HeLa whole cell lysate

• WB

Lab

Western blot - HeLa whole cell lysate (AB150035)

All lanes:

Western blot - HRP Anti-Cyclin E1 antibody [EP435E] (<a href='/en-us/products/primary-antibodies/hrp-cyclin-e1-antibody-ep435e-ab194070'>ab194070</a>) at 1/5000 dilution

All lanes:

Western blot - HeLa whole cell lysate (ab150035) at 20 µg

false

• WB

Lab

Western blot - HeLa whole cell lysate (AB150035)

This blot was produced using a 4-12% Bis-tris gel under the MES buffer system. The gel was run at 200V for 35 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 2% Bovine Serum Albumin before being incubated with ab209023 overnight at 4°C. Antibody binding was visualised using ECL development solution ab133406.

All lanes:

Western blot - HRP Anti-Histone H3 antibody [EPR16987] - Nuclear Loading Control (<a href='/en-us/products/primary-antibodies/hrp-histone-h3-antibody-epr16987-nuclear-loading-control-ab209023'>ab209023</a>) at 1/5000 dilution

Lane 1:

Western blot - HeLa whole cell lysate (ab150035) at 10 µg

Lane 2:

NIH 3T3 (Mouse embryonic fibroblast cell line) Whole Cell Lysate at 10 µg

Lane 3:

Drosophila embryo nuclear extract (from melanogaster embryos 0-12hr) at 10 µg

Predicted band size: 15 kDa

Observed band size: 17 kDa

true

Exposure time: 2min

• WB

Lab

Western blot - HeLa whole cell lysate (AB150035)

This blot was produced using a 4-12% Bis-tris gel under the MOPS buffer system. The gel was run at 200V for 50 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 3% milk before being incubated with ab205819 overnight at 4°C. Antibody binding was visualised using ECL development solution ab133406.

All lanes:

Western blot - HRP Anti-ERK2 antibody [E460] (<a href='/en-us/products/primary-antibodies/hrp-erk2-antibody-e460-ab205819'>ab205819</a>) at 1/5000 dilution

Lane 1:

Western blot - HeLa whole cell lysate (ab150035) at 10 µg

Lane 2:

46C (Mouse neural progenitor, selected for Sox1 expression cell line) Whole Cell Lysate at 10 µg

Lane 3:

PC12 (Rat adrenal pheochromocytoma cell line) Whole Cell Lysate at 10 µg

Lane 4:

Recombinant Human ERK1 protein (<a href='/en-us/products/unavailable/recombinant-human-erk1-protein-ab43623-ab43623'>ab43623</a>) (<a href='/en-us/products/unavailable/recombinant-human-erk1-protein-ab43623-ab43623'>ab43623</a>) at 0.1 µg

Lane 5:

Western blot - Recombinant Human ERK2 protein (<a href='/en-us/products/proteins-peptides/recombinant-human-erk2-protein-ab43625'>ab43625</a>) at 0.1 µg

Lane 6:

Wild-type HAP1 cell lysate at 20 µg

Lane 7:

ERK2 knockout HAP1 cell lysate at 20 µg

Predicted band size: 41 kDa

Observed band size: 41 kDa

true

Exposure time: 12min

• WB

Lab

Western blot - HeLa whole cell lysate (AB150035)

This blot was produced using a 4-12% Bis-tris gel under the MES buffer system. The gel was run at 200V for 35 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 3% milk before being incubated with ab209046 overnight at 4°C. Antibody binding was visualised using ECL development solution ab133406.

All lanes:

Western blot - HRP Anti-RAB8A antibody [EPR14873] - C-terminal (<a href='/en-us/products/primary-antibodies/hrp-rab8a-antibody-epr14873-c-terminal-ab209046'>ab209046</a>) at 1/5000 dilution

Lane 1:

Western blot - HeLa whole cell lysate (ab150035) at 10 µg

Lane 2:

HCT 116 (Human Colorectal Carcinoma) Whole Cell Lysate at 10 µg

Lane 3:

PC12 (Rat adrenal pheochromocytoma cell line) Whole Cell Lysate at 10 µg

Lane 4:

Wild-type HAP1 cell lysate at 20 µg

Lane 5:

RAB8A knockout HAP1 cell lysate at 20 µg

Predicted band size: 24 kDa

Observed band size: 24 kDa

true

Exposure time: 20min

• WB

Lab

Western blot - HeLa whole cell lysate (AB150035)

This blot was produced using a 4-12% Bis-tris gel under the MOPS buffer system. The gel was run at 200V for 50 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 2% Bovine Serum Albumin before being incubated with ab205790 overnight at 4°C. Antibody binding was visualised using ECL development solution ab133406.

