E. coli Positive Control (Escherichia coli ) Whole Cell Lysate suitable for WB. View our extensive range of validated lysates from normal and diseased human, mouse and rat tissue.
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- Cell reports 27:955-970.e72019A Comprehensive Drosophila melanogaster Transcription Factor Interactome.Applications:Unspecified applicationReactive species:Unspecified reactive speciesLeila Shokri,Sachi Inukai,Antonina Hafner,Kathryn Weinand,Korneel Hens,Anastasia Vedenko,Stephen S Gisselbrecht,Riccardo Dainese,Johannes Bischof,Edy Furger,Jean-Daniel Feuz,Konrad Basler,Bart Deplancke,Martha L BulykPubMed 30995488
- PloS one 8:e816962013Improved coomassie blue dye-based fast staining protocol for proteins separated by SDS-PAGE.Applications:Unspecified applicationReactive species:Unspecified reactive speciesPavel Májek et. al.PubMed 24278455
- Nucleic acids research 39:6369-792011Gcn5 facilitates Pol II progression, rather than recruitment to nucleosome-depleted stress promoters, in Schizosaccharomyces pombe.
Key facts
- Form
- Liquid
Target data
Alternative names
Escherichia coli
Recommended products
E. coli Positive Control (Escherichia coli ) Whole Cell Lysate suitable for WB. View our extensive range of validated lysates from normal and diseased human, mouse and rat tissue.
Key facts
- Form
- Liquid
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- -20°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Notes
This E.coli lysate contains a 41 kd recombinant protein expressing 12 commonly used Tags which are fused to the N-terminus of the protein in the order of:
GST
T7
HSV
C-Myc
VSV-g
Glu-Glu
V5
E-tag
FLAG (DDDDK)
S-tag
HA
6x His
Excellent as a loading control for Western blotting. Lysate expresses 12 different epitope tags.
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7 product images
Western blot - E. coli Positive Control (Escherichia coli ) Whole Cell Lysate (ab5395)
Western blot - E. coli Positive Control (Escherichia coli ) Whole Cell Lysate (ab5395)
This blot was produced using a 4-12% Bis-tris gel under the MOPS buffer system. The gel was run at 200V for 50 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 3% milk before being incubated with HRP Anti-6X His tag® antibody [4D11] ab202004 overnight at 4°C. Antibody binding was visualised using ECL development solution ECL Substrate Kit (High Sensitivity) ab133406.
All lanes: Western blot - HRP Anti-6X His tag® antibody [4D11] (HRP Anti-6X His tag® antibody [4D11] ab202004) at 1/5000 dilution
All lanes: Western blot - E. coli Positive Control (Escherichia coli ) Whole Cell Lysate (ab5395) at 0.1 µg
Developed using the ECL technique.
Performed under reducing conditions.
Exposure time: 3min
Western blot - E. coli Positive Control (Escherichia coli ) Whole Cell Lysate (ab5395)
This image was produced using the same antibody clone but different formulation, Anti-Myc tag antibody [9E10] ab32.
Lysate from E. coli recombinantly expressing 11 commonly used tags including myc tag.
All lanes: Western blot - Anti-Myc tag antibody [9E10] (Anti-Myc tag antibody [9E10] ab32) at 1 µg/mL
All lanes: Western blot - E. coli Positive Control (Escherichia coli ) Whole Cell Lysate (ab5395) at 10 µg
SecondaryAll lanes: Goat polyclonal to Mouse IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 48 kDa, 49 kDa
Observed band size: 45 kDa
Exposure time: 1min
Western blot - E. coli Positive Control (Escherichia coli ) Whole Cell Lysate (ab5395)
This WB was performed using an E. coli lysate that expresses a 41kDa recombinant protein comprised of 12 commonly used epitope tags (T7, HSV, C-Myc, VSV-g, Glu-Glu, V5, E-tag, FLAG, S-tag, HA and 6xHis). This lysate is available as ab5395.
Western blot - E. coli Positive Control (Escherichia coli ) Whole Cell Lysate (ab5395)
All lanes: Western blot - Anti-Myc tag antibody [9E11] (Anti-Myc tag antibody [9E11] ab56) at 1 µg/mL
Lane 1: HEK293 cell lysate at 20 µg
Lane 2: Western blot - Recombinant Human c-Myc protein (Recombinant Human c-Myc protein ab84132) at 0.1 µg
Lane 3: Western blot - E. coli Positive Control (Escherichia coli ) Whole Cell Lysate (ab5395) at 0.1 µg
SecondaryAll lanes: Goat polyclonal to Mouse IgG H&L Pre-Adsorbed (HRP) at 1/5000 dilution
Predicted band size: 49 kDa
Western blot - E. coli Positive Control (Escherichia coli ) Whole Cell Lysate (ab5395)
Abcam recommends using 3% milk as the blocking agent.
All lanes: Western blot - HRP Anti-HA tag antibody (HRP Anti-HA tag antibody ab128131) at 1/5000 dilution
Lane 1: HEK-293 (Human) Whole Cell Lysate - expressing HA tag protein (ab20896) at 10 µg
Lane 2: Western blot - E. coli Positive Control (Escherichia coli ) Whole Cell Lysate (ab5395) at 0.1 µg
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 64 kDa
Observed band size: 34 kDa, 48 kDa, 55 kDa
Exposure time: 150s
Western blot - E. coli Positive Control (Escherichia coli ) Whole Cell Lysate (ab5395)
All lanes: Western blot - HRP Anti-Myc tag antibody [9E10] (HRP Anti-Myc tag antibody [9E10] ab62928) at 1/10000 dilution
All lanes: Western blot - E. coli Positive Control (Escherichia coli ) Whole Cell Lysate (ab5395) at 0.1 µg
Performed under reducing conditions.
Predicted band size: 49 kDa
Observed band size: 41 kDa
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