eIF5A2 overexpression 293T lysate (whole cell)

Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

EIF5A2 also known as eukaryotic translation initiation factor 5A isoform 2 is a small protein about 17 kDa in mass. It is involved in translation elongation and facilitates the assembly of ribosomes on mRNA during protein synthesis. The protein is ubiquitously expressed in many tissues with high levels of expression in cancer cells compared to normal tissues. eIF5A2 possesses a unique post-translational modification hypusination which is important for its function.

Biological function summary

EIF5A2 promotes the synthesis of proteins essential for cell proliferation and survival. It does this by binding to mRNAs and enhancing the translation of specific genes that are often implicated in cancer development. eIF5A2 is not part of a larger protein complex but works in conjunction with ribosomal units during the translation process. Its hypusinated form is responsible for the specific and efficient translation process that influences cellular growth and apoptosis.

Pathways

EIF5A2 functions as a significant player in the pathways related to translation and cell cycle regulation. Specifically it interacts within the mTOR and Wnt signaling pathways. These pathways are associated with cell growth and cancer involving proteins like mTOR and β-catenin respectively which are vital for regulating processes such as cell growth proliferation and differentiation.

EIF5A2 has strong relevance to cancer particularly in its overexpression linked to tumor progression and metastasis in various cancers such as colorectal and ovarian cancer. In these cancers the expression of eIF5A2 often correlates with poor prognosis. It is associated with proteins like matrix metalloproteinases (MMPs) which play a role in cancer cell invasion and metastasis. Moreover abnormalities in eIF5A2 expression or activity might also relate to neurodegenerative disorders providing a potential target for treatment strategies in these diseases.