All lanes:

Western blot - HRP Anti-beta Tubulin antibody [EPR16774] - Loading Control (<a href='/en-us/products/primary-antibodies/hrp-beta-tubulin-antibody-epr16774-loading-control-ab205790'>ab205790</a>) at 1/5000 dilution

Lane 1:

Western blot - HeLa whole cell lysate (ab150035) at 10 µg

Lane 2:

NIH 3T3 (Mouse embryonic fibroblast cell line) Whole Cell Lysate at 10 µg

Lane 3:

PC12 (Rat adrenal pheochromocytoma cell line) Whole Cell Lysate at 10 µg

Lane 4:

Brain (Mouse) Tissue Lysate at 10 µg

Lane 5:

Brain (Rat) Tissue Lysate at 10 µg

Predicted band size: 49 kDa

Observed band size: 52 kDa

true

Exposure time: 5s

• WB

Lab

Western blot - HeLa whole cell lysate (AB150035)

This blot was produced using a 4-12% Bis-tris gel under the MOPS buffer system. The gel was run at 200V for 50 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 2% Bovine Serum Albumin before being incubated with ab199026 overnight at 4°C. Antibody binding was visualised using ECL development solution ab133406.

All lanes:

Western blot - HRP Anti-DDB1 antibody [EPR6089] (<a href='/en-us/products/primary-antibodies/hrp-ddb1-antibody-epr6089-ab199026'>ab199026</a>) at 1/5000 dilution

Lane 1:

HepG2 (Human hepatocellular liver carcinoma cell line) Whole Cell Lysate at 10 µg

Lane 3:

NIH 3T3 (Mouse embryonic fibroblast cell line) Whole Cell Lysate at 10 µg

Lane 4:

Human placenta tissue lysate - total protein (<a href='/en-us/products/unavailable/human-placenta-tissue-lysate-total-protein-ab29745'>ab29745</a>) at 10 µg

Predicted band size: 127 kDa

Observed band size: 127 kDa

true

Exposure time: 20s

• WB

Lab

Western blot - HeLa whole cell lysate (AB150035)

This blot was produced using a 4-12% Bis-tris gel under the MOPS buffer system. The gel was run at 200V for 50 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 3% milk before being incubated with ab209317 overnight at 4°C. Antibody binding was visualised using ECL development solution ab133406.

All lanes:

Western blot - HRP Anti-ASH2L antibody [EPR13106] (<a href='/en-us/products/primary-antibodies/hrp-ash2l-antibody-epr13106-ab209317'>ab209317</a>) at 1/3000 dilution

Lane 1:

HEK293 (Human embryonic kidney cell line) Whole Cell Lysate at 10 µg

Lane 2:

Western blot - HeLa whole cell lysate (ab150035) at 10 µg

Lane 3:

C6 (Rat glioma cell line) Whole Cell Lysate at 10 µg

Lane 4:

PC12 (Rat adrenal pheochromocytoma cell line) Whole Cell Lysate at 10 µg

Predicted band size: 69 kDa

Observed band size: 80 kDa

true

Exposure time: 20min

• WB

Lab

Western blot - HeLa whole cell lysate (AB150035)

This blot was produced using a 4-12% Bis-tris gel under the MOPS buffer system. The gel was run at 200V for 50 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 3% milk before being incubated with ab193891 overnight at 4°C. Antibody binding was visualised using ECL development solution ab133406.

All lanes:

Western blot - HRP Anti-STAT1 alpha antibody [EPYR2154] (<a href='/en-us/products/primary-antibodies/hrp-stat1-alpha-antibody-epyr2154-ab193891'>ab193891</a>) at 1/5000 dilution

Lane 1:

Western blot - HeLa whole cell lysate (ab150035) at 10 µg

Lane 2:

HEK293 (Human embryonic kidney cell line) Whole Cell Lysate at 10 µg

Lane 3:

NIH 3T3 (Mouse embryonic fibroblast cell line) Whole Cell Lysate at 20 µg

Lane 4:

A431 (Human epithelial carcinoma cell line) Whole Cell Lysate at 20 µg

Predicted band size: 87 kDa

Observed band size: 91 kDa

true

Exposure time: 2min

• WB

Lab

Western blot - HeLa whole cell lysate (AB150035)

This blot was produced using a 4-12% Bis-tris gel under the MOPS buffer system. The gel was run at 200V for 50 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 2% Bovine Serum Albumin before being incubated with ab211372 overnight at 4°C. Antibody binding was visualised using ECL development solution ab133406.

All lanes:

Western blot - HRP Anti-PPP1CA + PPP1CB antibody [EP1511Y] (<a href='/en-us/products/primary-antibodies/hrp-ppp1ca-ppp1cb-antibody-ep1511y-ab211372'>ab211372</a>) at 1/5000 dilution

Lane 1:

Brain (Mouse) Tissue Lysate at 10 µg

Lane 2:

Brain (Rat) Tissue Lysate at 10 µg

Lane 3:

Western blot - HeLa whole cell lysate (ab150035) at 10 µg

Predicted band size: 37 kDa

Observed band size: 36 kDa

true

Exposure time: 3min

• WB

Lab

Western blot - HeLa whole cell lysate (AB150035)

This blot was produced using a 4-12% Bis-tris gel under the MOPS buffer system. The gel was run at 200V for 50 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 2% Bovine Serum Albumin before being incubated with ab196602 overnight at 4°C. Antibody binding was visualised using ECL development solution ab133406.

