E. coli Positive Control (Escherichia coli ) Whole Cell Lysate - expressing 6X His tag protein

• WB

Project

Western blot - E. coli Positive Control (Escherichia coli ) Whole Cell Lysate - expressing 6X His tag protein (AB2431)

All lanes:

Western blot - Anti-6X His tag® antibody (<a href='/en-us/products/primary-antibodies/6x-his-tag-antibody-ab14923'>ab14923</a>) at 0.6 µg/mL

All lanes:

Western blot - E. coli Positive Control (Escherichia coli ) Whole Cell Lysate - expressing 6X His tag protein (ab2431) at 10 µg

Secondary

All lanes:

Alexa Fluor Goat polyclonal to Rabbit IgG at 1/10000 dilution

Observed band size: 17 kDa

false

• WB

Supplier Data

Western blot - E. coli Positive Control (Escherichia coli ) Whole Cell Lysate - expressing 6X His tag protein (AB2431)

All lanes:

Rabbit anti-His-tag antibody

Lane 1:

Western blot - E. coli Positive Control (Escherichia coli ) Whole Cell Lysate - expressing 6X His tag protein (ab2431) at 2 µg

Lane 2:

Western blot - E. coli Positive Control (Escherichia coli ) Whole Cell Lysate - expressing 6X His tag protein (ab2431) at 5 µg

Lane 3:

Western blot - E. coli Positive Control (Escherichia coli ) Whole Cell Lysate - expressing 6X His tag protein (ab2431) at 10 µg

Lane 4:

Western blot - E. coli Positive Control (Escherichia coli ) Whole Cell Lysate - expressing 6X His tag protein (ab2431) at 15 µg

Lane 5:

Western blot - E. coli Positive Control (Escherichia coli ) Whole Cell Lysate - expressing 6X His tag protein (ab2431) at 20 µg

Secondary

All lanes:

Goat anti-rabbit antibody (AP-conjugated)

false