HeLa whole cell lysate

39 product images

• 

  Western blot - HeLa whole cell lysate (ab150035)

  This blot was produced using a 4-12% Bis-tris gel under the MOPS buffer system. The gel was run at 200V for 50 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 2% Bovine Serum Albumin before being incubated with HRP Anti-beta IV Tubulin antibody [ONS.1A6] ab204454 overnight at 4°C. Antibody binding was visualised using ECL development solution ECL Substrate Kit (High Sensitivity) ab133406.

  All lanes: Western blot - HRP Anti-beta IV Tubulin antibody [ONS.1A6] (HRP Anti-beta IV Tubulin antibody [ONS.1A6] ab204454) at 1/5000 dilution

  Lane 1: Western blot - HeLa whole cell lysate (ab150035) at 10 µg

  Lane 2: HEK293 (Human embryonic kidney cell line) Whole Cell Lysate at 10 µg

  Lane 3: NIH 3T3 (Mouse embryonic fibroblast cell line) Whole Cell Lysate at 10 µg

  Developed using the ECL technique.

  Performed under reducing conditions.

  Predicted band size: 50 kDa

  Observed band size: 52 kDa

  Exposure time: 20s

• 

  Western blot - HeLa whole cell lysate (ab150035)

  This blot was produced using a 4-12% Bis-tris gel under the MES buffer system. The gel was run at 200V for 35 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 3% milk before being incubated with HRP Anti-VDAC1/Porin + VDAC2 antibody [EPR10852(B)] - Mitochondrial Loading Control ab185063 overnight at 4°C. Antibody binding was visualised using ECL development solution ECL Substrate Kit (High Sensitivity) ab133406.

  All lanes: Western blot - HRP Anti-VDAC1/Porin + VDAC2 antibody [EPR10852(B)] - Mitochondrial Loading Control (HRP Anti-VDAC1/Porin + VDAC2 antibody [EPR10852(B)] - Mitochondrial Loading Control ab185063) at 1/5000 dilution

  Lane 1: HepG2 (Human hepatocellular liver carcinoma cell line) Whole Cell Lysate at 10 µg

  Lane 2: Western blot - HeLa whole cell lysate (ab150035) at 10 µg

  Lane 3: Jurkat (Human T cell lymphoblast-like cell line) Whole Cell Lysate at 10 µg

  Lane 4: Kidney (Mouse) Tissue Lysate at 10 µg

  Lane 5: Kidney (Rat) Tissue Lysate at 10 µg

  Lane 6: Kidney (Human) Tissue Lysate - adult normal tissue at 10 µg

  Developed using the ECL technique.

  Performed under reducing conditions.

  Observed band size: 35 kDa

  Exposure time: 4min

• 

  Western blot - HeLa whole cell lysate (ab150035)

  This blot was produced using a 4-12% Bis-tris gel under the MOPS buffer system. The gel was run at 200V for 50 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 2% Bovine Serum Albumin before being incubated with HRP Anti-Tubulin antibody [YOL1/34] - Microtubule Marker ab196583 overnight at 4°C. Antibody binding was visualised using ECL development solution ECL Substrate Kit (High Sensitivity) ab133406.

  All lanes: Western blot - HRP Anti-Tubulin antibody [YOL1/34] - Microtubule Marker (HRP Anti-Tubulin antibody [YOL1/34] - Microtubule Marker ab196583) at 1/5000 dilution

  Lane 1: Western blot - HeLa whole cell lysate (ab150035) at 20 µg

  Lane 2: NIH 3T3 (Mouse embryonic fibroblast cell line) Whole Cell Lysate at 20 µg

  Lane 3: Brain (Rat) Tissue Lysate at 20 µg

  Developed using the ECL technique.

  Performed under reducing conditions.

  Predicted band size: 50 kDa

  Observed band size: 50 kDa

  Exposure time: 2s

• 

  Western blot - HeLa whole cell lysate (ab150035)

  This blot was produced using a 4-12% Bis-tris gel under the MOPS buffer system. The gel was run at 200V for 50 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 2% Bovine Serum Albumin before being incubated with HRP Anti-hnRNP A1 antibody [EPR12768] ab198535 overnight at 4°C. Antibody binding was visualised using ECL development solution ECL Substrate Kit (High Sensitivity) ab133406.

  All lanes: Western blot - HRP Anti-hnRNP A1 antibody [EPR12768] (HRP Anti-hnRNP A1 antibody [EPR12768] ab198535) at 1/5000 dilution

  Lane 1: MCF-7 (Human breast adenocarcinoma cell line) Whole Cell Lysate at 10 µg

  Lane 2: HepG2 (Human hepatocellular liver carcinoma cell line) Whole Cell Lysate at 10 µg

  Lane 3: K562 (Human erythromyeloblastoid leukemia cell line) Whole Cell Lysate at 10 µg

  Lane 4: Western blot - HeLa whole cell lysate (ab150035) at 10 µg

  Lane 5: Jurkat (Human T cell lymphoblast-like cell line) Whole Cell Lysate at 10 µg

  Developed using the ECL technique.

  Performed under reducing conditions.