All lanes:

Western blot - HRP Anti-Vimentin antibody [V9] - Cytoskeleton Marker (<a href='/en-us/products/primary-antibodies/hrp-vimentin-antibody-v9-cytoskeleton-marker-ab196602'>ab196602</a>) at 1/5000 dilution

Lane 1:

Western blot - HeLa whole cell lysate (ab150035) at 10 µg

Lane 2:

MOLT4 (Human acute lymphoblastic leukemia cell line) Whole Cell Lysate at 10 µg

Predicted band size: 53 kDa

Observed band size: 57 kDa

true

Exposure time: 8s

• WB

Lab

Western blot - HeLa whole cell lysate (AB150035)

This blot was produced using a 4-12% Bis-tris gel under the MOPS buffer system. The gel was run at 200V for 50 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 3% milk before being incubated with ab196715 overnight at 4°C. Antibody binding was visualised using ECL development solution ab133406.

All lanes:

Western blot - HRP Anti-Coilin antibody [IH10] (<a href='/en-us/products/primary-antibodies/hrp-coilin-antibody-ih10-ab196715'>ab196715</a>) at 1/2000 dilution

Lane 1:

Western blot - HeLa whole cell lysate (ab150035) at 10 µg

Lane 2:

MCF7 (Human breast adenocarcinoma cell line) Whole Cell Lysate at 10 µg

Lane 3:

SHSY-5Y (Human neuroblastoma cell line) Whole Cell Lysate at 10 µg

Predicted band size: 62 kDa

Observed band size: 75 kDa

true

Exposure time: 4min

• WB

Lab

Western blot - HeLa whole cell lysate (AB150035)

This blot was produced using a 4-12% Bis-tris gel under the MOPS buffer system. The gel was run at 200V for 50 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 2% Bovine Serum Albumin before being incubated with ab197905 overnight at 4°C. Antibody binding was visualised using ECL development solution ab133406.

All lanes:

Western blot - HRP Anti-ATPB antibody [3D5] - Mitochondrial Marker (<a href='/en-us/products/primary-antibodies/hrp-atpb-antibody-3d5-mitochondrial-marker-ab197905'>ab197905</a>) at 1/5000 dilution

Lane 1:

Human heart tissue lysate - mitochondrial extract (<a href='/en-us/products/unavailable/human-heart-tissue-lysate-mitochondrial-extract-ab110337'>ab110337</a>) at 5 µg

Lane 2:

Western blot - HeLa whole cell lysate (ab150035) at 10 µg

Lane 3:

HepG2 (Human hepatocellular liver carcinoma cell line) Whole Cell Lysate at 10 µg

Predicted band size: 56 kDa

Observed band size: 52 kDa

true

Exposure time: 2s

• WB

Lab

Western blot - HeLa whole cell lysate (AB150035)

This blot was produced using a 4-12% Bis-tris gel under the MOPS buffer system. The gel was run at 200V for 50 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 3% milk before being incubated with ab195854 overnight at 4°C. Antibody binding was visualised using ECL development solution ab133406.

All lanes:

Western blot - HRP Anti-NFkB p105 / p50 antibody [E381] (<a href='/en-us/products/primary-antibodies/hrp-nfkb-p105-p50-antibody-e381-ab195854'>ab195854</a>) at 1/2000 dilution

All lanes:

Western blot - HeLa whole cell lysate (ab150035) at 10 µg

Predicted band size: 105 kDa

Observed band size: 105 kDa,50 kDa

true

Exposure time: 20min

• WB

Lab

Western blot - HeLa whole cell lysate (AB150035)

This blot was produced using a 4-12% Bis-tris gel under the MOPS buffer system. The gel was run at 200V for 50 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 3% milk before being incubated with ab194720 overnight at 4°C. Antibody binding was visualised using ECL development solution ab133406.

All lanes:

Western blot - HRP Anti-SQSTM1 / p62 antibody [EPR4844] - Autophagosome Marker (<a href='/en-us/products/primary-antibodies/hrp-sqstm1-p62-antibody-epr4844-autophagosome-marker-ab194720'>ab194720</a>) at 1/1000 dilution

Lane 1:

MCF7 (Human breast adenocarcinoma cell line) Whole Cell Lysate at 10 µg

Lane 2:

Western blot - HeLa whole cell lysate (ab150035) at 10 µg

Predicted band size: 47 kDa

Observed band size: 62 kDa

true

Exposure time: 20s

• WB

Lab

Western blot - HeLa whole cell lysate (AB150035)

This blot was produced using a 4-12% Bis-tris gel under the MOPS buffer system. The gel was run at 200V for 50 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 3% milk before being incubated with ab202914 overnight at 4°C. Antibody binding was visualised using ECL development solution ab133406.

