 1 in 2000 dilution and 1 in 20000 dilution respectively. Blots were developed with Goat anti-Mouse IgG H&L (IRDye^® 800CW) preadsorbed (ab216772) and Goat Anti-Rabbit IgG H&L (IRDye^® 680RD) preadsorbed (ab216777) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.

All lanes:

Western blot - Anti-RIP2 antibody [AF28D3] (<a href='/en-us/products/primary-antibodies/rip2-antibody-af28d3-ab75257'>ab75257</a>) at 1/2000 dilution

Lane 1:

Wild-type HeLa cell lysate at 20 µg

Lane 2:

RIPK2 knockout HeLa cell lysate at 20 µg

Lane 2:

Western blot - Human RIPK2 (RIP2) knockout HeLa cell line (<a href='/en-us/products/cell-lines/human-ripk2-rip2-knockout-hela-cell-line-ab264688'>ab264688</a>)

Lane 3:

Ramos cell lysate at 20 µg

Lane 4:

Jurkat cell lysate at 20 µg

Secondary

All lanes:

Predicted band size: 61 kDa

Observed band size: 65 kDa

false

Lab

Western blot - Apoptosis pathway knockout cell lysates panel (AB275045)

Lane 1 : Wild-type HeLa cell lysate (20 μg)

Lane 2 : FADD knockout HeLa cell lysate (20 μg)

Lane 3 : A431 cell lysate (20 μg)

Lane 4 : Jurkat cell lysate (20 μg)

Lanes 1-4 : Merged signal (red and green). Green - ab108601 observed at 25 kDa. Red - loading control ab8245 observed at 37 kDa.

ab108601 Anti-FADD antibody [EPR4415] was shown to specifically react with FADD in wild-type HeLa cells. Loss of signal was observed when knockout cell line ab261817 (knockout cell lysate ab257261) was used. Wild-type and FADD knockout samples were subjected to SDS-PAGE. ab108601 and Anti-GAPDH antibody [6C5] - Loading Control (ab8245) were incubated overnight at 4°C at 1 in 1000 dilution and 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye^® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye^® 680RD) preadsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.

false

Lab

Western blot - Apoptosis pathway knockout cell lysates panel (AB275045)

Lane 1 : Wild-type HeLa cell lysate (20μg)
Lane 2 : TNFRSF10B knockout HeLa cell lysate (20μg)
Lanes 1- 2 : Merged signal (red and green). Green - ab199357 observed at 47 kDa. Red - loading control ab8245 observed at 37 kDa.
ab199357 Anti-DR5 antibody [EPR19310] was shown to specifically react with DR5 in wild-type HeLa cells in western blot. Loss of signal was observed when knockout cell line ab264922 (knockout cell lysate ab257748) was used. Wild-type and DR5 knockout samples were subjected to SDS-PAGE. Membrane was blocked for 1 hour at room temperature in 0.1% TBST with 3% non-fat dried milk. ab199357 and Anti-GAPDH antibody [6C5] - Loading Control (ab8245) were incubated overnight at 4^°C at 1 in 1000 dilution and 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye^® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye^® 680RD) preadsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.

false

Lab

Western blot - Apoptosis pathway knockout cell lysates panel (AB275045)

Lane 1 : Wild-type HeLa cell lysate (20μg)
Lane 2 : BAX knockout HeLa cell lysate (20μg)
Lanes 1- 2 : Merged signal (red and green). Green - ab32503 observed at 21 kDa. Red - loading control ab8245 observed at 37 kDa.
ab32503 Recombinant Anti-Bax antibody [E63] was shown to specifically react with BAX in wild-type HeLa cells in western blot. Loss of signal was observed when knockout cell line ab255363 (knockout cell lysate ab263841) was used. Wild-type and BAX knockout samples were subjected to SDS-PAGE. Membrane was blocked for 1 hour at room temperature in 0.1% TBST with 3% non-fat dried milk. ab32503 and Anti-GAPDH antibody [6C5] - Loading Control (ab8245) were incubated overnight at 4^°C at 1 in 1000 dilution and 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye^® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye^® 680RD) preadsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.