  Predicted band size: 38 kDa

  Observed band size: 34 kDa

  Exposure time: 14s

• 

  Western blot - HeLa whole cell lysate (ab150035)

  This blot was produced using a 4-12% Bis-tris gel under the MOPS buffer system. The gel was run at 200V for 50 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 3% milk before being incubated with HRP Anti-Sodium Potassium ATPase antibody [EP1845Y] - Plasma Membrane Loading Control ab185065 overnight at 4°C. Antibody binding was visualised using ECL development solution ECL Substrate Kit (High Sensitivity) ab133406.

  All lanes: Western blot - HRP Anti-Sodium Potassium ATPase antibody [EP1845Y] - Plasma Membrane Loading Control (HRP Anti-Sodium Potassium ATPase antibody [EP1845Y] - Plasma Membrane Loading Control ab185065) at 1/5000 dilution

  Lane 1: Western blot - HeLa whole cell lysate (ab150035) at 10 µg

  Lane 2: HEK293 (Human embryonic kidney cell line) Whole Cell Lysate at 10 µg

  Developed using the ECL technique.

  Performed under reducing conditions.

  Predicted band size: 113 kDa

  Observed band size: 100 kDa

  Exposure time: 30s

• 

  Western blot - HeLa whole cell lysate (ab150035)

  This blot was produced using a 4-12% Bis-tris gel under the MOPS buffer system. The gel was run at 200V for 50 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 3% milk before being incubated with HRP Anti-IRF3 antibody [EPR2418Y] ab205443 overnight at 4°C. Antibody binding was visualised using ECL development solution ECL Substrate Kit (High Sensitivity) ab133406.

  All lanes: Western blot - HRP Anti-IRF3 antibody [EPR2418Y] (HRP Anti-IRF3 antibody [EPR2418Y] ab205443) at 1/2000 dilution

  Lane 1: Western blot - HeLa whole cell lysate (ab150035) at 10 µg

  Lane 2: Jurkat (Human T cell lymphoblast-like cell line) Whole Cell Lysate at 10 µg

  Lane 3: Wild-type HAP1 cell lysate at 20 µg

  Lane 4: IRF3 knockout HAP1 cell lysate at 20 µg

  Developed using the ECL technique.

  Performed under reducing conditions.

  Predicted band size: 47 kDa

  Observed band size: 51 kDa

  Exposure time: 20min

• 

  Western blot - HeLa whole cell lysate (ab150035)

  This blot was produced using a 4-12% Bis-tris gel under the MOPS buffer system. The gel was run at 200V for 50 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 3% milk before being incubated with HRP Anti-ASH2L antibody [EPR13106] ab209317 overnight at 4°C. Antibody binding was visualised using ECL development solution ECL Substrate Kit (High Sensitivity) ab133406.

  All lanes: Western blot - HRP Anti-ASH2L antibody [EPR13106] (HRP Anti-ASH2L antibody [EPR13106] ab209317) at 1/3000 dilution

  Lane 1: HEK293 (Human embryonic kidney cell line) Whole Cell Lysate at 10 µg

  Lane 2: Western blot - HeLa whole cell lysate (ab150035) at 10 µg

  Lane 3: C6 (Rat glioma cell line) Whole Cell Lysate at 10 µg

  Lane 4: PC12 (Rat adrenal pheochromocytoma cell line) Whole Cell Lysate at 10 µg

  Developed using the ECL technique.

  Performed under reducing conditions.

  Predicted band size: 69 kDa

  Observed band size: 80 kDa

  Exposure time: 20min

• 

  Western blot - HeLa whole cell lysate (ab150035)

  This blot was produced using a 4-12% Bis-tris gel under the MOPS buffer system. The gel was run at 200V for 50 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 3% milk before being incubated with HRP Anti-Cyclin E1 antibody [EP435E] ab194070 overnight at 4°C. Antibody binding was visualised using ECL development solution ECL Substrate Kit (High Sensitivity) ab133406.

  All lanes: HRP Anti-Cyclin E1 antibody [EP435E] (HRP Anti-Cyclin E1 antibody [EP435E] ab194070) at 1/5000 dilution

  All lanes: Western blot - HeLa whole cell lysate (ab150035) at 20 µg

  Performed under reducing conditions.

  Exposure time: 20min

• 

  Western blot - HeLa whole cell lysate (ab150035)

  This blot was produced using a 4-12% Bis-tris gel under the MOPS buffer system. The gel was run at 200V for 50 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 2% Bovine Serum Albumin before being incubated with HRP Anti-PPP1CA + PPP1CB antibody [EP1511Y] ab211372 overnight at 4°C. Antibody binding was visualised using ECL development solution ECL Substrate Kit (High Sensitivity) ab133406.

  All lanes: Western blot - HRP Anti-PPP1CA + PPP1CB antibody [EP1511Y] (HRP Anti-PPP1CA + PPP1CB antibody [EP1511Y] ab211372) at 1/5000 dilution

  Lane 1: Brain (Mouse) Tissue Lysate at 10 µg

  Lane 2: Brain (Rat) Tissue Lysate at 10 µg

  Lane 3: Western blot - HeLa whole cell lysate (ab150035) at 10 µg

  Developed using the ECL technique.

  Performed under reducing conditions.