All lanes:

Western blot - HRP Anti-delta 1 Catenin/CAS antibody [YE372] (<a href='/en-us/products/primary-antibodies/hrp-delta-1-catenin-cas-antibody-ye372-ab202914'>ab202914</a>) at 1/5000 dilution

All lanes:

Western blot - HeLa whole cell lysate (ab150035) at 10 µg

Predicted band size: 108 kDa

Observed band size: 120 kDa

true

Exposure time: 1min

• WB

Lab

Western blot - HeLa whole cell lysate (AB150035)

This blot was produced using a 4-12% Bis-tris gel under the MES buffer system. The gel was run at 200V for 35 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 2% Bovine Serum Albumin before being incubated with ab194079 overnight at 4°C. Antibody binding was visualised using ECL development solution ab133406.

All lanes:

Western blot - HRP Anti-Hsp27 antibody [EPR5477] (<a href='/en-us/products/primary-antibodies/hrp-hsp27-antibody-epr5477-ab194079'>ab194079</a>) at 1/5000 dilution

All lanes:

Western blot - HeLa whole cell lysate (ab150035) at 10 µg

Predicted band size: 23 kDa

Observed band size: 27 kDa

true

Exposure time: 20s

• WB

Lab

Western blot - HeLa whole cell lysate (AB150035)

This blot was produced using a 3-8% Tris Acetate gel under the TA buffer system. The gel was run at 150V for 60 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 3% milk before being incubated with ab193602 overnight at 4°C. Antibody binding was visualised using ECL development solution ab133406.

All lanes:

Western blot - HRP Anti-EGFR antibody [EP38Y] (<a href='/en-us/products/primary-antibodies/hrp-egfr-antibody-ep38y-ab193602'>ab193602</a>) at 1/5000 dilution

All lanes:

Western blot - HeLa whole cell lysate (ab150035) at 10 µg

Predicted band size: 134 kDa

Observed band size: 175 kDa

true

Exposure time: 3min

• WB

Lab

Western blot - HeLa whole cell lysate (AB150035)

This blot was produced using a 4-12% Bis-tris gel under the MOPS buffer system. The gel was run at 200V for 50 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 3% milk before being incubated with ab195198 overnight at 4°C. Antibody binding was visualised using ECL development solution ab133406.

All lanes:

Western blot - HRP Anti-Calnexin antibody [EPR3633(2)] - ER Membrane Marker (<a href='/en-us/products/primary-antibodies/hrp-calnexin-antibody-epr36332-er-membrane-marker-ab195198'>ab195198</a>) at 1/5000 dilution

Lane 1:

HepG2 (Human hepatocellular liver carcinoma cell line) Whole Cell Lysate at 10 µg

Lane 2:

A431 (Human epithelial carcinoma cell line) Whole Cell Lysate at 10 µg

Lane 3:

Western blot - HeLa whole cell lysate (ab150035) at 10 µg

Predicted band size: 68 kDa

Observed band size: 82 kDa

true

Exposure time: 20min

• WB

Lab

Western blot - HeLa whole cell lysate (AB150035)

This blot was produced using a 4-12% Bis-tris gel under the MOPS buffer system. The gel was run at 200V for 50 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 3% milk before being incubated with ab196980 overnight at 4°C. Antibody binding was visualised using ECL development solution ab133406.

All lanes:

Western blot - HRP Anti-Fibrillarin antibody+Fibrillarin-like protein 1 [EPR10823(B)] - Nucleolar Marker (<a href='/en-us/products/primary-antibodies/hrp-fibrillarin-antibodyfibrillarin-like-protein-1-epr10823b-nucleolar-marker-ab196980'>ab196980</a>) at 1/5000 dilution

Lane 1:

HEK-293 (Human) Whole Cell Lysate (ab52256) at 10 µg

Lane 2:

Western blot - HeLa whole cell lysate (ab150035) at 10 µg

Lane 3:

HepG2 (Human hepatocellular liver carcinoma cell line) Whole Cell Lysate at 10 µg

Lane 4:

MOLT4 (Human acute lymphoblastic leukemia cell line) Whole Cell Lysate at 10 µg

Predicted band size: 33 kDa

Observed band size: 33 kDa

true

Exposure time: 8min

• WB

Lab

Western blot - HeLa whole cell lysate (AB150035)

This blot was produced using a 4-12% Bis-tris gel under the MOPS buffer system. The gel was run at 200V for 50 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 3% milk before being incubated with ab208366 overnight at 4°C. Antibody binding was visualised using ECL development solution ab133406.