false

Lab

Western blot - Apoptosis pathway knockout cell lysates panel (AB275045)

Lane 1 : Wild-type HeLa cell lysate (20μg)
Lane 2 : DAXX knockout HeLa cell lysate (20μg)
Lanes 1- 2 : Merged signal (red and green). Green - ab32140 observed at 100 kDa. Red - loading control ab8245 observed at 37 kDa.
ab32140 Recombinant Anti-Daxx antibody [E94] was shown to specifically react with Daxx in wild-type HeLa cells in western blot. Loss of signal was observed when knockout cell line ab265233 (knockout cell lysate ab257408) was used. Wild-type and Daxx knockout samples were subjected to SDS-PAGE. Membrane was blocked for 1 hour at room temperature in 0.1% TBST with 3% non-fat dried milk. ab32140 and Anti-GAPDH antibody [6C5] - Loading Control (ab8245) were incubated overnight at 4^°C at 1 in 5000 dilution and 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye^® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye^® 680RD) preadsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.

false

Lab

Western blot - Apoptosis pathway knockout cell lysates panel (AB275045)

Lane 1 : Wild-type HeLa cell lysate (20μg)
Lane 2 : HDAC6 knockout HeLa cell lysate (20μg)
Lanes 1- 2 : Merged signal (red and green). Green - ab133493 observed at 160 kDa. Red - loading control ab8245 observed at 37 kDa.
ab133493 Anti-HDAC6 antibody [EPR1698(2)] was shown to specifically react with HDAC6 in wild-type HeLa cells in western blot. Loss of signal was observed when knockout cell line ab264804 (knockout cell lysate ab257145) was used. Wild-type and HDAC6 knockout samples were subjected to SDS-PAGE. Membrane was blocked for 1 hour at room temperature in 0.1% TBST with 3% non-fat dried milk. ab133493 and Anti-GAPDH antibody [6C5] - Loading Control (ab8245) were incubated overnight at 4^°C at 1 in 10000 dilution and 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye^® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye^® 680RD) preadsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.

false

Lab

Western blot - Apoptosis pathway knockout cell lysates panel (AB275045)

Lane 1 : Wild-type HeLa cell lysate (20μg)
Lane 2 : BAX knockout HeLa cell lysate (20μg)
Lanes 1- 2 : Merged signal (red and green). Green - ab182734 observed at 21 kDa. Red - loading control ab8245 observed at 37 kDa.
ab182734 Recombinant Anti-Bax antibody [EPR18284 was shown to specifically react with BAX in wild-type HeLa cells in western blot. Loss of signal was observed when knockout cell line ab255363 (knockout cell lysate ab263841) was used. Wild-type and BAX knockout samples were subjected to SDS-PAGE. Membrane was blocked for 1 hour at room temperature in 0.1% TBST with 3% non-fat dried milk. ab182734 and Anti-GAPDH antibody [6C5] - Loading Control (ab8245) were incubated overnight at 4^°C at 1 in 1000 dilution and 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye^® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye^® 680RD) preadsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.

false

Lab

Western blot - Apoptosis pathway knockout cell lysates panel (AB275045)

Lane 1 : Wild-type HeLa cell lysate (20μg)
Lane 2 : Hsp27 knockout HeLa cell lysate (20μg)
Lanes 1- 2 : Merged signal (red and green). Green - ab109376 observed at 23 kDa. Red - loading control ab8245 observed at 37 kDa.
ab109376 Anti-Hsp27 antibody [EPR5477] was shown to specifically react with Hsp27 in wild-type HeLa cells in western blot. Loss of signal was observed when knockout cell line ab261738 (knockout cell lysate ab256945) was used. Wild-type and Hsp27 knockout samples were subjected to SDS-PAGE. Membrane was blocked for 1 hour at room temperature in 0.1% TBST with 3% non-fat dried milk. ab109376 and Anti-GAPDH antibody [6C5] - Loading Control (ab8245) were incubated overnight at 4^°C at 1 in 1000 dilution and 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye^® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye^® 680RD) preadsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.