  Predicted band size: 37 kDa

  Observed band size: 36 kDa

  Exposure time: 3min

• 

  Western blot - HeLa whole cell lysate (ab150035)

  This blot was produced using a 4-12% Bis-tris gel under the MOPS buffer system. The gel was run at 200V for 50 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 2% Bovine Serum Albumin before being incubated with HRP Anti-ATPB antibody [3D5] - Mitochondrial Marker ab197905 overnight at 4°C. Antibody binding was visualised using ECL development solution ECL Substrate Kit (High Sensitivity) ab133406.

  All lanes: Western blot - HRP Anti-ATPB antibody [3D5] - Mitochondrial Marker (HRP Anti-ATPB antibody [3D5] - Mitochondrial Marker ab197905) at 1/5000 dilution

  Lane 1: Human heart tissue lysate - mitochondrial extract (ab110337) at 5 µg

  Lane 2: Western blot - HeLa whole cell lysate (ab150035) at 10 µg

  Lane 3: HepG2 (Human hepatocellular liver carcinoma cell line) Whole Cell Lysate at 10 µg

  Developed using the ECL technique.

  Performed under reducing conditions.

  Predicted band size: 56 kDa

  Observed band size: 52 kDa

  Exposure time: 2s

• 

  Western blot - HeLa whole cell lysate (ab150035)

  This blot was produced using a 4-12% Bis-tris gel under the MOPS buffer system. The gel was run at 200V for 50 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 2% Bovine Serum Albumin before being incubated with HRP Anti-alpha Actinin 4 antibody [EPR2533(2)] ab199072 overnight at 4°C. Antibody binding was visualised using ECL development solution ECL Substrate Kit (High Sensitivity) ab133406.

  All lanes: Western blot - HRP Anti-alpha Actinin 4 antibody [EPR2533(2)] (HRP Anti-alpha Actinin 4 antibody [EPR2533(2)] ab199072) at 1/5000 dilution

  Lane 1: A431 (Human epithelial carcinoma cell line) Whole Cell Lysate at 10 µg

  Lane 2: MCF7 (Human breast adenocarcinoma cell line) Whole Cell Lysate at 10 µg

  Developed using the ECL technique.

  Performed under reducing conditions.

  Predicted band size: 105 kDa

  Observed band size: 105 kDa

  Exposure time: 20s

• 

  Western blot - HeLa whole cell lysate (ab150035)

  This blot was produced using a 4-12% Bis-tris gel under the MOPS buffer system. The gel was run at 200V for 50 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 3% milk before being incubated with HRP Anti-PGC1 beta antibody [EPR12370] ab199228 overnight at 4°C. Antibody binding was visualised using ECL development solution ECL Substrate Kit (High Sensitivity) ab133406.

  All lanes: Western blot - HRP Anti-PGC1 beta antibody [EPR12370] (HRP Anti-PGC1 beta antibody [EPR12370] ab199228) at 1/5000 dilution

  Lane 1: Western blot - HeLa whole cell lysate (ab150035) at 10 µg

  Lane 2: MCF7 (Human breast adenocarcinoma cell line) Whole Cell Lysate at 10 µg

  Lane 3: Brain (Human) Tissue Lysate - fetal normal tissue at 10 µg

  Lane 4: Heart (Human) Tissue Lysate - fetal normal tissue at 10 µg

  Developed using the ECL technique.

  Performed under reducing conditions.

  Predicted band size: 113 kDa

  Observed band size: 125 kDa

  Exposure time: 10s

• 

  Western blot - HeLa whole cell lysate (ab150035)

  This blot was produced using a 4-12% Bis-tris gel under the MES buffer system. The gel was run at 200V for 35 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 3% milk before being incubated with HRP Anti-mtTFA antibody [EPR12285] - Mitochondrial Marker ab209022 overnight at 4°C. Antibody binding was visualised using ECL development solution ECL Substrate Kit (High Sensitivity) ab133406.

  All lanes: Western blot - HRP Anti-mtTFA antibody [EPR12285] - Mitochondrial Marker (HRP Anti-mtTFA antibody [EPR12285] - Mitochondrial Marker ab209022) at 1/5000 dilution

  Lane 1: Western blot - HeLa whole cell lysate (ab150035) at 10 µg

  Lane 2: MCF-7 (Human breast adenocarcinoma cell line) Whole Cell Lysate at 10 µg

  Lane 3: HEK293 (Human embryonic kidney cell line) Whole Cell Lysate at 10 µg

  Developed using the ECL technique.

  Performed under reducing conditions.

  Predicted band size: 29 kDa

  Observed band size: 26 kDa

  Exposure time: 20min

• 

  Western blot - HeLa whole cell lysate (ab150035)

  This blot was produced using a 4-12% Bis-tris gel under the MOPS buffer system. The gel was run at 200V for 50 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 3% milk before being incubated with HRP Anti-p73 antibody [EP436Y] ab197040 overnight at 4°C. Antibody binding was visualised using ECL development solution ECL Substrate Kit (High Sensitivity) ab133406.

  All lanes: Western blot - HRP Anti-p73 antibody [EP436Y] (HRP Anti-p73 antibody [EP436Y] ab197040) at 1/3000 dilution

  Lane 1: Western blot - HeLa whole cell lysate (ab150035) at 10 µg

  Lane 2: Jurkat (Human T cell lymphoblast-like cell line) Whole Cell Lysate at 10 µg

  Lane 3: NIH 3T3 (Mouse embryonic fibroblast cell line) Whole Cell Lysate at 10 µg

  Developed using the ECL technique.

