Hela-DFO treated (0.5mM, 24h) Nuclear Lysate

4 Images

• WB

Unknown

Western blot - Hela-DFO treated (0.5mM, 24h) Nuclear Lysate (AB180880)

Abcam recommends using 5% milk as the blocking agent, decreasing to 2% milk during primary and secondary incubation. Abcam welcomes customer feedback and would appreciate any comments regarding this product and the data presented above.

All lanes:

Western blot - Anti-HIF-1 alpha antibody [EP1215Y] (<a href='/en-us/products/primary-antibodies/hif-1-alpha-antibody-ep1215y-ab51608'>ab51608</a>) at 1/2000 dilution

Lane 1:

Western blot - HeLa nuclear extract lysate (<a href='/en-us/products/cell-lysates/hela-nuclear-extract-lysate-ab150036'>ab150036</a>) at 40 µg

Lane 2:

Western blot - Hela-DFO treated (0.5mM, 24h) Nuclear Lysate (ab180880) at 40 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/10000 dilution

Predicted band size: 92 kDa

Observed band size: 110 kDa

true

Exposure time: 8min

• WB

Lab

Western blot - Hela-DFO treated (0.5mM, 24h) Nuclear Lysate (AB180880)

We recommend using 5% milk in TBST as the blocking agent, decreasing to 2% milk in TBST during primary and secondary antibody incubation.

Blots were developed with Goat Anti-Mouse IgG H&L (HRP) preadsorbed (ab97040) secondary antibody

All lanes:

Western blot - Anti-HIF-1 alpha antibody [H1alpha67] (<a href='/en-us/products/primary-antibodies/hif-1-alpha-antibody-h1alpha67-ab1'>ab1</a>) at 5 µg/mL

Lane 1:

Western blot - HeLa nuclear extract lysate (<a href='/en-us/products/cell-lysates/hela-nuclear-extract-lysate-ab150036'>ab150036</a>) at 40 µg

Lane 2:

Western blot - Hela-DFO treated (0.5mM, 24h) Nuclear Lysate (ab180880) at 40 µg

Lane 3:

HeLa nuclear control at 40 µg

Lane 4:

HeLa nuclear DFO treated at 40 µg

Lane 5:

Western blot - Recombinant Human HIF-1 alpha protein (<a href='/en-us/products/proteins-peptides/recombinant-human-hif-1-alpha-protein-ab154478'>ab154478</a>) at 0.001 µg

Secondary

All lanes:

Western blot - Goat Anti-Mouse IgG H&L (HRP) preadsorbed (<a href='/en-us/products/secondary-antibodies/goat-mouse-igg-h-l-hrp-preadsorbed-ab97040'>ab97040</a>) at 1/10000 dilution

Predicted band size: 92 kDa

false

Exposure time: 20min

• WB

Unknown

Western blot - Hela-DFO treated (0.5mM, 24h) Nuclear Lysate (AB180880)

false

• WB

Unknown

Western blot - Hela-DFO treated (0.5mM, 24h) Nuclear Lysate (AB180880)

false