E. coli Positive Control (Escherichia coli ) Whole Cell Lysate - expressing 6X His tag protein suitable for WB. View our extensive range of validated lysates from normal and diseased human, mouse and rat tissue.
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- International journal of molecular sciences 25:2024Ripasudil as a Potential Therapeutic Agent in Treating Secondary Glaucoma in HTLV-1-Uveitis: An In Vitro Analysis.Applications:Unspecified applicationReactive species:Unspecified reactive speciesMingming Yang,Koju Kamoi,Yuan Zong,Jing Zhang,Yaru Zou,Kyoko Ohno-MatsuiPubMed 38542203
- Heliyon 9:e201852023Fibronectin promotes tumor progression through integrin αvβ3/PI3K/AKT/SOX2 signaling in non-small cell lung cancer.Applications:Unspecified applicationReactive species:Unspecified reactive speciesJin-Long Wu,Cheng-Feng Xu,Xu-Hui Yang,Ming-Song WangPubMed 37809806
- Nature communications 11:32562020NOTCH1 activation compensates BRCA1 deficiency and promotes triple-negative breast cancer formation.Applications:Unspecified applicationReactive species:Unspecified reactive speciesKai Miao et. al.PubMed 32591500
- Molecules (Basel, Switzerland) 24:2019Biological Effects of Shikonin in Human Gingival Fibroblasts via ERK 1/2 Signaling Pathway.Applications:Unspecified applicationReactive species:Unspecified reactive speciesKazutaka Imai et. al.PubMed 31574951
- Molecular & cellular proteomics : MCP 11:478-912012Enhanced peptide identification by electron transfer dissociation using an improved Mascot Percolator.Applications:Unspecified applicationReactive species:Unspecified reactive speciesJames C Wright,Mark O Collins,Lu Yu,Lukas Käll,Markus Brosch,Jyoti S ChoudharyPubMed 22493177
Key facts
- Species or organism
- Escherichia coli
- Form
- Lyophilized
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E. coli Positive Control (Escherichia coli ) Whole Cell Lysate - expressing 6X His tag protein suitable for WB. View our extensive range of validated lysates from normal and diseased human, mouse and rat tissue.
Key facts
- Species or organism
- Escherichia coli
- Form
- Lyophilized
- Reconstitution
- reconstitute with SDS-gel sample buffer at 250µL
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage duration
- 1-2 weeks
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Notes
This lysate can be used as a positive control for Western blots using lysates containing 6X-His tagged proteins.
Product promise
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In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
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2 product images
Western blot - E. coli Positive Control (Escherichia coli ) Whole Cell Lysate - expressing 6X His tag protein (ab2431)
All lanes: Rabbit anti-His-tag antibody
Lane 1: Western blot - E. coli Positive Control (Escherichia coli ) Whole Cell Lysate - expressing 6X His tag protein (ab2431) at 2 µg
Lane 2: Western blot - E. coli Positive Control (Escherichia coli ) Whole Cell Lysate - expressing 6X His tag protein (ab2431) at 5 µg
Lane 3: Western blot - E. coli Positive Control (Escherichia coli ) Whole Cell Lysate - expressing 6X His tag protein (ab2431) at 10 µg
Lane 4: Western blot - E. coli Positive Control (Escherichia coli ) Whole Cell Lysate - expressing 6X His tag protein (ab2431) at 15 µg
Lane 5: Western blot - E. coli Positive Control (Escherichia coli ) Whole Cell Lysate - expressing 6X His tag protein (ab2431) at 20 µg
SecondaryAll lanes: Goat anti-rabbit antibody (AP-conjugated)
Western blot - E. coli Positive Control (Escherichia coli ) Whole Cell Lysate - expressing 6X His tag protein (ab2431)
All lanes: Western blot - Anti-6X His tag® antibody (Anti-6X His tag® antibody ab14923) at 0.6 µg/mL
All lanes: Western blot - E. coli Positive Control (Escherichia coli ) Whole Cell Lysate - expressing 6X His tag protein (ab2431) at 10 µg
SecondaryAll lanes: Alexa Fluor Goat polyclonal to Rabbit IgG at 1/10000 dilution
Performed under reducing conditions.
Observed band size: 17 kDa
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