  Performed under reducing conditions.

  Predicted band size: 69 kDa

  Observed band size: 63 kDa

  Exposure time: 16min

• 

  Western blot - HeLa whole cell lysate (ab150035)

  This blot was produced using a 3-8% Tris Acetate gel under the TA buffer system. The gel was run at 150V for 60 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 2% Bovine Serum Albumin before being incubated with HRP Anti-Brd4 antibody [EPR5150(2)] ab197609 overnight at 4°C. Antibody binding was visualised using ECL development solution ECL Substrate Kit (High Sensitivity) ab133406.

  All lanes: Western blot - HRP Anti-Brd4 antibody [EPR5150(2)] (HRP Anti-Brd4 antibody [EPR5150(2)] ab197609) at 1/5000 dilution

  Lane 1: Western blot - HeLa whole cell lysate (ab150035) at 10 µg

  Lane 2: Caco 2 (Human colonic carcinoma cell line) Whole Cell Lysate at 10 µg

  Lane 3: RAW 264.7 (Mouse leukaemic monocyte macrophage cell line) Whole Cell Lysate at 10 µg

  Lane 4: NIH 3T3 (Mouse embryonic fibroblast cell line) Whole Cell Lysate at 10 µg

  Developed using the ECL technique.

  Performed under reducing conditions.

  Predicted band size: 152 kDa

  Observed band size: 152 kDa

  Exposure time: 2min

• 

  Western blot - HeLa whole cell lysate (ab150035)

  This blot was produced using a 4-12% Bis-tris gel under the MOPS buffer system. The gel was run at 200V for 50 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 3% milk before being incubated with HRP Anti-Fibrillarin antibody [EPR10823(B)] - Nucleolar Marker ab196980 overnight at 4°C. Antibody binding was visualised using ECL development solution ECL Substrate Kit (High Sensitivity) ab133406.

  All lanes: Western blot - HRP Anti-Fibrillarin antibody [EPR10823(B)] - Nucleolar Marker (HRP Anti-Fibrillarin antibody [EPR10823(B)] - Nucleolar Marker ab196980) at 1/5000 dilution

  Lane 1: HEK-293 (Human) Whole Cell Lysate (ab52256) at 10 µg

  Lane 2: Western blot - HeLa whole cell lysate (ab150035) at 10 µg

  Lane 3: HepG2 (Human hepatocellular liver carcinoma cell line) Whole Cell Lysate at 10 µg

  Lane 4: MOLT4 (Human acute lymphoblastic leukemia cell line) Whole Cell Lysate at 10 µg

  Developed using the ECL technique.

  Performed under reducing conditions.

  Predicted band size: 33 kDa

  Observed band size: 33 kDa

  Exposure time: 8min

• 

  Western blot - HeLa whole cell lysate (ab150035)

  This blot was produced using a 4-12% Bis-tris gel under the MES buffer system. The gel was run at 200V for 35 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 3% milk before being incubated with ab208369 overnight at 4°C. Antibody binding was visualised using ECL development solution ECL Substrate Kit (High Sensitivity) ab133406.

  All lanes: HRP Anti-Superoxide Dismutase 1 antibody [EPR1726] (ab208369) at 1/5000 dilution

  Lane 1: Western blot - HeLa whole cell lysate (ab150035) at 10 µg

  Lane 2: MCF-7 (Human breast adenocarcinoma cell line) Whole Cell Lysate at 10 µg

  Lane 3: Jurkat (Human T cell lymphoblast-like cell line) Whole Cell Lysate at 10 µg

  Lane 4: HT29 (Human colon adenocarcinoma grade II cell line) Whole Cell Lysate at 10 µg

  Developed using the ECL technique.

  Performed under reducing conditions.

  Exposure time: 4min

• 

  Western blot - HeLa whole cell lysate (ab150035)

  This blot was produced using a 4-12% Bis-tris gel under the MOPS buffer system. The gel was run at 200V for 50 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 3% milk before being incubated with HRP Anti-delta 1 Catenin/CAS antibody [YE372] ab202914 overnight at 4°C. Antibody binding was visualised using ECL development solution ECL Substrate Kit (High Sensitivity) ab133406.

  All lanes: Western blot - HRP Anti-delta 1 Catenin/CAS antibody [YE372] (HRP Anti-delta 1 Catenin/CAS antibody [YE372] ab202914) at 1/5000 dilution

  All lanes: Western blot - HeLa whole cell lysate (ab150035) at 10 µg

  Developed using the ECL technique.

  Performed under reducing conditions.

  Predicted band size: 108 kDa

  Observed band size: 120 kDa

  Exposure time: 1min

• 

  Western blot - HeLa whole cell lysate (ab150035)

  This blot was produced using a 4-12% Bis-tris gel under the MES buffer system. The gel was run at 200V for 35 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 3% milk before being incubated with HRP Anti-BRMS1 antibody [EPR7202] ab208862 overnight at 4°C. Antibody binding was visualised using ECL development solution ECL Substrate Kit (High Sensitivity) ab133406.

