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AB94133

EpCAM overexpression 293T lysate (whole cell)

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EpCAM overexpression 293T lysate (whole cell) suitable for WB. View our extensive range of validated lysates from normal and diseased human, mouse and rat tissue.

View Alternative Names

17 1A, 323/A3, AUA1, Adenocarcinoma-associated antigen, Antigen identified by monoclonal antibody AUA1, CD326, CD326 antigen, CO 17A, CO17 1A, Cell surface glycoprotein Trop-1, Cell surface glycoprotein Trop-2, DIAR5, EGP 2, EGP314, EGP40, EPCAM_HUMAN, ESA, EpCAM1, Epithelial Cell Adhesion Molecule Intracellular Domain (EpCAM-ICD), Epithelial cell adhesion molecule, Epithelial cell surface antigen, Epithelial cellular adhesion molecule, Epithelial glycoprotein, Epithelial glycoprotein 1, Epithelial glycoprotein 314, GA733 1, GA733-2, GP4, HNPCC8, Human epithelial glycoprotein 2, KS 1/4 antigen, KS1/4, KSA, Ly74, Lymphocyte antigen 74, M1S 1, M1S2, M4S1, MIC18, MK 1, Major gastrointestinal tumor-associated protein GA733-2, Membrane component chromosome 4 surface marker (35kD glycoprotein), Membrane component, chromosome 4, surface marker, Membrane component, chromosome 4, surface marker 1, Protein 289A, TACD1, TACSTD1, TROP1, Tumor associated calcium signal transducer 2 precursor, Tumor-associated calcium signal transducer 1, gastrointestinal tumor-associated antigen 2, 35-KD glycoprotein, hEGP 2, hEGP314, mEGP314

2 Images
Western blot - EpCAM overexpression 293T lysate (whole cell) (AB94133)
  • WB

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Western blot - EpCAM overexpression 293T lysate (whole cell) (AB94133)

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SDS-PAGE - EpCAM overexpression 293T lysate (whole cell) (AB94133)
  • SDS-PAGE

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SDS-PAGE - EpCAM overexpression 293T lysate (whole cell) (AB94133)

ab94133 at 15µg/lane on an SDS-PAGE gel.

Key facts

Species or organism

Human

Form

Liquid

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "WB": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Product details

ab94133 is a 293T cell transfected lysate in which Human EpCAM has been transiently over-expressed using a pCMV-EpCAM plasmid. The lysate is provided in 1X Sample Buffer.
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Properties and storage information

Shipped at conditions
Dry Ice
Appropriate short-term storage conditions
-20°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

EpCAM also known as epithelial cell adhesion molecule is a transmembrane glycoprotein with a molecular weight of around 40 kDa. Alternative names include CD326 and TACSTD1. This protein is expressed on the surface of most epithelial cells and plays a role in cell adhesion. It is involved in maintaining the integrity of epithelial tissues by mediating homotypic cell-cell interactions which are important for the structure and function of tissues.
Biological function summary

EpCAM facilitates signaling pathways that impact cellular processes like proliferation and differentiation. It is not a part of a larger protein complex but interacts directly with other cellular machinery to transmit intracellular signals. EpCAM influences stem cell behavior and is important in tissue homeostasis assisting in both normal regenerative processes and wound healing.

Pathways

EpCAM participates in regulatory mechanisms involving the Wnt/β-catenin signaling pathway which affects gene expression and cell fate decisions. Through its action EpCAM modulates components like E-cadherin which is important for cell adhesion and the maintenance of the epithelial layer. It indirectly influences pathways relating to MYC a transcription factor that regulates cell cycle progression and apoptosis.

EpCAM is associated with various types of cancer including colorectal and breast cancer. Its overexpression often correlates with aggressive cancer phenotypes and poor prognosis. Besides cancers EpCAM mutations can lead to congenital tufting enteropathy a rare condition affecting intestinal epithelial structure and function. Aberrant EpCAM expression affects β-catenin levels impacting cellular pathways linked to tumor progression and epithelial integrity.
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Cell culture

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Product protocols

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Complementary Products

Primary Antibodies

AB237385

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Assay Kits

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Proteins & Peptides

AB151338

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