false

Lab

Western blot - Apoptosis pathway knockout cell lysates panel (AB275045)

Lane 1 : Wild-type HeLa cell lysate (20μg)
Lane 2 : Hsp27 knockout HeLa cell lysate (20μg)
Lanes 1- 2 : Merged signal (red and green). Green - ab62339 observed at 23 kDa. Red - loading control ab8245 observed at 37 kDa.
ab62339 Anti-Hsp27 antibody [EP1724Y] was shown to specifically react with Hsp27 in wild-type HeLa cells in western blot. Loss of signal was observed when knockout cell line ab261738 (knockout cell lysate ab256945) was used. Wild-type and Hsp27 knockout samples were subjected to SDS-PAGE. Membrane was blocked for 1 hour at room temperature in 0.1% TBST with 3% non-fat dried milk. ab62339 and Anti-GAPDH antibody [6C5] - Loading Control (ab8245) were incubated overnight at 4^°C at 1 in 500 dilution and 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye^® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye^® 680RD) preadsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.

false

Key facts

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left-column

style

left-column

Reactivity data

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style

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Product details

A collection of 12 knockout cell lysates of protein targets involved in the apoptotic cell death pathways (mitochondrial and death receptor), available together for your convenience, with matching parental cell lysates.

Find out more about our apoptosis assays.

Included in this panel:

CASP8 knockout HeLa cell lysate (ab256858)

FADD knockout HeLa cell lysate (ab257261)

BAX knockout HeLa cell lysate (ab263841)

PGAM5 knockout HeLa cell lysate (ab257581)

TNFRSF10B knockout HeLa cell lysate (ab257748)

HDAC6 knockout HeLa cell lysate (ab257145)

RIPK2 (RIP2) knockout HeLa cell lysate (ab258636)

FAS knockout HeLa cell lysate (ab256911)

DAXX knockout HeLa cell lysate (ab257408)

HSPB1 knockout HeLa cell lysate (ab256945)

BRE knockout HeLa cell lysate (ab257861)

MKI67 knockout HeLa cell lysate (ab263762)

Parts of the panels can be purchased separately. For further details please contact technical@abcam.com.

REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.

Lysate preparation: Our lysates are made using RIPA buffer to which we add a protease inhibitor cocktail and phosphatase inhibitor cocktail (ratio: 300:100:10). This means that the protein of interest is denatured. If you require a native form of the protein please use the live cell version. Please refer to our lysis protocol for further details on how our lysates are prepared.

User storage instructions: Lyophilizate may be stored at 4°C. After reconstitution, store at -20°C for short-term storage or -80°C for long-term storage.

This product is subject to limited use licenses from The Broad Institute, ERS Genomics Limited and Sigma-Aldrich Co. LLC, and is developed with patented technology. For full details of the licenses and patents please refer to our limited use license and patent pages.

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What's included?

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Properties and storage information

Shipped at conditions

Ambient - Can Ship with Ice

Appropriate short-term storage conditions

-20°C

Appropriate long-term storage conditions

-20°C

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The target proteins Ki67 DR5 FADD Bax Daxx Fas RIP2 Caspase-8 Hsp27 HDAC6 BRCC45/BRE and PGAM5 play various roles within cellular processes. Ki67 an antigen with a mass of about 395 kDa is a cellular marker for proliferation expressed in the cell nucleus during active phases of the cell cycle. DR5 (Death Receptor 5) part of the TNF receptor family is expressed on the cell membrane and engages in apoptosis pathways. FADD (Fas-Associated protein with Death Domain) acts as an adaptor molecule for death receptors. Bax a pro-apoptotic member of the Bcl-2 family is mainly located in the cytosol and mitochondria. Daxx (Death-domain associated protein) plays a role in apoptotic signaling and gene transcription modulation. Fas (CD95) often interacts with FADD to initiate apoptosis. RIP2 (Receptor-interacting serine/threonine-protein kinase 2) is involved in signaling pathways related to immune response. Caspase-8 executes apoptotic cell death and inflammation. Hsp27 (Heat-shock protein 27) assists in protein folding and apoptosis inhibition. HDAC6 a histone deacetylase participates in the modulation of protein acetylation. BRCC45/BRE is part of a complex that repairs DNA breaks. PGAM5 (Phosphoglycerate mutase family member 5) is a mitochondrial protein involved in metabolic processes.