  All lanes: Western blot - HRP Anti-BRMS1 antibody [EPR7202] (HRP Anti-BRMS1 antibody [EPR7202] ab208862) at 1/5000 dilution

  All lanes: Western blot - HeLa whole cell lysate (ab150035) at 10 µg

  Developed using the ECL technique.

  Performed under reducing conditions.

  Predicted band size: 28 kDa

  Observed band size: 33 kDa

  Exposure time: 20min

• 

  Western blot - HeLa whole cell lysate (ab150035)

  This blot was produced using a 4-12% Bis-tris gel under the MOPS buffer system. The gel was run at 200V for 50 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 3% milk before being incubated with HRP Anti-Cyclin A2 antibody [E399] ab193598 overnight at 4°C. Antibody binding was visualised using ECL development solution ECL Substrate Kit (High Sensitivity) ab133406.

  All lanes: Western blot - HRP Anti-Cyclin A2 antibody [E399] (HRP Anti-Cyclin A2 antibody [E399] ab193598) at 1/1000 dilution

  All lanes: Western blot - HeLa whole cell lysate (ab150035) at 10 µg

  Developed using the ECL technique.

  Performed under reducing conditions.

  Predicted band size: 49 kDa

  Observed band size: 52 kDa

  Exposure time: 20min

• 

  Western blot - HeLa whole cell lysate (ab150035)

  This blot was produced using a 4-12% Bis-tris gel under the MOPS buffer system. The gel was run at 200V for 50 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 3% milk before being incubated with HRP Anti-Amyloid Precursor Protein antibody [Y188] ab199550 overnight at 4°C. Antibody binding was visualised using ECL development solution ECL Substrate Kit (High Sensitivity) ab133406.

  All lanes: Western blot - HRP Anti-Amyloid Precursor Protein antibody [Y188] (HRP Anti-Amyloid Precursor Protein antibody [Y188] ab199550) at 1/5000 dilution

  All lanes: Western blot - HeLa whole cell lysate (ab150035) at 10 µg

  Developed using the ECL technique.

  Performed under reducing conditions.

  Predicted band size: 86 kDa

  Observed band size: 102 kDa

  Exposure time: 2min

• 

  Western blot - HeLa whole cell lysate (ab150035)

  This blot was produced using a 4-12% Bis-tris gel under the MOPS buffer system. The gel was run at 200V for 50 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 2% Bovine Serum Albumin before being incubated with HRP Anti-67kDa Laminin Receptor antibody [EPR8469] ab197712 overnight at 4°C. Antibody binding was visualised using ECL development solution ECL Substrate Kit (High Sensitivity) ab133406.

  All lanes: Western blot - HRP Anti-67kDa Laminin Receptor antibody [EPR8469] (HRP Anti-67kDa Laminin Receptor antibody [EPR8469] ab197712) at 1/5000 dilution

  Lane 1: Western blot - HeLa whole cell lysate (ab150035) at 10 µg

  Lane 2: HepG2 (Human hepatocellular liver carcinoma cell line) Whole Cell Lysate at 10 µg

  Lane 3: PC12 (Rat adrenal pheochromocytoma cell line) Whole Cell Lysate at 10 µg

  Lane 4: NIH 3T3 (Mouse embryonic fibroblast cell line) Whole Cell Lysate at 10 µg

  Developed using the ECL technique.

  Performed under reducing conditions.

  Predicted band size: 33 kDa

  Observed band size: 37 kDa

  Exposure time: 12s

• 

  Western blot - HeLa whole cell lysate (ab150035)

  This blot was produced using a 4-12% Bis-tris gel under the MOPS buffer system. The gel was run at 200V for 50 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 3% milk before being incubated with HRP Anti-Lactate Dehydrogenase B/LDH-B antibody [EP1565Y] ab208366 overnight at 4°C. Antibody binding was visualised using ECL development solution ECL Substrate Kit (High Sensitivity) ab133406.

  All lanes: Western blot - HRP Anti-Lactate Dehydrogenase B/LDH-B antibody [EP1565Y] (HRP Anti-Lactate Dehydrogenase B/LDH-B antibody [EP1565Y] ab208366) at 1/5000 dilution

  All lanes: Western blot - HeLa whole cell lysate (ab150035) at 10 µg

  Developed using the ECL technique.

  Performed under reducing conditions.

  Predicted band size: 37 kDa

  Observed band size: 35 kDa

  Exposure time: 30s

• 

  Western blot - HeLa whole cell lysate (ab150035)

  This blot was produced using a 4-12% Bis-tris gel under the MOPS buffer system. The gel was run at 200V for 50 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 3% milk before being incubated with HRP Anti-FUBP1/FBP antibody [EPR12326] ab209049 overnight at 4°C. Antibody binding was visualised using ECL development solution ECL Substrate Kit (High Sensitivity) ab133406.

  All lanes: Western blot - HRP Anti-FUBP1/FBP antibody [EPR12326] (HRP Anti-FUBP1/FBP antibody [EPR12326] ab209049) at 1/5000 dilution

  Lane 1: Western blot - HeLa whole cell lysate (ab150035) at 10 µg

  Lane 2: Jurkat (Human T cell lymphoblast-like cell line) Whole Cell Lysate at 10 µg

  Lane 3: HepG2 (Human hepatocellular liver carcinoma cell line) Whole Cell Lysate at 10 µg

  Developed using the ECL technique.

