GPAM overexpression 293T lysate (whole cell)

Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

GPAM also known as glycerol-3-phosphate acyltransferase mitochondrial is an enzyme that plays an important role in lipid biosynthesis. This enzyme which consists of approximately 828 amino acids has a mass of around 92 kilodaltons (kDa). GPAM is mainly found in the mitochondria of cells and exhibits high expression levels in tissues such as adipose tissue liver and muscle. Its function involves the initial steps in the synthesis of glycerolipids by catalyzing the esterification of glycerol-3-phosphate with long-chain fatty acids.

Biological function summary

The enzyme influences important lipid metabolic processes across different tissues. GPAM initiates the triacylglycerol biosynthesis pathway by producing lysophosphatidic acid which is a precursor to phosphatidic acid. It does not form a direct complex with other proteins but it does interact with lipid metabolic processes and affects the overall homeostasis of lipids within the cell. It impacts energy storage in adipocytes and has a role in liver metabolic regulation.

Pathways

GPAM participates in the glycerolipid and glycerophospholipid metabolism pathways. It functions at the intersection of these pathways to regulate lipid accumulation and usage in cells especially in adipose tissue and liver. GPAM's activity connects with other enzymes such as diacylglycerol O-acyltransferase (DGAT) in the process of triacylglycerol synthesis and overall lipid metabolism.

GPAM has been linked to obesity and non-alcoholic fatty liver disease (NAFLD). Elevated enzyme activity can lead to increased lipid storage contributing to obesity while dysregulation can play a role in the development of fatty liver conditions. GPAM interacts with proteins like adiponectin where altered pathways can influence metabolic syndromes and associated complications.