All lanes:

Western blot - HRP Anti-Lactate Dehydrogenase B/LDH-B antibody [EP1565Y] (<a href='/en-us/products/primary-antibodies/hrp-lactate-dehydrogenase-b-ldh-b-antibody-ep1565y-ab208366'>ab208366</a>) at 1/5000 dilution

All lanes:

Western blot - HeLa whole cell lysate (ab150035) at 10 µg

Predicted band size: 37 kDa

Observed band size: 35 kDa

true

Exposure time: 30s

• WB

Lab

Western blot - HeLa whole cell lysate (AB150035)

This blot was produced using a 4-12% Bis-tris gel under the MOPS buffer system. The gel was run at 200V for 50 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 3% milk before being incubated with ab193598 overnight at 4°C. Antibody binding was visualised using ECL development solution ab133406.

All lanes:

Western blot - HRP Anti-Cyclin A2 antibody [E399] (<a href='/en-us/products/primary-antibodies/hrp-cyclin-a2-antibody-e399-ab193598'>ab193598</a>) at 1/1000 dilution

All lanes:

Western blot - HeLa whole cell lysate (ab150035) at 10 µg

Predicted band size: 49 kDa

Observed band size: 52 kDa

true

Exposure time: 20min

• WB

Lab

Western blot - HeLa whole cell lysate (AB150035)

This blot was produced using a 4-12% Bis-tris gel under the MES buffer system. The gel was run at 200V for 35 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 3% milk before being incubated with ab208842 overnight at 4°C. Antibody binding was visualised using ECL development solution ab133406.

All lanes:

Western blot - HRP Anti-CSN8 antibody [EPR5139] (<a href='/en-us/products/primary-antibodies/hrp-csn8-antibody-epr5139-ab208842'>ab208842</a>) at 1/5000 dilution

Lane 1:

Western blot - HeLa whole cell lysate (ab150035) at 10 µg

Lane 2:

Liver (Human) Tissue Lysate - fetal normal tissue at 10 µg

Lane 3:

SH-SY5Y (Human neuroblastoma cell line) Whole Cell Lysate at 10 µg

Predicted band size: 23 kDa

Observed band size: 21 kDa

true

Exposure time: 12min

• WB

Lab

Western blot - HeLa whole cell lysate (AB150035)

This blot was produced using a 4-12% Bis-tris gel under the MES buffer system. The gel was run at 200V for 35 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 3% milk before being incubated with ab197042 overnight at 4°C. Antibody binding was visualised using ECL development solution ab133406.

All lanes:

Western blot - HRP Anti-Peroxiredoxin 2/PRP antibody [EPR5154] (<a href='/en-us/products/primary-antibodies/hrp-peroxiredoxin-2-prp-antibody-epr5154-ab197042'>ab197042</a>) at 1/3000 dilution

Lane 1:

HEK-293 (Human) Whole Cell Lysate (ab52256) at 10 µg

Lane 2:

Western blot - HeLa whole cell lysate (ab150035) at 10 µg

Lane 3:

LNCaP (Human prostate adenocarcinoma cell line) Whole Cell Lysate - clone FGC at 10 µg

Lane 4:

SHSY-5Y (Human neuroblastoma cell line) Whole Cell Lysate at 10 µg

Predicted band size: 22 kDa

Observed band size: 22 kDa

true

Exposure time: 4min

• WB

Lab

Western blot - HeLa whole cell lysate (AB150035)

This blot was produced using a 4-12% Bis-tris gel under the MOPS buffer system. The gel was run at 200V for 50 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 2% Bovine Serum Albumin before being incubated with ab195857 overnight at 4°C. Antibody binding was visualised using ECL development solution ab133406.

All lanes:

Western blot - HRP Anti-YAP1 antibody [EP1674Y] (<a href='/en-us/products/primary-antibodies/hrp-yap1-antibody-ep1674y-ab195857'>ab195857</a>) at 1/5000 dilution

All lanes:

Western blot - HeLa whole cell lysate (ab150035) at 10 µg

Predicted band size: 54 kDa

Observed band size: 72 kDa

true

Exposure time: 20min

• WB

Lab

Western blot - HeLa whole cell lysate (AB150035)

This blot was produced using a 4-12% Bis-tris gel under the MES buffer system. The gel was run at 200V for 35 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 3% milk before being incubated with ab195012 overnight at 4°C. Antibody binding was visualised using ECL development solution ab133406.

All lanes:

Western blot - HRP Anti-HMGB1 antibody [EPR3507] (<a href='/en-us/products/primary-antibodies/hrp-hmgb1-antibody-epr3507-ab195012'>ab195012</a>) at 1/5000 dilution

Lane 1:

SKBR-3 whole cell lysate at 10 µg

Lane 2:

Western blot - HeLa whole cell lysate (ab150035) at 10 µg

Lane 3:

HepG2 (Human hepatocellular liver carcinoma cell line) Whole Cell Lysate at 10 µg

Predicted band size: 24 kDa

Observed band size: 28 kDa

true

Exposure time: 10s

• WB

Lab

Western blot - HeLa whole cell lysate (AB150035)

This blot was produced using a 3-8% Tris Acetate gel under the TA buffer system. The gel was run at 150V for 60 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 2% Bovine Serum Albumin before being incubated with ab206086 overnight at 4°C. Antibody binding was visualised using ECL development solution ab133406.

