Bag5 overexpression 293T lysate (whole cell)

Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The BAG5 protein also known as BCL2-associated athanogene 5 plays an important role as a co-chaperone in the regulation of protein folding and degradation. It has a molecular weight of approximately 48 kDa. BAG5 is expressed in various tissues but shows higher levels in neuronal tissues. The protein interacts with components of the cellular machinery to influence the fate of client proteins affecting their stability and function. Its structural regions facilitate binding to both target proteins and other chaperones modulating chaperone activity.

Biological function summary

BAG5 influences processes related to cell survival and apoptosis regulation. It is involved in forming complexes with heat shock proteins particularly Hsp70 and Hsp90 affecting their chaperone activity. This interaction impacts the protein's role in maintaining cellular proteostasis under stress conditions. BAG5 also plays a role in modulating the balance between pro-survival and pro-apoptotic signals by regulating Bcl-2 family members integrating signals that affect cell fate decisions.

Pathways

BAG5 participates in cellular stress response and apoptosis pathways. It functions within the endoplasmic reticulum-associated degradation (ERAD) pathway where it helps in the recognition and disposal of misfolded proteins. BAG5 has related interactions with other proteins like CHIP (C-terminus of Hsc70-interacting protein) and ubiquitin-protein ligases which are important in the ubiquitin-proteasome system. These interactions position BAG5 as an important player in managing protein quality control alongside regulating stress responses.

BAG5 relates to neurodegenerative diseases such as Parkinson's disease (PD) and Alzheimer's disease (AD). The protein interacts with Parkinson’s disease-associated protein alpha-synuclein influencing its aggregation and toxicity. In Alzheimer's disease BAG5 modulates the function of tau protein which aggregates abnormally in affected neurons. Regulation of these proteins by BAG5 impacts disease progression highlighting BAG5 as a potential therapeutic target.