  Performed under reducing conditions.

  Predicted band size: 68 kDa

  Observed band size: 77 kDa

  Exposure time: 20min

• 

  Western blot - HeLa whole cell lysate (ab150035)

  This blot was produced using a 4-12% Bis-tris gel under the MOPS buffer system. The gel was run at 200V for 50 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 2% Bovine Serum Albumin before being incubated with HRP Anti-YAP1 antibody [EP1674Y] ab195857 overnight at 4°C. Antibody binding was visualised using ECL development solution ECL Substrate Kit (High Sensitivity) ab133406.

  All lanes: Western blot - HRP Anti-YAP1 antibody [EP1674Y] (HRP Anti-YAP1 antibody [EP1674Y] ab195857) at 1/5000 dilution

  All lanes: Western blot - HeLa whole cell lysate (ab150035) at 10 µg

  Developed using the ECL technique.

  Performed under reducing conditions.

  Predicted band size: 54 kDa

  Observed band size: 72 kDa

  Exposure time: 20min

• 

  Western blot - HeLa whole cell lysate (ab150035)

  This blot was produced using a 4-12% Bis-tris gel under the MOPS buffer system. The gel was run at 200V for 50 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 5% Bovine Serum Albumin before being incubated with HRP Anti-alpha Tubulin antibody [EPR13478(B)] - Loading Control ab185067 overnight at 4°C. Antibody binding was visualised using ECL development solution ECL Substrate Kit (High Sensitivity) ab133406

  All lanes: Western blot - HRP Anti-alpha Tubulin antibody [EPR13478(B)] - Loading Control (HRP Anti-alpha Tubulin antibody [EPR13478(B)] - Loading Control ab185067) at 1/5000 dilution

  Lane 1: Western blot - HeLa whole cell lysate (ab150035) at 10 µg

  Lane 2: Jurkat (Human T cell lymphoblast-like cell line) Whole Cell Lysate at 10 µg

  Lane 3: A431 (Human epithelial carcinoma cell line) Whole Cell Lysate at 10 µg

  Lane 4: K562 (Human erythromyeloblastoid leukemia cell line) Whole Cell Lysate at 10 µg

  Developed using the ECL technique.

  Performed under reducing conditions.

  Predicted band size: 50 kDa

  Observed band size: 52 kDa

  Exposure time: 10s

• 

  Western blot - HeLa whole cell lysate (ab150035)

  This blot was produced using a 4-12% Bis-tris gel under the MES buffer system. The gel was run at 200V for 35 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 3% milk before being incubated with HRP Anti-CSN8 antibody [EPR5139] ab208842 overnight at 4°C. Antibody binding was visualised using ECL development solution ECL Substrate Kit (High Sensitivity) ab133406.

  All lanes: Western blot - HRP Anti-CSN8 antibody [EPR5139] (HRP Anti-CSN8 antibody [EPR5139] ab208842) at 1/5000 dilution

  Lane 1: Western blot - HeLa whole cell lysate (ab150035) at 10 µg

  Lane 2: Liver (Human) Tissue Lysate - fetal normal tissue at 10 µg

  Lane 3: SH-SY5Y (Human neuroblastoma cell line) Whole Cell Lysate at 10 µg

  Developed using the ECL technique.

  Performed under reducing conditions.

  Predicted band size: 23 kDa

  Observed band size: 21 kDa

  Exposure time: 12min

• 

  Western blot - HeLa whole cell lysate (ab150035)
• 

  Western blot - HeLa whole cell lysate (ab150035)

  This blot was produced using a 4-12% Bis-tris gel under the MES buffer system. The gel was run at 200V for 35 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 2% Bovine Serum Albumin before being incubated with HRP Anti-Histone H3 antibody [EPR16987] - Nuclear Loading Control ab209023 overnight at 4°C. Antibody binding was visualised using ECL development solution ECL Substrate Kit (High Sensitivity) ab133406.

  All lanes: Western blot - HRP Anti-Histone H3 antibody [EPR16987] - Nuclear Loading Control (HRP Anti-Histone H3 antibody [EPR16987] - Nuclear Loading Control ab209023) at 1/5000 dilution

  Lane 1: Western blot - HeLa whole cell lysate (ab150035) at 10 µg

  Lane 2: NIH 3T3 (Mouse embryonic fibroblast cell line) Whole Cell Lysate at 10 µg

  Lane 3: Drosophila embryo nuclear extract (from melanogaster embryos 0-12hr) at 10 µg

  Developed using the ECL technique.

  Performed under reducing conditions.

  Predicted band size: 15 kDa

  Observed band size: 17 kDa

  Exposure time: 2min

• 

  Western blot - HeLa whole cell lysate (ab150035)

  This blot was produced using a 4-12% Bis-tris gel under the MES buffer system. The gel was run at 200V for 35 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 3% milk before being incubated with HRP Anti-PCNA antibody [EPR3821] ab193965 overnight at 4°C. Antibody binding was visualised using ECL development solution ECL Substrate Kit (High Sensitivity) ab133406.