All lanes:

Western blot - HRP Anti-ADAR1 antibody [EPR7033] (<a href='/en-us/products/primary-antibodies/hrp-adar1-antibody-epr7033-ab206086'>ab206086</a>) at 1/5000 dilution

Lane 1:

Western blot - HeLa whole cell lysate (ab150035) at 10 µg

Lane 2:

Wild-type HAP1 cell lysate at 20 µg

Lane 3:

ADAR1 knockout HAP1 cell lysate at 20 µg

Predicted band size: 136 kDa

Observed band size: 150 kDa

true

Exposure time: 12min

• WB

Lab

Western blot - HeLa whole cell lysate (AB150035)

This blot was produced using a 4-12% Bis-tris gel under the MOPS buffer system. The gel was run at 200V for 50 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 3% milk before being incubated with ab199550 overnight at 4°C. Antibody binding was visualised using ECL development solution ab133406.

All lanes:

Western blot - HRP Anti-Amyloid Precursor Protein antibody [Y188] (<a href='/en-us/products/primary-antibodies/hrp-amyloid-precursor-protein-antibody-y188-ab199550'>ab199550</a>) at 1/5000 dilution

All lanes:

Western blot - HeLa whole cell lysate (ab150035) at 10 µg

Predicted band size: 86 kDa

Observed band size: 102 kDa

true

Exposure time: 2min

• WB

Lab

Western blot - HeLa whole cell lysate (AB150035)

This blot was produced using a 4-12% Bis-tris gel under the MOPS buffer system. The gel was run at 200V for 50 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 2% Bovine Serum Albumin before being incubated with ab193977 overnight at 4°C. Antibody binding was visualised using ECL development solution ab133406.

All lanes:

Western blot - HRP Anti-Cyclin B1 antibody [Y106] (<a href='/en-us/products/primary-antibodies/hrp-cyclin-b1-antibody-y106-ab193977'>ab193977</a>) at 1/5000 dilution

Lane 1:

Western blot - HeLa whole cell lysate (ab150035) at 10 µg

Lane 2:

Jurkat (Human T cell lymphoblast-like cell line) Whole Cell Lysate at 10 µg

Predicted band size: 48 kDa

Observed band size: 58 kDa

true

Exposure time: 1min

• WB

Lab

Western blot - HeLa whole cell lysate (AB150035)

This blot was produced using a 4-12% Bis-tris gel under the MOPS buffer system. The gel was run at 200V for 50 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 2% Bovine Serum Albumin before being incubated with ab199072 overnight at 4°C. Antibody binding was visualised using ECL development solution ab133406.

All lanes:

Western blot - HRP Anti-alpha Actinin 4 antibody [EPR2533(2)] - Loading Control (<a href='/en-us/products/primary-antibodies/hrp-alpha-actinin-4-antibody-epr25332-loading-control-ab199072'>ab199072</a>) at 1/5000 dilution

Lane 1:

A431 (Human epithelial carcinoma cell line) Whole Cell Lysate at 10 µg

Lane 2:

MCF7 (Human breast adenocarcinoma cell line) Whole Cell Lysate at 10 µg

Predicted band size: 105 kDa

Observed band size: 105 kDa

true

Exposure time: 20s

• WB

Lab

Western blot - HeLa whole cell lysate (AB150035)

This blot was produced using a 4-12% Bis-tris gel under the MOPS buffer system. The gel was run at 200V for 50 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 3% milk before being incubated with ab205733 overnight at 4°C. Antibody binding was visualised using ECL development solution ab133406.

All lanes:

Western blot - HRP Anti-pro Caspase-3 antibody [E61] (<a href='/en-us/products/primary-antibodies/hrp-pro-caspase-3-antibody-e61-ab205733'>ab205733</a>) at 1/5000 dilution

Lane 1:

Western blot - HeLa whole cell lysate (ab150035) at 10 µg

Lane 2:

Wild-type HAP1 cell lysate at 20 µg

Lane 3:

Wild-type HAP1 cell lysate Stuarosporine Treated (1μM for 4h) at 20 µg

Lane 4:

Caspase-3 knockout HAP1 cell lysate at 20 µg

Lane 5:

Caspase-3 knockout HAP1 cell lysate Stuarosporine Treated (1μM for 4h) at 20 µg

Predicted band size: 31 kDa

Observed band size: 35 kDa

true

Exposure time: 3min

• WB

Lab

Western blot - HeLa whole cell lysate (AB150035)

This blot was produced using a 4-12% Bis-tris gel under the MES buffer system. The gel was run at 200V for 35 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 3% milk before being incubated with ab208862 overnight at 4°C. Antibody binding was visualised using ECL development solution ab133406.

