E. coli Positive Control (Escherichia coli ) Whole Cell Lysate - expressing 6X His tag protein suitable for WB. View our extensive range of validated lysates from normal and diseased human, mouse and rat tissue.
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E. coli Positive Control (Escherichia coli ) Whole Cell Lysate - expressing 6X His tag protein suitable for WB. View our extensive range of validated lysates from normal and diseased human, mouse and rat tissue.
This lysate can be used as a positive control for Western blots using lysates containing 6X-His tagged proteins.
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All lanes: Rabbit anti-His-tag antibody
Lane 1: Western blot - E. coli Positive Control (Escherichia coli ) Whole Cell Lysate - expressing 6X His tag protein (ab2431) at 2 µg
Lane 2: Western blot - E. coli Positive Control (Escherichia coli ) Whole Cell Lysate - expressing 6X His tag protein (ab2431) at 5 µg
Lane 3: Western blot - E. coli Positive Control (Escherichia coli ) Whole Cell Lysate - expressing 6X His tag protein (ab2431) at 10 µg
Lane 4: Western blot - E. coli Positive Control (Escherichia coli ) Whole Cell Lysate - expressing 6X His tag protein (ab2431) at 15 µg
Lane 5: Western blot - E. coli Positive Control (Escherichia coli ) Whole Cell Lysate - expressing 6X His tag protein (ab2431) at 20 µg
All lanes: Goat anti-rabbit antibody (AP-conjugated)
All lanes: Western blot - Anti-6X His tag® antibody (Anti-6X His tag® antibody ab14923) at 0.6 µg/mL
All lanes: Western blot - E. coli Positive Control (Escherichia coli ) Whole Cell Lysate - expressing 6X His tag protein (ab2431) at 10 µg
All lanes: Alexa Fluor Goat polyclonal to Rabbit IgG at 1/10000 dilution
Performed under reducing conditions.
Observed band size: 17 kDa
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