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E. coli Positive Control (Escherichia coli ) Whole Cell Lysate - expressing 6X His tag protein suitable for WB. View our extensive range of validated lysates from normal and diseased human, mouse and rat tissue.

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Images

Western blot - E. coli Positive Control (Escherichia coli ) Whole Cell Lysate - expressing 6X His tag protein (AB2431), expandable thumbnail
  • Western blot - E. coli Positive Control (Escherichia coli ) Whole Cell Lysate - expressing 6X His tag protein (AB2431), expandable thumbnail

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Key facts

Species or organism
Escherichia coli
Form
Lyophilized

Target data

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E. coli Positive Control (Escherichia coli ) Whole Cell Lysate - expressing 6X His tag protein suitable for WB. View our extensive range of validated lysates from normal and diseased human, mouse and rat tissue.

Key facts

Form
Lyophilized
Reconstitution
reconstitute with SDS-gel sample buffer at 250µL
Concentration
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Storage

Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle

Notes

This lysate can be used as a positive control for Western blots using lysates containing 6X-His tagged proteins.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

2 product images

  • Western blot - E. coli Positive Control (Escherichia coli ) Whole Cell Lysate - expressing 6X His tag protein (ab2431), expandable thumbnail

    Western blot - E. coli Positive Control (Escherichia coli ) Whole Cell Lysate - expressing 6X His tag protein (ab2431)

    All lanes: Rabbit anti-His-tag antibody

    Lane 1: Western blot - E. coli Positive Control (Escherichia coli ) Whole Cell Lysate - expressing 6X His tag protein (ab2431) at 2 µg

    Lane 2: Western blot - E. coli Positive Control (Escherichia coli ) Whole Cell Lysate - expressing 6X His tag protein (ab2431) at 5 µg

    Lane 3: Western blot - E. coli Positive Control (Escherichia coli ) Whole Cell Lysate - expressing 6X His tag protein (ab2431) at 10 µg

    Lane 4: Western blot - E. coli Positive Control (Escherichia coli ) Whole Cell Lysate - expressing 6X His tag protein (ab2431) at 15 µg

    Lane 5: Western blot - E. coli Positive Control (Escherichia coli ) Whole Cell Lysate - expressing 6X His tag protein (ab2431) at 20 µg

    Secondary

    All lanes: Goat anti-rabbit antibody (AP-conjugated)

  • Western blot - E. coli Positive Control (Escherichia coli ) Whole Cell Lysate - expressing 6X His tag protein (ab2431), expandable thumbnail

    Western blot - E. coli Positive Control (Escherichia coli ) Whole Cell Lysate - expressing 6X His tag protein (ab2431)

    All lanes: Western blot - Anti-6X His tag® antibody (Anti-6X His tag® antibody ab14923) at 0.6 µg/mL

    All lanes: Western blot - E. coli Positive Control (Escherichia coli ) Whole Cell Lysate - expressing 6X His tag protein (ab2431) at 10 µg

    Secondary

    All lanes: Alexa Fluor Goat polyclonal to Rabbit IgG at 1/10000 dilution

    Performed under reducing conditions.

    Observed band size: 17 kDa

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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