All lanes:

Western blot - HRP Anti-BRMS1 antibody [EPR7202] (<a href='/en-us/products/primary-antibodies/hrp-brms1-antibody-epr7202-ab208862'>ab208862</a>) at 1/5000 dilution

All lanes:

Western blot - HeLa whole cell lysate (ab150035) at 10 µg

Predicted band size: 28 kDa

Observed band size: 33 kDa

true

Exposure time: 20min

• WB

Lab

Western blot - HeLa whole cell lysate (AB150035)

This blot was produced using a 4-12% Bis-tris gel under the MOPS buffer system. The gel was run at 200V for 50 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 3% milk before being incubated with ab209049 overnight at 4°C. Antibody binding was visualised using ECL development solution ab133406.

All lanes:

Western blot - HRP Anti-FUBP1/FBP antibody [EPR12326] (<a href='/en-us/products/primary-antibodies/hrp-fubp1-fbp-antibody-epr12326-ab209049'>ab209049</a>) at 1/5000 dilution

Lane 1:

Western blot - HeLa whole cell lysate (ab150035) at 10 µg

Lane 2:

Jurkat (Human T cell lymphoblast-like cell line) Whole Cell Lysate at 10 µg

Lane 3:

HepG2 (Human hepatocellular liver carcinoma cell line) Whole Cell Lysate at 10 µg

Predicted band size: 68 kDa

Observed band size: 77 kDa

true

Exposure time: 20min

• WB

Lab

Western blot - HeLa whole cell lysate (AB150035)

This blot was produced using a 4-12% Bis-tris gel under the MOPS buffer system. The gel was run at 200V for 50 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 3% milk before being incubated with ab205443 overnight at 4°C. Antibody binding was visualised using ECL development solution ab133406.

All lanes:

Western blot - HRP Anti-IRF3 antibody [EPR2418Y] (<a href='/en-us/products/primary-antibodies/hrp-irf3-antibody-epr2418y-ab205443'>ab205443</a>) at 1/2000 dilution

Lane 1:

Western blot - HeLa whole cell lysate (ab150035) at 10 µg

Lane 2:

Jurkat (Human T cell lymphoblast-like cell line) Whole Cell Lysate at 10 µg

Lane 3:

Wild-type HAP1 cell lysate at 20 µg

Lane 4:

IRF3 knockout HAP1 cell lysate at 20 µg

Predicted band size: 47 kDa

Observed band size: 51 kDa

true

Exposure time: 20min

• WB

Lab

Western blot - HeLa whole cell lysate (AB150035)

This blot was produced using a 4-12% Bis-tris gel under the MOPS buffer system. The gel was run at 200V for 50 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 3% milk before being incubated with ab204290 overnight at 4°C. Antibody binding was visualised using ECL development solution ab133406.

All lanes:

Western blot - HRP Anti-VCP antibody [EPR3307(2)] (<a href='/en-us/products/primary-antibodies/hrp-vcp-antibody-epr33072-ab204290'>ab204290</a>) at 1/5000 dilution

Lane 1:

MCF-7 (Human breast adenocarcinoma cell line) Whole Cell Lysate at 10 µg

Lane 2:

Western blot - HeLa whole cell lysate (ab150035) at 10 µg

Lane 3:

A549 (Human lung adenocarcinoma epithelial cell line) Whole Cell Lysate at 10 µg

Lane 4:

SH-SY5Y (Human neuroblastoma cell line) Whole Cell Lysate at 10 µg

Predicted band size: 89 kDa

Observed band size: 100 kDa

true

Exposure time: 10s

• WB

Lab

Western blot - HeLa whole cell lysate (AB150035)

This blot was produced using a 4-12% Bis-tris gel under the MOPS buffer system. The gel was run at 200V for 50 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 3% milk before being incubated with ab197040 overnight at 4°C. Antibody binding was visualised using ECL development solution ab133406.

All lanes:

Western blot - HRP Anti-p73 antibody [EP436Y] (<a href='/en-us/products/primary-antibodies/hrp-p73-antibody-ep436y-ab197040'>ab197040</a>) at 1/3000 dilution

Lane 1:

Western blot - HeLa whole cell lysate (ab150035) at 10 µg

Lane 2:

Jurkat (Human T cell lymphoblast-like cell line) Whole Cell Lysate at 10 µg

Lane 3:

NIH 3T3 (Mouse embryonic fibroblast cell line) Whole Cell Lysate at 10 µg

Predicted band size: 69 kDa

Observed band size: 63 kDa

true

Exposure time: 16min

• WB

Lab

Western blot - HeLa whole cell lysate (AB150035)

This blot was produced using a 3-8% Tris Acetate gel under the TA buffer system. The gel was run at 150V for 60 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 2% Bovine Serum Albumin before being incubated with ab196315 overnight at 4°C. Antibody binding was visualised using ECL development solution ab133406.