Biological function summary

These proteins engage in cell regulation and apoptosis. Ki67 tracks cell proliferation important for tumor growth studies. DR5 often within receptor complexes triggers extrinsic apoptosis through ligand binding. FADD and Caspase-8 components of the death-inducing signaling complex (DISC) execute cell death. Bax promotes intrinsic apoptosis by permeabilizing mitochondrial membranes. Daxx modulates apoptosis and transcriptional repression. Fas engages FADD and Caspase-8 in apoptotic signaling. RIP2 participates in immune response signaling activating NF-kB pathway. Hsp27 a chaperone protein manages stress responses and inhibits apoptosis. HDAC6 orchestrates deacetylation of tubulin affecting cell mobility and stress response. BRCC45/BRE is mechanistically part of the BRCA1-A complex important for DNA repair. PGAM5 operates in dephosphorylation influencing apoptosis and antioxidant defense.

Pathways

These proteins integrate into apoptosis and immune response pathways. Ki67 does not partake directly in a classic pathway but indicates active cell division. DR5 Fas FADD and Caspase-8 participate in the extrinsic pathway of apoptosis vital for removing harmful cells. Bax integrates into the intrinsic apoptotic pathway a response to cellular stress. Daxx and RIP2 contribute to the JNK and NF-kB pathways respectively influencing apoptosis and inflammation. HDAC6 impacts the response to oxidative stress and cellular mobility pathways. BRCC45/BRE within BRCA1-A complex affects DNA damage response. PGAM5 plays roles in cellular stress response pathways integrating with related proteins such as Keap1.

These proteins have associations with cancer and neurodegenerative diseases. Abnormally increased expression of Ki67 often indicates aggressive tumors relating it to tumor progression. Dysregulation of DR5 Bax Fas FADD and Caspase-8 contributes to various cancers due to defective apoptosis. Daxx through its role in transcription regulation is linked to neurodegenerative disorders. RIP2's involvement in immune responses connects it to inflammatory diseases. HDAC6 by impacting protein folding and degradation ties into neurodegenerative diseases where misfolded proteins accumulate. BRCC45/BRE mutations affect DNA repair fidelity linking it to breast cancer. PGAM5 has implications in neurodegenerative diseases through its modulation of mitochondrial function.

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Product protocols

Visit the General protocols
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Product promise

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Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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Apoptosis pathway knockout cell lysates panel

Western blot - Apoptosis pathway knockout cell lysates panel (AB275045)

Western blot - Apoptosis pathway knockout cell lysates panel (AB275045)

Western blot - Apoptosis pathway knockout cell lysates panel (AB275045)

Western blot - Apoptosis pathway knockout cell lysates panel (AB275045)

Western blot - Apoptosis pathway knockout cell lysates panel (AB275045)

Western blot - Apoptosis pathway knockout cell lysates panel (AB275045)

Western blot - Apoptosis pathway knockout cell lysates panel (AB275045)

Western blot - Apoptosis pathway knockout cell lysates panel (AB275045)

Western blot - Apoptosis pathway knockout cell lysates panel (AB275045)

Western blot - Apoptosis pathway knockout cell lysates panel (AB275045)

Western blot - Apoptosis pathway knockout cell lysates panel (AB275045)

Western blot - Apoptosis pathway knockout cell lysates panel (AB275045)

Western blot - Apoptosis pathway knockout cell lysates panel (AB275045)

Western blot - Apoptosis pathway knockout cell lysates panel (AB275045)

Western blot - Apoptosis pathway knockout cell lysates panel (AB275045)

Western blot - Apoptosis pathway knockout cell lysates panel (AB275045)

Key facts

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Reactivity data

Product details

What's included?

Properties and storage information

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Supplementary information

Biological function summary

Pathways

Product protocols

Product promise