  All lanes: Western blot - HRP Anti-PCNA antibody [EPR3821] (HRP Anti-PCNA antibody [EPR3821] ab193965) at 1/5000 dilution

  Lane 1: Western blot - HeLa whole cell lysate (ab150035) at 10 µg

  Lane 2: HepG2 (Human hepatocellular liver carcinoma cell line) Whole Cell Lysate at 10 µg

  Lane 3: HEK293 (Human embryonic kidney cell line) Whole Cell Lysate at 10 µg

  Lane 4: A431 (Human epithelial carcinoma cell line) Whole Cell Lysate at 10 µg

  Developed using the ECL technique.

  Performed under reducing conditions.

  Predicted band size: 29 kDa

  Observed band size: 29 kDa

  Exposure time: 30s

• 

  Western blot - HeLa whole cell lysate (ab150035)

  This blot was produced using a 4-12% Bis-tris gel under the MES buffer system. The gel was run at 200V for 35 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 3% milk before being incubated with HRP Anti-Cdk4 antibody [EPR4513-32-7] - Loading Control ab193968 overnight at 4°C. Antibody binding was visualised using ECL development solution ECL Substrate Kit (High Sensitivity) ab133406.

  All lanes: Western blot - HRP Anti-Cdk4 antibody [EPR4513-32-7] - Loading Control (HRP Anti-Cdk4 antibody [EPR4513-32-7] - Loading Control ab193968) at 1/5000 dilution

  Lane 1: Western blot - HeLa whole cell lysate (ab150035) at 10 µg

  Lane 2: MCF7 (Human breast adenocarcinoma cell line) Whole Cell Lysate at 10 µg

  Lane 3: K562 (Human erythromyeloblastoid leukemia cell line) Whole Cell Lysate at 10 µg

  Lane 4: Ramos (Human Burkitt's lymphoma cell line) Whole Cell Lysate at 10 µg

  Developed using the ECL technique.

  Performed under reducing conditions.

  Predicted band size: 34 kDa

  Observed band size: 34 kDa

  Exposure time: 90s

• 

  Western blot - HeLa whole cell lysate (ab150035)

  This blot was produced using a 4-12% Bis-tris gel under the MOPS buffer system. The gel was run at 200V for 50 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 3% milk before being incubated with HRP Anti-beta III Tubulin antibody [EP1569Y] - Neuronal Marker ab190574 overnight at 4°C. Antibody binding was visualised using ECL development solution ECL Substrate Kit (High Sensitivity) ab133406.

  All lanes: Western blot - HRP Anti-beta III Tubulin antibody [EP1569Y] - Neuronal Marker (HRP Anti-beta III Tubulin antibody [EP1569Y] - Neuronal Marker ab190574) at 1/3000 dilution

  Lane 1: Western blot - HeLa whole cell lysate (ab150035) at 20 µg

  Lane 2: PC12 (Rat adrenal pheochromocytoma cell line) Whole Cell Lysate at 20 µg

  Developed using the ECL technique.

  Performed under reducing conditions.

  Predicted band size: 50 kDa

  Observed band size: 52 kDa

  Exposure time: 1s

• 

  Western blot - HeLa whole cell lysate (ab150035)

  This blot was produced using a 3-8% Tris Acetate gel under the TA buffer system. The gel was run at 150V for 60 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 3% milk before being incubated with HRP Anti-SF3B1 antibody [EPR11986] ab202926 overnight at 4°C. Antibody binding was visualised using ECL development solution ECL Substrate Kit (High Sensitivity) ab133406.

  All lanes: Western blot - HRP Anti-SF3B1 antibody [EPR11986] (HRP Anti-SF3B1 antibody [EPR11986] ab202926) at 1/5000 dilution

  Lane 1: Western blot - HeLa whole cell lysate (ab150035) at 10 µg

  Lane 2: Jurkat (Human T cell lymphoblast-like cell line) Whole Cell Lysate at 10 µg

  Lane 3: K562 (Human erythromyeloblastoid leukemia cell line) Whole Cell Lysate at 10 µg

  Lane 4: Ramos (Human Burkitt's lymphoma cell line) Whole Cell Lysate at 10 µg

  Developed using the ECL technique.

  Performed under reducing conditions.

  Predicted band size: 146 kDa

  Observed band size: 146 kDa

  Exposure time: 30s

• 

  Western blot - HeLa whole cell lysate (ab150035)

  This blot was produced using a 4-12% Bis-tris gel under the MOPS buffer system. The gel was run at 200V for 50 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 3% milk before being incubated with HRP Anti-SQSTM1 / p62 antibody [EPR4844] ab194720 overnight at 4°C. Antibody binding was visualised using ECL development solution ECL Substrate Kit (High Sensitivity) ab133406.

  All lanes: Western blot - HRP Anti-SQSTM1 / p62 antibody [EPR4844] (HRP Anti-SQSTM1 / p62 antibody [EPR4844] ab194720) at 1/1000 dilution

  Lane 1: MCF7 (Human breast adenocarcinoma cell line) Whole Cell Lysate at 10 µg

  Lane 2: Western blot - HeLa whole cell lysate (ab150035) at 10 µg

  Developed using the ECL technique.

  Performed under reducing conditions.