All lanes:

Western blot - HRP Anti-BRG1 antibody [EPNCIR111A] (<a href='/en-us/products/primary-antibodies/hrp-brg1-antibody-epncir111a-ab196315'>ab196315</a>) at 1/5000 dilution

Lane 1:

K562 (Human erythromyeloblastoid leukemia cell line) Whole Cell Lysate at 10 µg

Lane 2:

Western blot - HeLa whole cell lysate (ab150035) at 10 µg

Lane 3:

NIH 3T3 (Mouse embryonic fibroblast cell line) Whole Cell Lysate at 10 µg

Lane 4:

PC12 (Rat adrenal pheochromocytoma cell line) Whole Cell Lysate at 10 µg

Predicted band size: 185 kDa

Observed band size: 185 kDa

true

Exposure time: 20min

• WB

Lab

Western blot - HeLa whole cell lysate (AB150035)

This blot was produced using a 4-12% Bis-tris gel under the MOPS buffer system. The gel was run at 200V for 50 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 3% milk before being incubated with ab190574 overnight at 4°C. Antibody binding was visualised using ECL development solution ab133406.

All lanes:

Western blot - HRP Anti-beta III Tubulin antibody [EP1569Y] - Neuronal Marker (<a href='/en-us/products/primary-antibodies/hrp-beta-iii-tubulin-antibody-ep1569y-neuronal-marker-ab190574'>ab190574</a>) at 1/3000 dilution

Lane 1:

Western blot - HeLa whole cell lysate (ab150035) at 20 µg

Lane 2:

PC12 (Rat adrenal pheochromocytoma cell line) Whole Cell Lysate at 20 µg

Predicted band size: 50 kDa

Observed band size: 52 kDa

true

Exposure time: 1s

• WB

Lab

Western blot - HeLa whole cell lysate (AB150035)

This blot was produced using a 4-12% Bis-tris gel under the MES buffer system. The gel was run at 200V for 35 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 3% milk before being incubated with ab185063 overnight at 4°C. Antibody binding was visualised using ECL development solution ab133406.

All lanes:

Western blot - HRP Anti-VDAC1/Porin + VDAC2 antibody [EPR10852(B)] - Mitochondrial Loading Control (<a href='/en-us/products/primary-antibodies/hrp-vdac1-porin-vdac2-antibody-epr10852b-mitochondrial-loading-control-ab185063'>ab185063</a>) at 1/5000 dilution

Lane 1:

HepG2 (Human hepatocellular liver carcinoma cell line) Whole Cell Lysate at 10 µg

Lane 2:

Western blot - HeLa whole cell lysate (ab150035) at 10 µg

Lane 3:

Jurkat (Human T cell lymphoblast-like cell line) Whole Cell Lysate at 10 µg

Lane 4:

Kidney (Mouse) Tissue Lysate at 10 µg

Lane 5:

Kidney (Rat) Tissue Lysate at 10 µg

Lane 6:

Kidney (Human) Tissue Lysate - adult normal tissue at 10 µg

Observed band size: 35 kDa

true

Exposure time: 4min

• WB

Lab

Western blot - HeLa whole cell lysate (AB150035)

This blot was produced using a 4-12% Bis-tris gel under the MES buffer system. The gel was run at 200V for 35 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 3% milk before being incubated with ab205822 overnight at 4°C. Antibody binding was visualised using ECL development solution ab133406.

All lanes:

Western blot - HRP Anti-Bax antibody [E63] (<a href='/en-us/products/primary-antibodies/hrp-bax-antibody-e63-ab205822'>ab205822</a>) at 1/5000 dilution

Lane 1:

Western blot - HeLa whole cell lysate (ab150035) at 10 µg

Lane 2:

Wild-type HAP1 cell lysate at 20 µg

Lane 3:

Bax knockout HAP1 cell lysate at 20 µg

Predicted band size: 21 kDa

Observed band size: 19 kDa

true

Exposure time: 8min

• WB

Lab

Western blot - HeLa whole cell lysate (AB150035)

This blot was produced using a 4-12% Bis-tris gel under the MOPS buffer system. The gel was run at 200V for 50 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 3% milk before being incubated with ab204022 overnight at 4°C. Antibody binding was visualised using ECL development solution ab133406.

All lanes:

Western blot - HRP Anti-Cytochrome P450 17A1/CYP17A1 antibody [EPR6293] (<a href='/en-us/products/primary-antibodies/hrp-cytochrome-p450-17a1-cyp17a1-antibody-epr6293-ab204022'>ab204022</a>) at 1/5000 dilution

Lane 1:

Western blot - HeLa whole cell lysate (ab150035) at 10 µg

Lane 2:

Jurkat (Human T cell lymphoblast-like cell line) Whole Cell Lysate at 10 µg

Predicted band size: 57 kDa

Observed band size: 57 kDa

true

Exposure time: 3min