  Predicted band size: 47 kDa

  Observed band size: 62 kDa

  Exposure time: 20s

• 

  Western blot - HeLa whole cell lysate (ab150035)

  This blot was produced using a 4-12% Bis-tris gel under the MES buffer system. The gel was run at 200V for 35 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 3% milk before being incubated with HRP Anti-Peroxiredoxin 2/PRP antibody [EPR5154] ab197042 overnight at 4°C. Antibody binding was visualised using ECL development solution ECL Substrate Kit (High Sensitivity) ab133406.

  All lanes: Western blot - HRP Anti-Peroxiredoxin 2/PRP antibody [EPR5154] (HRP Anti-Peroxiredoxin 2/PRP antibody [EPR5154] ab197042) at 1/3000 dilution

  Lane 1: HEK-293 (Human) Whole Cell Lysate (ab52256) at 10 µg

  Lane 2: Western blot - HeLa whole cell lysate (ab150035) at 10 µg

  Lane 3: LNCaP (Human prostate adenocarcinoma cell line) Whole Cell Lysate - clone FGC at 10 µg

  Lane 4: SHSY-5Y (Human neuroblastoma cell line) Whole Cell Lysate at 10 µg

  Developed using the ECL technique.

  Performed under reducing conditions.

  Predicted band size: 22 kDa

  Observed band size: 22 kDa

  Exposure time: 4min

• 

  Western blot - HeLa whole cell lysate (ab150035)

  This blot was produced using a 3-8% Tris Acetate gel under the TA buffer system. The gel was run at 150V for 60 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 2% Bovine Serum Albumin before being incubated with HRP Anti-BRG1 antibody [EPNCIR111A] ab196315 overnight at 4°C. Antibody binding was visualised using ECL development solution ECL Substrate Kit (High Sensitivity) ab133406.

  All lanes: Western blot - HRP Anti-BRG1 antibody [EPNCIR111A] (HRP Anti-BRG1 antibody [EPNCIR111A] ab196315) at 1/5000 dilution

  Lane 1: K562 (Human erythromyeloblastoid leukemia cell line) Whole Cell Lysate at 10 µg

  Lane 2: Western blot - HeLa whole cell lysate (ab150035) at 10 µg

  Lane 3: NIH 3T3 (Mouse embryonic fibroblast cell line) Whole Cell Lysate at 10 µg

  Lane 4: PC12 (Rat adrenal pheochromocytoma cell line) Whole Cell Lysate at 10 µg

  Developed using the ECL technique.

  Performed under reducing conditions.

  Predicted band size: 185 kDa

  Observed band size: 185 kDa

  Exposure time: 20min

• 

  Western blot - HeLa whole cell lysate (ab150035)

  This blot was produced using a 4-12% Bis-tris gel under the MES buffer system. The gel was run at 200V for 35 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 3% milk before being incubated with HRP Anti-RAB8A antibody [EPR14873] - C-terminal ab209046 overnight at 4°C. Antibody binding was visualised using ECL development solution ECL Substrate Kit (High Sensitivity) ab133406.

  All lanes: Western blot - HRP Anti-RAB8A antibody [EPR14873] - C-terminal (HRP Anti-RAB8A antibody [EPR14873] - C-terminal ab209046) at 1/5000 dilution

  Lane 1: Western blot - HeLa whole cell lysate (ab150035) at 10 µg

  Lane 2: HCT 116 (Human Colorectal Carcinoma) Whole Cell Lysate at 10 µg

  Lane 3: PC12 (Rat adrenal pheochromocytoma cell line) Whole Cell Lysate at 10 µg

  Lane 4: Wild-type HAP1 cell lysate at 20 µg

  Lane 5: RAB8A knockout HAP1 cell lysate at 20 µg

  Developed using the ECL technique.

  Performed under reducing conditions.

  Predicted band size: 24 kDa

  Observed band size: 24 kDa

  Exposure time: 20min

• 

  Western blot - HeLa whole cell lysate (ab150035)

  This blot was produced using a 4-12% Bis-tris gel under the MOPS buffer system. The gel was run at 200V for 50 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 3% milk before being incubated with HRP Anti-Cytochrome P450 17A1/CYP17A1 antibody [EPR6293] ab204022 overnight at 4°C. Antibody binding was visualised using ECL development solution ECL Substrate Kit (High Sensitivity) ab133406.

  All lanes: Western blot - HRP Anti-Cytochrome P450 17A1/CYP17A1 antibody [EPR6293] (HRP Anti-Cytochrome P450 17A1/CYP17A1 antibody [EPR6293] ab204022) at 1/5000 dilution

  Lane 1: Western blot - HeLa whole cell lysate (ab150035) at 10 µg

  Lane 2: Jurkat (Human T cell lymphoblast-like cell line) Whole Cell Lysate at 10 µg

  Developed using the ECL technique.

  Performed under reducing conditions.

  Predicted band size: 57 kDa

  Observed band size: 57 kDa

  Exposure time: 3min

• 

  Western blot - HeLa whole cell lysate (ab150035)

  All lanes: Western blot - Anti-p53 (phospho S15) antibody (Anti-p53 (phospho S15) antibody ab1431)

  All lanes: Western blot - HeLa whole cell lysate (ab150035)